Comparison of Histocompatibility Antigens on Cultured Human Tumor Cells and Fibroblasts by Quantitative Antibody Absorption and Sensitivity to Cell-Mediated Cytotoxicity
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Golub, Sidney H.; Hanson, Douglas C.; Sulit, Hector L.; Morton, Donald L.; Pellegrino, Michele; Ferrone, Soldano

doi:10.1093/jnci/56.1.167

Cited by 23 publications

(13 citation statements)

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“…The human cutaneous melanoma cell line 1007 was derived from primary melanoma (33). The melanoma cell lines SK-Mel 28 (American Type Culture Collection, Rockville, MD), M10, and Mel120 were derived from metastatic lesions (34). Cells were grown in RPMI 1640 containing 10% FCS.…”

Section: Methodsmentioning

confidence: 99%

Endothelin-1 and Endothelin-3 Promote Invasive Behavior via Hypoxia-Inducible Factor-1α in Human Melanoma Cells

Spinella¹,

Rosanò²,

Castro³

et al. 2007

Cancer Research

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Endothelin (ET) B receptor (ET B R), which is overexpressed in human cutaneous melanomas, promotes tumorigenesis upon activation by ET-1 or ET-3, thus representing a potential novel therapeutic target. Hypoxia-inducible factor-1A (HIF-1A) is the transcriptional factor that conveys signaling elicited by hypoxia and growth factor receptors. Here, we investigated the interplay between ET axis and hypoxia in primary and metastatic melanoma cell lines. We report that under normoxic conditions, ET B R activation by ET-1/ET-3 enhances vascular endothelial growth factor (VEGF) up-regulation, cyclooxygenase (COX)-1/COX-2 protein expression and COX-2 promoter activity, prostaglandin E 2 (PGE 2 ) production, and do so to a greater extent under hypoxia. Moreover, COX-1/COX-2 inhibitors block ET-induced PGE 2 and VEGF secretion, matrix metalloproteinase (MMP) activation, and cell invasion, indicating that both enzymes function as downstream mediators of ET-induced invasive properties. The ET B R selective antagonist BQ788 or transfection with ET B R small interfering RNA (siRNA) block the ET-mediated effects. ETs also increase HIF-1A expression under both normoxic and hypoxic conditions and its silencing by siRNA desensitizes COX-2 transcriptional activity, PGE 2 and VEGF production, and MMP activation in response to ET-3, implicating, for the first time, HIF-1A/COX as downstream targets of ET B R signaling leading to invasiveness. In melanoma xenografts, specific ET B R antagonist suppresses tumor growth, neovascularization, and invasiveness-related factors. Collectively, these results identify a new mechanism whereby ET-1/ET-3/ET B R axis can promote and interact with the HIF-1A-dependent machinery to amplify the COX-mediated invasive behavior of melanoma. New therapeutic strategies using specific ET B R antagonist could provide an improved approach to the treatment of melanoma by inhibiting tumor growth and progression. [Cancer Res 2007;67(4):1725-34]

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Section: Methodsmentioning

confidence: 99%

Endothelin-1 and Endothelin-3 Promote Invasive Behavior via Hypoxia-Inducible Factor-1α in Human Melanoma Cells

Spinella¹,

Rosanò²,

Castro³

et al. 2007

Cancer Research

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“…Nine human melanoma cell lines were used in this study. M14 and M10 (Golub et al, 1976) were kindly provided by Dr. G. Zupi (Istituto Regina Elena, Rome, Italy); LCP-MEL, GL-MEL, and PD-Mel (Lacal et al, 2000) were kindly provided by Dr. Guadagni (Istituto Regina Elena); CR-Mel, PR-Mel, and CNMel (Alvino et al, 2002) were established in our laboratory; and SK-Mel-28 was obtained from the American Type Culture Collection (Manassas, VA). LCP-Mel was obtained from a primary melanoma; PR-Mel, M14, and M10 were established from cutaneous metastases; and the remaining cell lines were originated from noncutaneous metastases, including lymph node and organ metastases.…”

Section: Methodsmentioning

confidence: 99%

The Effect ofO⁶-Alkylguanine-DNA Alkyltransferase and Mismatch Repair Activities on the Sensitivity of Human Melanoma Cells to Temozolomide, 1,3-bis(2-Chloroethyl)1-nitrosourea, and Cisplatin

