Comparison of growth and metabolism of avian osteoblasts on polished disks versus thin films of titanium alloy

Abstract

The purpose of this study was to evaluate the efficacy of using high vacuum, thermal evaporation to deposit thin films of Ti-6Al-4V onto plates for subsequent cell culture investigations. Osteoblastic response to thin-film coated plates was compared to that of cells grown on Ti alloy disk inserts and uncoated culture plates. The Ti alloy disks were polished, cleaned, and passivated following a commercial protocol for orthopedic implants. Mean surface roughness was 262 nm for the Ti alloy disks and 4.756 nm for the coated culture plates. Osteoblasts isolated from 16-day chick embryo calvariae were cultured on polystyrene, thin films, and disks. At confluence, the cells were cultured an additional 48 h and were evaluated for cell number (DNA content), rate of glycolysis (lactate production), alkaline phosphatase activity (ALPase), and collagenous (3H-proline hydroxylation) and noncollagenous protein synthesis. Cell morphology was similar for the controls, disks, and thin-film groups. DNA, lactate, cell layer ALPase, 3H-hydroxyproline, and noncollagenous protein were not different (p > 0.05) among the control, thin-film, and disk groups. Medium ALPase was lower (p < 0.05) in the thin-film group compared to the control group. Although aluminum and vanadium percentages varied from nominal in the thin-film groups (11Al-2V as opposed to 6Al-4V), avian osteoblasts responded similarly to the Ti alloy thin films, disks, and uncoated culture plates for the smooth surfaces tested. The thin-film cell culture system used for elemental material studies appears to offer a promising method for the investigation of cellular response to alloyed biomaterials as well. Proper adjustments in alloy percentages before deposition, however, need to be made if thermal evaporation is utilized.

Alloy Design and Property Evaluation of New .BETA. Type Titanium Alloy with Excellent Cold Workability and Biocompatibility

“…titanium or Ti-6Al-4V, and much higher level over the type 316L. The effect of surface finish on the cell viability 29) was not observed in this study except pure nickel. This seems to indicate that the passive film might be recovered immediately after immersion into the culture media following mechanical polish.…”

Staphylococcus aureus adhesion to bone matrix and bone-associated biomaterials

“…The combination of molecular biology and experimental models of bacterial adhesion should resolve the mechanisms of S. aureus attachment to orthopedic implants. The recent development of titanium alloy culture surfaces [39] should facilitate studies of bacterial cell/host cell interactions with this and other biomaterials. Finally, speci¢c £uid-phase antiadhesive agents (e.g.…”

Polishing and coating carbon fiber‐reinforced carbon composites with a carbon‐titanium layer enhances adhesion and growth of osteoblast‐like MG63 cells and vascular smooth muscle cells in vitro

“…In the present study, we examined the adhesion and growth of cells in culture on CFRC composites polished with colloidal SiO 2 and coated with a carbon film enriched with titanium, a metal widely and successfully used in orthopedic and dental surgery. [20][21][22][23][24] The biocompatibility of artificial materials designed for or used in hard tissue surgery usually has been tested in terms of their interactions with osteoblasts and osteoclasts 6,[14][15][16][17][18][20][21][22][23][24][25][26][27][28] but more rarely with soft-tissue cells present in the bone or in close proximity to the bone, such as fibroblasts 17,19,29 or vascular cells, including endothelium, pericytes, and smooth muscle cells. [30][31][32][33] In titanium-coated bone implants, a vascular network has been seen in the grooves on the material surface, that is, in the interface between the implant and the newly formed bone.…”