2014
DOI: 10.1186/1475-2875-13-174
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Comparison of Giemsa microscopy with nested PCR for the diagnosis of malaria in North Gondar, north-west Ethiopia

Abstract: BackgroundMalaria remains one of the leading communicable diseases in Ethiopia. Early diagnosis combined with prompt treatment is one of the main strategies for malaria prevention and control. Despite its limitation, Giemsa microscopy is still considered to be the gold standard for malaria diagnosis. This study aimed to compare the performance of Giemsa microscopy with nested polymerase chain reaction (nPCR) for the diagnosis of malaria in north-west Ethiopia.MethodsA cross sectional study was conducted in pub… Show more

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Cited by 45 publications
(55 citation statements)
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References 19 publications
(21 reference statements)
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“…This finding is not in consonance with the good measure of agreement with nested PCR observed by Alemu et al [20]. Microscopy is subjective and the result obtained is dependent on the quality of training of the observer.…”
Section: Discussioncontrasting
confidence: 60%
“…This finding is not in consonance with the good measure of agreement with nested PCR observed by Alemu et al [20]. Microscopy is subjective and the result obtained is dependent on the quality of training of the observer.…”
Section: Discussioncontrasting
confidence: 60%
“…Our results are in tandem agreement with previous studies showing that molecular detection techniques have greater sensitivity and specificity than either microscopy for the identification of single and mixed species infections [23,[39][40][41][42], as well as sub-microscopic infections [11,43]. The detection of Plasmodium parasites at very low density parasitaemia is difficult by microscopy and thus requires a molecular approach especially for surveillance or epidemiological studies [44]. Current methods which rely on PCR-based assays of ribosomal RNA genes to detect circulating parasites have been suggested to represent the overall gold standard of malaria diagnostics [45], and is increasingly used in epidemiological studies.…”
Section: Discussionsupporting
confidence: 82%
“…Polymerase chain reaction (PCR) in its various formats has emerged as the most sensitive method able to detect low levels of parasites in the blood especially in this setting [36, 37]. However, PCR requires high capital investment costs, service agreements, reagent supplies, and trained staff in molecular technologies with robust quality assurance programmes.…”
Section: Discussionmentioning
confidence: 99%