Pepponi¹,

Marra²,

Fuggetta³

et al. 2003

J Pharmacol Exp Ther

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The prognosis of advanced melanoma is generally poor, because this tumor commonly exhibits intrinsic or acquired resistance to chemotherapy. In an attempt to identify the underlying causes of this resistance, we studied the roles played by the DNA repair enzyme O 6 -alkylguanine-DNA alkyltransferase (OGAT) and the mismatch repair (MMR) system in the sensitivity of melanoma cells to temozolomide (TMZ), 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), or cis-diamminedichloroplatinum(II) (CDDP). To this end, OGAT levels and MMR efficiency of extracts of nine melanoma cell lines and selected clones derived from four of these lines were determined and correlated with the sensitivity of the respective cells to these drugs. The effectiveness of O 6 -benzylguanine (BG), a specific OGAT inhibitor, in potentiating TMZ-or BCNU-mediated cytotoxicity was also evaluated. Our results demonstrate that MMR efficiency and OGAT levels strongly affect melanoma cell sensitivity to TMZ. In MMR-proficient cells, a direct correlation between OGAT levels and TMZ IC 50 values was found. When OGAT activity was inhibited with BG, the sensitivity of these cells to TMZ increased and was then dictated largely by their MMR efficiency. MMR-deficient cells were highly resistant to the drug irrespective of their OGAT levels. Although OGAT activity and MMR status seemed to be the major determinants of melanoma sensitivity to TMZ, this was not the case for BCNU and CDDP; resistance to the latter drugs clearly involves processes other than the two DNA repair pathways analyzed in this study.

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“…M14 [29] was kindly provided by Dr G. Zupi (Istituto Regina Elena, Rome, Italy), PR-Mel [30] was established in our laboratory, whereas SK-Mel-28 was obtained from the American Type Culture Collection (Rockville, Maryland, USA). All the cell lines originated from melanoma metastases.…”

Section: Cell Linesmentioning

confidence: 99%

In vitro antitumour activity of resveratrol in human melanoma cells sensitive or resistant to temozolomide

et al. 2004

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Resveratrol, a polyphenol present in many plant species, exhibits a wide range of biological and pharmacological activities both in vitro and in vivo. It has been shown to exert a potent chemopreventive effect in carcinogenesis models and to induce cell growth inhibition and apoptosis in human tumour cells, including melanoma cells. Malignant melanoma is considered to be a chemotherapy-refractory tumour, and the commonly used anticancer drugs do not seem to modify the prognosis of metastatic disease. To further evaluate the therapeutic potential of resveratrol in the treatment of melanoma, we selected three human melanoma cell lines with different levels of resistance to temozolomide (TMZ), an antitumour triazene compound. The cell lines were subjected to resveratrol treatment and analysed for cell growth inhibition, cell cycle perturbation and apoptosis induction. We found that resveratrol markedly impaired proliferation of both the TMZ-sensitive M14 and the TMZ-resistant SK-Mel-28 and PR-Mel cell lines. The latter cell line was two-fold more resistant to the drug than M14 and SK-Mel-28 cells. The sensitivity of normal human keratinocytes to resveratrol was found to be significantly higher than that of M14 and SK-Mel-28 cells and similar to that of the PR-Mel cell line. This suggests a possible good in vivo therapeutic index for resveratrol. Our results also show that the growth-inhibitory effect of resveratrol on melanoma cells is mainly due to its ability to induce S-phase arrest and apoptosis. Taken together, our data indicate that resveratrol is an interesting candidate for the treatment of advanced melanoma.

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Comparison of Histocompatibility Antigens on Cultured Human Tumor Cells and Fibroblasts by Quantitative Antibody Absorption and Sensitivity to Cell-Mediated Cytotoxicity 2 3

Cited by 23 publications

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Endothelin-1 and Endothelin-3 Promote Invasive Behavior via Hypoxia-Inducible Factor-1α in Human Melanoma Cells

Endothelin-1 and Endothelin-3 Promote Invasive Behavior via Hypoxia-Inducible Factor-1α in Human Melanoma Cells

The Effect ofO⁶-Alkylguanine-DNA Alkyltransferase and Mismatch Repair Activities on the Sensitivity of Human Melanoma Cells to Temozolomide, 1,3-bis(2-Chloroethyl)1-nitrosourea, and Cisplatin

In vitro antitumour activity of resveratrol in human melanoma cells sensitive or resistant to temozolomide

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Comparison of Histocompatibility Antigens on Cultured Human Tumor Cells and Fibroblasts by Quantitative Antibody Absorption and Sensitivity to Cell-Mediated Cytotoxicity 2 3

Cited by 23 publications

References 0 publications

Endothelin-1 and Endothelin-3 Promote Invasive Behavior via Hypoxia-Inducible Factor-1α in Human Melanoma Cells

Endothelin-1 and Endothelin-3 Promote Invasive Behavior via Hypoxia-Inducible Factor-1α in Human Melanoma Cells

The Effect ofO6-Alkylguanine-DNA Alkyltransferase and Mismatch Repair Activities on the Sensitivity of Human Melanoma Cells to Temozolomide, 1,3-bis(2-Chloroethyl)1-nitrosourea, and Cisplatin

In vitro antitumour activity of resveratrol in human melanoma cells sensitive or resistant to temozolomide

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The Effect ofO⁶-Alkylguanine-DNA Alkyltransferase and Mismatch Repair Activities on the Sensitivity of Human Melanoma Cells to Temozolomide, 1,3-bis(2-Chloroethyl)1-nitrosourea, and Cisplatin