Comparison of Gravitational SPLITT Fractionation with Gravitational Settling for Separating Micron Size Particles

Tantidanai, Nongnuch; Veerasai, Waret; Beckett, Ronald

doi:10.1080/01496390600786176

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…In order to obtain cut nanofiber samples with a more narrow length distribution, future processing efforts will incorporate a preparative-scale post-cutting separation step, such as split-flow thin-channel fractionation (SPLITT). [41] Noncovalent and Covalent Attachment of Alkaline Phosphatase to Aqueous Dispersed Nylon Nanofibers…”

Section: Resultsmentioning

confidence: 99%

Bioconjugation of Alkaline Phosphatase to Mechanically Processed, Aqueous Suspendible Electrospun Polymer Nanofibers for Use in Chemiluminescent Detection Assays

Mark

Stolper

Baratti

et al. 2008

Macromolecular Bioscience

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Aqueous suspendible polymer nanostructures were prepared by simple microtome processing of electrospun nylon 6 nanofibers and were used to immobilize calf intestinal alkaline phosphatase (ALP) by either covalent or noncovalent bioconjugation chemistries. It was found that noncovalent immobilization of ALP to the mechanically cut nanofibers (mean length approximately 4 microm; mean diameter approximately 80 nm) using a multi-stacked, layer-by-layer (LBL) approach with the cationic polymer Sapphire II resulted in the highest enzyme loading (48.1 +/- 0.4 microg . mg(-1) nanofiber) when compared to other covalent immobilization methods based on glutaraldehyde crosslinking. The biofunctionalized nanofibers were also characterized for their chemiluminescent activity with the dioxetane substrate, CSPD. The results indicate that the kinetic parameters, K(m) and V(max), for the catalytic activity of the nanostructure-bound ALP enzyme were influenced by the particular types of immobilization methods employed. In terms of the overall catalytic performance of the various immobilized ALP systems, a single-stacked LBL assembly approach resulted in the highest level of enzymatic activity per unit mass of nanofiber support. To the best of our knowledge, this study represents the first report examining the preparation of mechanically shortened, aqueous dispersed electrospun polymer nanofibers for potential application as enzyme scaffolds in chemiluminescent-based assay systems.

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Section: Resultsmentioning

confidence: 99%

Bioconjugation of Alkaline Phosphatase to Mechanically Processed, Aqueous Suspendible Electrospun Polymer Nanofibers for Use in Chemiluminescent Detection Assays

Mark

Stolper

Baratti

et al. 2008

Macromolecular Bioscience

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show abstract

“…Importantly, the method can potentially be adapted for the production of other types of hybrid polymer nanocomposite structures as well, including concentric-tubular composites and multi-segmented composite nanorods. In order to obtain nanorod samples with a more narrow length distribution, future processing efforts will incorporate a post-microfiltration preparative-scale separation step, such as split-flow thin-channel fractionation (SPLITT) [34].…”

Section: Preparation and Physical Characterization Of Alumina Membranmentioning

confidence: 99%

Biofunctionalization of aqueous dispersed, alumina membrane-templated polymer nanorods for use in enzymatic chemiluminescence assays

Mark¹,

Stolper²,

Baratti³

et al. 2008

Colloids and Surfaces B: Biointerfaces

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Extraction of unburned carbon from coal fly ash

Singh

Ghorai

Kar

2022

Handbook of Fly Ash

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Comparison of Gravitational SPLITT Fractionation with Gravitational Settling for Separating Micron Size Particles

Cited by 3 publications

References 12 publications

Bioconjugation of Alkaline Phosphatase to Mechanically Processed, Aqueous Suspendible Electrospun Polymer Nanofibers for Use in Chemiluminescent Detection Assays

Bioconjugation of Alkaline Phosphatase to Mechanically Processed, Aqueous Suspendible Electrospun Polymer Nanofibers for Use in Chemiluminescent Detection Assays

Biofunctionalization of aqueous dispersed, alumina membrane-templated polymer nanorods for use in enzymatic chemiluminescence assays

Extraction of unburned carbon from coal fly ash

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