Comparison of HPV sign Genotyping Test with INNO-LiPA HPV Genotyping Extra assay on histologic and cytologic cervical specimens

Barbieri, Daniela; Nocera, Martina; Gallinella, Giorgio; Gentilomi, Giovanna Angela; Plazzi, Marinella; Costa, S.; Santini, Donatella; Venturoli, Simona

doi:10.1016/j.diagmicrobio.2012.05.014

Cited by 17 publications

(13 citation statements)

References 26 publications

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“…This feature represents an advantage over methods based on the analysis of very short sequences, such as the commercially available HPV sign ® Genotyping test (Qiagen), which is based on broad‐spectrum amplification of a variable region of HPV L1 and pyrosequencing of short reads from the amplicons on the Pyromark Q24 system (Qiagen). In addition, the HPV sign ® Genotyping test was demonstrated to have a low sensitivity for some HPV types and for the discrimination of HPV types in multiple infections, but, on the other hand, being based on sequencing of short amplicons, the assay was shown to be suitable for testing archival formalin‐fixed paraffin‐embedded biopsies .…”

Section: Discussionmentioning

confidence: 99%

Accurate human papillomavirus genotyping by 454 pyrosequencing

Militello¹,

Lavezzo²,

Costanzi³

et al. 2013

Clinical Microbiology and Infection

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Accurate HPV typing is essential for evaluation and monitoring of HPV vaccines, for second-line testing in cervical cancer screening, and in epidemiological surveys. In this study, we set up and assessed in clinical samples a new HPV typing method based on 454 next-generation sequencing (NGS) of HPV L1 amplicons, generated by using a modified PGMY primer set with improved sensitivity for some HPV types that are not targeted by standard PGMY primers. By using a median 12 800-fold coverage, the NGS method allowed us to correctly identify all high-risk HPV types, in either single or multiple infections, with a sensitivity of 50 genome equivalents, as demonstrated by testing WHO LabNet EQA sample panels. Analysis of mixtures of HPV16- and HPV18-positive cell lines demonstrated that the NGS method could reproducibly quantify the proportion of each HPV type in multiple infections in a wide dynamic range. Testing of HPV-positive clinical samples showed that NGS could correctly identify a high number of HPV types in multiple infections. The NGS method was also effective in the analysis of a set of cervical specimens with discordant results at hybrid capture 2 and line probe assays. In conclusion, a new HPV typing method based on 454 pyrosequencing was set up. This method was sensitive, specific, quantitative and precise in both single and multiple infections. It could identify a wide range of HPV types and might potentially discover new HPV types.

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Section: Discussionmentioning

confidence: 99%

Accurate human papillomavirus genotyping by 454 pyrosequencing

Militello¹,

Lavezzo²,

Costanzi³

et al. 2013

Clinical Microbiology and Infection

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“…HPV genotyping was performed using a Rotor‐Gene Q Real‐Time PCR Cycler (Qiagen) according to a previously described method . Samples with HPV genotypes detected were further analyzed with pyrosequencing using the PyroMark Q24 System (Qiagen) and four specific sequencing primers for viral genotypes.…”

Section: Methodsmentioning

confidence: 99%

Age‐related changes in pre‐ and post‐conization HPV genotype distribution among women with high‐grade cervical intraepithelial neoplasia

Giannella

Fodero

Boselli

et al. 2017

Intl J Gynecology & Obste

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“…The INNO-LiPA HPV genotyping assay is capable of detecting and genotyping 25 different HPV types at the same time and has proven to be sensitive, specific, simple, and rapid in the determination of HPV (Chinchai et al, 2011;Barbieri et al, 2012). Detection of HPV is dependent on the methods employed it cannot be cultured in vitro and serological assays lack sensitivity and specificity.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Type Distribution of Human Papillomavirus Infection Using the INNo-Lipa Assay, Kerman, Southeast Iran

Afshar

Mollaie²,

Fazlalipour³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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The human papilloma virus (HPV) causes skin and mucous membrane infections. It crosses from one person to another by skin-to-skin contact, such as sexual contact. There are more than 100 types of HPV that can influence different parts of the body. Some types of HPV can cause cancer (such as cervical or anal cancer) and others can cause warts (such as genital or plantar warts). HPV infection is one of the most common sexually transmitted infections (STIs) in Iran and around the world. Considerable molecular evidence suggests a role for human papilloma virus (HPV) in the pathogenesis of carcinoma. Epidemiological studies on human papilloma viruses (HPVs) infections in general population are critical for the performing of health policy guidelines for developing the strategies to hinder the primary and secondary different cancer. In different parts of Iran, there is a lack of population-based studies to determine the prevalence of HPV in the general population. The aim of this population-based study was therefore to report the prevalence ratse of HPV types among Iranian patients. To study the risk of human papilloma virus (HPV) infection, we managed a retrospective study in Kerman province, southeast of Iran. For this purpose, 410 patients tested for the presence of HPV DNA using PCR and INNo-Lipa assays. HPV DNA was detected in 108 out of 410 patients (26.34%), while it was not detected in any of the control group samples. Patients included 23 (21.1%) males and 86 (78.8%) females. HPV type 6 was the most common (49%) followed by HPV type 16 (10.1%), and also HPV type11 (9.2%). The prevalence of HPV in Iran is comparable to those reported in other regions of the world. In a similar manner, it seems that HPV types 6, 16 and11 are the most common types in Kerman. Additional studies on larger group of patients, particularly in those with pre-invasive forms of disease, are needed to explain the roles of different HPV types in this location of Iran.

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Comparison of HPV sign Genotyping Test with INNO-LiPA HPV Genotyping Extra assay on histologic and cytologic cervical specimens

Cited by 17 publications

References 26 publications

Accurate human papillomavirus genotyping by 454 pyrosequencing

Accurate human papillomavirus genotyping by 454 pyrosequencing

Age‐related changes in pre‐ and post‐conization HPV genotype distribution among women with high‐grade cervical intraepithelial neoplasia

Prevalence and Type Distribution of Human Papillomavirus Infection Using the INNo-Lipa Assay, Kerman, Southeast Iran

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