Comparison of glycated hempglobin with HPLC and capillary electrophoresis

Warade, Jayesh

doi:10.18203/2320-6012.ijrms20171828

Cited by 4 publications

(2 citation statements)

References 9 publications

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“…In addition, CE is also preferred over HPLC in terms of easy experimental setup, faster time for analysis, and requires very low reagent and sample as well as cost effective. 20 …”

Section: Discussionmentioning

confidence: 99%

Significantly High HbA1c in Diabetic Patient with Hb J: Case Report

et al. 2022

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Glycated hemoglobin (HbA1c) is used to monitor the long-term management of diabetes and reflects the average blood glucose level over the past three months. Hb J is an alpha-globin gene variant that occurs less commonly but can interfere with the HbA1c result. This case report presents two cases of abnormally high HbA1c in patients with Hb J using the high-performance liquid chromatography (HPLC) method and repeated value using the capillary electrophoresis (CE) method. The first case was a 26 years old female Malay patient, presenting at 25 weeks gestation with diabetes mellitus (DM). Her HbA1c results from HPLC showed persistently high level (> 18.5%, > 179 mmol/mol) despite optimum diabetic control (fasting blood sugar (FBS) range 4.0–6.1 mmol/L). The second case was a 62-year-old female Malay with type 2 DM. Her HbA1c results from HPLC was also persistently high (> 18.5%, > 1;79 mmol/mol) despite good diabetic control (FBS average 5.0–7.0 mmol/L). Both patients’ hemoglobin analysis reports were suggestive of Hb J. Repeated HbA1c using CE were 6.0% (42 mmol/mol) and 8.1% (65 mmol/mol), respectively, and supported the presence of the Hb J variant peak. HbA1c measurement in patients with a variant should be interpreted with caution to avoid misdiagnosis and mismanagement in these kinds of patients.

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“…In addition, CE is also preferred over HPLC in terms of easy experimental setup, faster time for analysis, and requires very low reagent and sample as well as cost effective. 20 …”

Section: Discussionmentioning

confidence: 99%

Significantly High HbA1c in Diabetic Patient with Hb J: Case Report

et al. 2022

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“…Capillary Electrophoresis (CE) [29,59,124,136] • CE is a very precise and accurate method to estimate Hb fractions.…”

Section: Hplc Ion Exchangementioning

confidence: 99%

Standard of Care in Diagnosis of Diabetes Mellitus: HbA1c

2023

MCMS

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Objective: The HbA1c concentration is a crucial component of routine diabetes therapy since it serves both as a risk predictor and an indicator of long-term glycemia and represents the average glycemic history of the previous two to three months.The HbA1c assay was approved as the preferred approach for diagnosing diabetes by the American Diabetes Association, the European Association for the Study of Diabetes, and the International Diabetes Federation. This article provides an overview of HbA1c to comprehend its importance in the routine monitoring of diabetic patients. Topics discussed include measurement techniques, advantages and disadvantages of different measurement techniques, clinical significance of HbA1c, rapid diagnosis of HbA1c, and alternative means available to assess glycaemic control.Methods: This review highlights in detail the structure and formation of HbA1c, clinical importance factors influencing HbA1c levels, methods of measurement, the challenges associated with measuring the HbA1c concentration and describes the current state of the art of analytical and clinical aspects of the process.Results: Recent developments have made HbA1c assays more sensitive, simple to use, and less expensive. In comparison to previous technologies, the point-of-care HbA1c testing devices deliver quantifiable findings while limiting inference of other Hb variations, making diagnosis far more reliable. Conclusion:A person with diabetes can live a long and high -quality life if regular monitoring and treatment of diabetes should be done properly. This review emphasizes the shortcomings faced by recent diagnostics techniques and the correlation between falsely elevated and lowered HbA1c results with different physical conditions.

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Interference of hemoglobin variants in HbA1c quantification

Yadav

Mandal

2023

Clinica Chimica Acta

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Comparison of glycated hempglobin with HPLC and capillary electrophoresis

Cited by 4 publications

References 9 publications

Significantly High HbA1c in Diabetic Patient with Hb J: Case Report

Significantly High HbA1c in Diabetic Patient with Hb J: Case Report

Standard of Care in Diagnosis of Diabetes Mellitus: HbA1c

Interference of hemoglobin variants in HbA1c quantification

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