Comparison of Growth Factor Treatments on the Fibrochondrogenic Potential of Canine Fibroblast‐Like Synoviocytes for Meniscal Tissue Engineering

Spina, Jason; Warnock, Jennifer J.; Duesterdieck-Zellmer, Katja F.; Baltzer, Wendy I.; Ott, Jesse; Bay, Brian K.

doi:10.1111/j.1532-950x.2014.12170.x

Cited by 6 publications

(4 citation statements)

References 74 publications

(105 reference statements)

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“…Aggrecan within joint tissues is produced by chondrocytes [25] and fibroblast-like synoviocytes [26]. Although IGF1 is thought to be the major stimulator of aggrecan synthesis by articular cartilage cells [27] we did not demonstrate this effect of IGF1 used at the concentration observed in cartilage interstitial fluid [10].…”

Section: Discussionmentioning

confidence: 58%

Influence of IGF1, TGFβ1, bFGF and G-CSF/M-CSF on the mRNA levels of selected matrix proteins, cytokines, metalloproteinase 3 and TIMP1 in rat synovial membrane cells

Osiecka-Iwan

Moskalewski

Hyc

2016

Folia Histochem Cytobiol.

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The response of the studied genes represents a resultant activity of all major cell types building the synovial membrane, i.e. highly specialized synovial fibroblasts, macrophages, epithelial cells and adipocytes. The results of our study can improve understanding of synovial membrane responses to the intraarticular injections of platelet-rich plasma (PRP) used for the treatment of joint ailments, since PRP contains factors which are also present in CIF.

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Section: Discussionmentioning

confidence: 58%

Influence of IGF1, TGFβ1, bFGF and G-CSF/M-CSF on the mRNA levels of selected matrix proteins, cytokines, metalloproteinase 3 and TIMP1 in rat synovial membrane cells

Osiecka-Iwan

Moskalewski

Hyc

2016

Folia Histochem Cytobiol.

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“…Despite the poor chondrogenic capacity of canine MSCs, so far, only a few studies have investigated the effect of growth factors on canine MSC chondrogenesis [30, 41]. Russel et al observed no evidence of cartilage matrix deposition in canine adipose tissue-derived MSCs, even when BMP-2 was added to chondrogenic induction medium containing TGF-β3 [30].…”

Section: Discussionmentioning

confidence: 99%

Comparison of the effect of growth factors on chondrogenesis of canine mesenchymal stem cells

et al. 2019

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Mesenchymal stem cells (MSCs) are proposed to be useful in cartilage regenerative medicine, however, canine MSCs have been reported to show poor chondrogenic capacity. Therefore, optimal conditions for chondrogenic differentiation should be determined by mimicking the developmental process. We have previously established novel and superior canine MSCs named bone marrow peri-adipocyte cells (BM-PACs) and the objective of this study was to evaluate the effects of growth factors required for in vivo chondrogenesis using canine BM-PACs. Spheroids of BM-PACs were cultured in chondrogenic medium containing 10 n g/m l transforming growth factor-β1 (TGF-β1) with or without 100 n g/m l bone morphogenetic protein-2 (BMP-2), 100 n g/m l growth differentiation factor-5 (GDF-5) or 100 n g/m l insulin-like growth factor-1 (IGF-1). Chondrogenic differentiation was evaluated by the quantification of glycosaminoglycan and Safranin O staining for proteoglycan production. The expression of cartilage matrix or hypertrophic gene/protein was also evaluated by qPCR and immunohistochemistry. Spheroids in all groups were strongly stained with Safranin O. Although BMP-2 significantly increased glycosaminoglycan production, Safranin O-negative outer layer was formed and the mRNA expression of COL10 relating to cartilage hypertrophy was also significantly upregulated ( P <0.05). GDF-5 promoted the production of glycosaminoglycan and type II collagen without increasing COL10 mRNA expression. The supplementation of IGF-1 did not significantly affect cartilaginous and hypertrophic differentiation. Our results indicate that GDF-5 is a useful growth factor for the generation of articular cartilage from canine MSCs.

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“…Recently, several attempts have been made to regenerate fibrocartilaginous tissue with tissue engineering approaches [ 32 – 34 ]. Most of these studies emphasize on the optimization of scaffolds or growth factors used for fibrocartilage engineering.…”

Section: Discussionmentioning

confidence: 99%

Periodontal Ligament Mesenchymal Stromal Cells Increase Proliferation and Glycosaminoglycans Formation of Temporomandibular Joint Derived Fibrochondrocytes

Zhang

Guo

et al. 2014

BioMed Research International

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Objectives. Temporomandibular joint (TMJ) disorders are common disease in maxillofacial surgery. The aim of this study is to regenerate fibrocartilage with a mixture of TMJ fibrochondrocytes and periodontal ligament derived mesenchymal stem cells (PD-MSCs). Materials and Methods. Fibrochondrocytes and PD-MSC were cocultured (ratio 1 : 1) for 3 weeks. Histology and glycosaminoglycans (GAGs) assay were performed to examine the deposition of GAG. Green florescent protein (GFP) was used to track PD-MSC. Conditioned medium of PD-MSCs was collected to study the soluble factors. Gene expression of fibrochondrocytes cultured in conditioned medium was tested by quantitative PCR (qPCR). Results. Increased proliferation of TMJ-CH was observed in coculture pellets when compared to monoculture. Enhanced GAG production in cocultures was shown by histology and GAG quantification. Tracing of GFP revealed the fact that PD-MSC disappears after coculture with TMJ-CH for 3 weeks. In addition, conditioned medium of PD-MSC was also shown to increase the proliferation and GAG deposition of TMJ-CH. Meanwhile, results of qPCR demonstrated that conditioned medium enhanced the expression levels of matrix-related genes in TMJ-CH. Conclusions. Results from this study support the mechanism of MSC-chondrocyte interaction, in which MSCs act as secretor of soluble factors that stimulate proliferation and extracellular matrix deposition of chondrocytes.

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Comparison of Growth Factor Treatments on the Fibrochondrogenic Potential of Canine Fibroblast‐Like Synoviocytes for Meniscal Tissue Engineering

Cited by 6 publications

References 74 publications

Influence of IGF1, TGFβ1, bFGF and G-CSF/M-CSF on the mRNA levels of selected matrix proteins, cytokines, metalloproteinase 3 and TIMP1 in rat synovial membrane cells

Influence of IGF1, TGFβ1, bFGF and G-CSF/M-CSF on the mRNA levels of selected matrix proteins, cytokines, metalloproteinase 3 and TIMP1 in rat synovial membrane cells

Comparison of the effect of growth factors on chondrogenesis of canine mesenchymal stem cells

Periodontal Ligament Mesenchymal Stromal Cells Increase Proliferation and Glycosaminoglycans Formation of Temporomandibular Joint Derived Fibrochondrocytes

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