Comparison of glass slides and various digital‐slide modalities for cytopathology screening and interpretation

Hanna, Matthew G.; Monaco, Sara E.; Cuda, Jacqueline; Xing, Juan; Ahmed, Ishtiaque; Pantanowitz, Liron

doi:10.1002/cncy.21880

Cited by 62 publications

(71 citation statements)

References 12 publications

(18 reference statements)

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“…Higher intraobserver agreement was seen with cervical smears (Table ), approaching or even exceeding crude intraobserver agreement (85.6%) and intraobserver kappa scores (ranging from 0.47 to 0.81) reported in the literature for cervical‐vaginal smears . The greatest concordance and diagnostic accuracy would be expected using digital whole slide imaging for cytology specimens; one study showed 90%‐100% concordance between Aperio digital images and glass slides for thirty cases (10 ThinPrep gynecologic cases plus 20 nongynecologic cases) …”

Section: International Telecytology Studies: How Do They Compare To Amentioning

confidence: 82%

“…For 3‐dimensional groups, the plane of focus is very important, and automated scanners often produce images that are out of focus. An important solution to this problem has been the development of high magnification z‐axis image stacking, in which glass slides are scanned in multiple image planes along the z‐axis, resulting in a composite image of individual scans stacked on top of each other (a z‐stack) . Z‐stacking results in images that can be viewed at different planes, and allows for better focus of 3‐dimensional groups and improved visualization of individual cells.…”

Section: Overview Of Telecytologymentioning

confidence: 99%

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International telecytology: Current applications and future potential

Caron

Yan

et al. 2018

Diagnostic Cytopathology

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International telecytology can improve patient care by increasing access to regional and international expertise in cytopathology. The majority of international telecytology studies published to date have been based on static telepathology platforms. Overall concordance rates for these studies ranged from 71% to 93%. This is comparable to the concordance rates published for other studies comparing diagnoses made by digital still images to reference glass slides, which vary from 80% to 95%. Static telepathology systems are relatively cheap and easy to use, and have the potential to increase access to international experts in developing countries with limited resources. In contrast, resource-rich academic and private medical centers can use whole slide digital imaging (WSI) for telecytology consultation, though few studies have been published addressing this topic. International telepathology consultation services with digital whole slide image capabilities have been established at several academic medical centers including the University of Pittsburgh Medical Center (UPMC) and the University of California at Los Angeles (UCLA), through the UCLA Center for Telepathology and Digital Pathology. In a small series of 20 telecytology cases submitted to UCLA from 2014 to 2017 (10 gynecologic and 10 fine needle aspiration cases), a meaningful diagnosis was rendered for 100% of cases, with 100% concordance between the submitting institution, versus consultation diagnosis provided by UCLA. These limited results are promising, and in the future both WSI and static telecytology consultation may have a place serving clinical needs in different practice settings.

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Section: International Telecytology Studies: How Do They Compare To Amentioning

confidence: 82%

Section: Overview Of Telecytologymentioning

confidence: 99%

International telecytology: Current applications and future potential

Caron

Yan

et al. 2018

Diagnostic Cytopathology

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“…FDA approved telepathology systems, used for screening, primary diagnosis, second opinion, and education, can be designed as a static mode (images are captured, transmitted, and then viewed at distance), dynamic mode (live video images are transmitted and viewed at distance in real time), or dynamic robotic mode (when the viewer controls the microscope at the originating site) [17,18]. The practice of telepathology has been validated in surgical pathology (frozen section) and cytology [19,20 ▪▪ ,21–23], however, in renal pathology it is currently under testing. The two major indications that best fit the use of telepathology are assessment of adequacy of tissue procurement at the time of the renal biopsy (currently under validation at the University of Miami), and adequacy of renal parenchyma from frozen sections prior to transplantation.…”

Section: Virtual Microscopy In Clinical Practicementioning

confidence: 99%

Digital pathology in nephrology clinical trials, research, and pathology practice

Barisoni

Hodgin

2017

Current Opinion in Nephrology and Hypertension

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Purpose of review In this review, we will discuss (i) how the recent advancements in digital technology and computational engineering are currently applied to nephropathology in the setting of clinical research, trials, and practice; (ii) the benefits of the new digital environment; (iii) how recognizing its challenges provides opportunities for transformation; and (iv) nephropathology in the upcoming era of kidney precision and predictive medicine. Recent findings Recent studies highlighted how new standardized protocols facilitate the harmonization of digital pathology database infrastructure and morphologic, morphometric, and computer-aided quantitative analyses. Digital pathology enables robust protocols for clinical trials and research, with the potential to identify previously underused or unrecognized clinically useful parameters. The integration of digital pathology with molecular signatures is leading the way to establishing clinically relevant morphoomic taxonomies of renal diseases. Summary The introduction of digital pathology in clinical research and trials, and the progressive implementation of the modern software ecosystem, opens opportunities for the development of new predictive diagnostic paradigms and computer-aided algorithms, transforming the practice of renal disease into a modern computational science.

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“…Of the 100 cases scanned, 4 contained corrupted data caused by z-stacks that were not fully embedded on the main scan and therefore these cases were excluded from further analysis. The average data size per case of the 96 cases was 226 megabytes (range, 64.4-483.4 megabytes), the average number of focal 320 scans per case was 4.2 (range, [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15], and the mean number of embedded z-stacks per case was 6.15 (range, 0-14). The average image scanning time was 4.36 minutes per slide (range, 3:11-7:03 minutes per slide).…”

Section: Digital Cytology Case Analysismentioning

confidence: 99%