Comparison of hepatic drug metabolizing enzyme activities in several agricultural species

Ce, Short; Flory, W; Hsieh, Lily C.; Aranas, T.; Ou, S P; Weissinger, Judi

doi:10.1016/0742-8413(88)90053-9

Cited by 30 publications

(24 citation statements)

References 17 publications

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“…There are several studies reporting the in vitro metabolism of many P450 isoform model substrates in liver preparations from ruminants [46]. In our study, the CYP3A-dependent N-demethylations of erythromycin and ethylmorphine were significantly higher in small ruminants (GT > OV) than in CA, confirming previously published results [7,47,48,51,58]. However, no species-differences were observed in the hydroxylation of testosterone at the 6ß-and 2ß-positions, two reactions catalysed by CYP3A also in ruminants [30].…”

Section: Discussionsupporting

confidence: 82%

Effect of breed and gender on bovine liver cytochrome P450 3A (CYP3A) expression and inter-species comparison with other domestic ruminants

et al. 2005

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-The cytochrome P450 (P450) superfamily represents a group of relevant enzymes in the field of drug metabolism and several exogenous or constitutional factors contribute to regulate its expression. Cattle represent an important source of animal-derived food-products and studies concerning the P450 expression are needed for the extrapolation of pharmacotoxicological data from one species to another and for the evaluation of the consumer's risk associated with the consumption of harmful residues found in foodstuffs. In the present study, possible breed-, genderand species-differences in P4503A (the P450 subfamily more expressed in the human liver) expression were studied in vitro in Piedmontese (PDM) and Limousin (LIM) meat cattle breeds of both sexes and in domestic Ruminants (cattle, sheep and goats). Cytochrome P450 and P4503A contents as well as CYP3A-dependent drug metabolising enzymes (DME) were measured in liver microsomes. Significant lower levels of P450 (P < 0.001) and P4503A (P < 0.05) contents were observed in PDM vs. LIM of both sexes; the P4503A-dependent DME activities were significantly (P values ranging from 0.05 up to 0.001) higher in PDM cattle, particularly in males. A gendereffect in DME activities was noticed (P < 0.05) only in PDM male cattle. With regards to the species, the expression of both P4503A apoprotein and some of the related DME activities were more pronounced in sheep (P < 0.01 vs. cattle) and in goats (P < 0.05 vs. sheep; P < 0.01 vs. cattle) than in cattle. The significant differences in P4503A expression observed in LIM and PDM cattle are consistent with previously published data on strain-and breed-differences pointed out in rats and men. As far as a possible sex-effect is concerned, no clear-cut evidence is likely to be drawn. Finally, P4503A expression was more relevant in small ruminants. ruminants / liver drug metabolism / CYP3A / gender / breed

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Section: Discussionsupporting

confidence: 82%

Effect of breed and gender on bovine liver cytochrome P450 3A (CYP3A) expression and inter-species comparison with other domestic ruminants

et al. 2005

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“…The elimination half time of acetaminophen in this study appeared to be shorter than that reported for cattle (Grochowina and Janus, 2007;Ehsani-Kheradgerdi et al, 2011) and lambs (Sharifi et al, 2009). This result in consistent with a more active metabolism in goats than in cattle or sheep, with goats having a significantly higher activity of UDP-glucuronyltransferase (Short et al, 1988) and higher liver concentrations of cytochrome p-450, NADPH cytochrome c reductase, aldrin epoxidase, aminopyrine Ndemethylase, ethoxycoumarin O-deethylase, microsomal and cytosolic stilbene oxide (epoxide) hydrolase and glutathione s-transferase than sheep and cattle (Wisniewski et al, 1987). Acetaminophen is metabolized primarily by conjugation with sulfate and glucuronide and in goats the glucuronide metabolite is the predominant moiety in plasma (Ali et al, 1996).…”

Section: Discussionsupporting

confidence: 87%

Effect of tulathromycin on abomasal emptying rate in healthy lactating goats

Badawy

Amer

Constable

et al. 2014

Small Ruminant Research

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“…As far as the horse is concerned, the majority of the published investigations have focused on the fate of speci¢c classes of drugs (for a review see Schmid and Schmid, 1994) rather than on study of the in vitro metabolism of model substrates (Lakritz et al, 2000;Nebbia et al, 2004); in addition, the comparative approach has been adopted only very rarely (Chauret et al, 1997;Nebbia et al, 2003). Furthermore, although the rabbit has been the subject of numerous studies aimed at investigating interspecies di¡erences in XME activity, almost invariably the animals used belonged to the New Zealand White breed and have been included as laboratory species (Watkins and Klaassen, 1986;Short et al 1988).…”

Section: Introductionmentioning

confidence: 97%

“…It is well recognized that species-related di¡erences in XME expression may a¡ect not only the persistence in the body of drugs and poisons but also susceptibility to them (Nebbia, 2001). However, food-producing species have been the subject only of a limited number of studies (Watkins and Klaassen, 1986;Short et al, 1988;Nebbia et al, 2003;Sivapathasundaram et al, 2003), the majority of which have been concerned with cytochrome P450-dependent monooxygenases. In contrast, relatively less attention has been paid to hydrolytic and conjugative pathways.…”

Section: Introductionmentioning

confidence: 98%

Comparison of Hydrolytic and Conjugative Biotransformation Pathways in Horse, Cattle, Pig, Broiler Chick, Rabbit and Rat Liver Subcellullar Fractions

et al. 2006

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To complete a studyaimed at investigating the pattern of the basal activities of liver xenobioticmetabolizing enzymes in major and minor species intended for meat production, microsomal carboxylesterases and some conjugating enzyme activities were determined and compared in liver preparations from horses, cattle, pigs, rabbits and broiler chicks, using the rat as a reference species. Horses and broiler chicks exhibited a lower microsomal carboxylesterase activity towards indophenyl or p-nitrophenyl acetate than that measured in cattle or pig subfractions. Among food-producing species, the rate of glucuronidation of either 1-naphthol or p-nitrophenol was in the order pigs approximately rabbits > horses >> cattle > broiler chicks. The widest variations were observed in the acetylation capacity towards p-aminobenzoic acid or isoniazid, which in rabbits was 3-fold to 11-fold greater than that displayed by any other examined species; low but measurable activities were found in equine and bovine cytosols. The activity of cytosolic glutathione S-transferase (GST) accepting the general substrate 1-chloro-2,4-dinitrobenzene was significantly higher in rabbits, horses and pigs than in rat, broiler chicks and cattle. Finally, an uneven pattern of activity towards the other tested GST substrates - 3,4-dichloronitrobenzene, ethacrinic acid or 1,2-epoxybutane - was observed, possibly reflecting the species-related expression of different GST classes; in this respect, the conjugative capacity displayed by horses was higher than or comparable to that found in the other food-producing species.

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Comparison of hepatic drug metabolizing enzyme activities in several agricultural species

Cited by 30 publications

References 17 publications

Effect of breed and gender on bovine liver cytochrome P450 3A (CYP3A) expression and inter-species comparison with other domestic ruminants

Effect of breed and gender on bovine liver cytochrome P450 3A (CYP3A) expression and inter-species comparison with other domestic ruminants

Effect of tulathromycin on abomasal emptying rate in healthy lactating goats

Comparison of Hydrolytic and Conjugative Biotransformation Pathways in Horse, Cattle, Pig, Broiler Chick, Rabbit and Rat Liver Subcellullar Fractions

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