Comparison of gene expression of immune mediators in lung and pulmonary lymph node granulomas from cattle experimentally infected with Mycobacterium bovis

Shu, Dairu; Heiser, Axel; Wedlock, D. Neil; Luo, Dongwen; Lisle, Geoffrey W. de; Buddle, Bryce M.

doi:10.1016/j.vetimm.2014.03.017

Cited by 21 publications

(22 citation statements)

References 41 publications

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“…A subset of T cells, known as Th17 cells, produces IL-17A. Using qPCR, IL-17A has been detected in bovine tuberculoid lymph node and pulmonary granulomas (Aranday-Cortes et al, 2012a;Shu et al, 2014). IL-17A has been also shown to play a role in the immunopathology of other chronic diseases (Reynolds et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…the tuberculin skin test) and ex vivo (interferon [IFN]-g release assays) as well as ex-vivo proliferative responses of peripheral blood mononuclear cells (PBMCs) stimulated with M. bovis-specific or other relevant antigens (Pollock et al, 1996(Pollock et al, , 2000(Pollock et al, , 2001(Pollock et al, , 2003Waters et al, , 2006aWaters et al, , 2006bWaters et al, , 2006c. More targeted investigations have examined tissues or organs containing tuberculoid granulomas, focusing on regions within or near lesions (Widdison et al, 2006(Widdison et al, , 2009Shu et al, 2014). Others have focused on the granuloma itself, through the use of immunohistochemistry (IHC) or laser capture microdissection (LCM) combined with real time quantitative polymerase chain reaction (qPCR) (Palmer et al, 1999;Wangoo et al, 2005;Johnson et al, 2006Johnson et al, , 2007Aranday-Cortes et al, 2012a).…”

Section: Introductionmentioning

confidence: 99%

“…Few studies have examined cytokine expression at the primary site of infection (i.e. the lung) using a model that closely mimics natural infection (Shu et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Other cytokines and chemokines shown to be associated with an anti-mycobacterial response in cattle include tumour necrosis factor (TNF)-a, interleukin (IL)-17A, CXCL9 and CXCL10 (Thacker et al, 2007;Aranday-Cortes et al, 2012a;Shu et al, 2014). Using lymph nodes from experimentally infected cattle, one study showed that the expression of certain cytokines and chemokines varied among different stages of granuloma development (Aranday-Cortes et al, 2012a).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Cytokine Gene Expression using a Novel Chromogenic In-situ Hybridization Method in Pulmonary Granulomas of Cattle Infected Experimentally by Aerosolized Mycobacterium bovis

Palmer

Thacker

Waters

2015

Journal of Comparative Pathology

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Mycobacterium bovis is the cause of tuberculosis in most animal species including cattle and is a serious zoonotic pathogen. In man, M. bovis infection can result in disease clinically indistinguishable from that caused by Mycobacterium tuberculosis, the cause of most human tuberculosis. Regardless of host, the typical lesion induced by M. bovis or M. tuberculosis is the tuberculoid granuloma. Tuberculoid granulomas are dynamic structures reflecting the interface between host and pathogen and, therefore, pass through various morphological stages (I to IV). Using a novel in-situ hybridization assay, transcription of various cytokine and chemokine genes was examined qualitatively and quantitatively using image analysis. In experimentally infected cattle, pulmonary granulomas of all stages were examined 150 days after aerosol exposure to M. bovis. Expression of mRNA encoding tumour necrosis factor (TNF)-α, transforming growth factor-β, interferon (IFN)-γ, interleukin (IL)-17A, IL-16, IL-10, CXCL9 and CXCL10 did not differ significantly between granulomas of different stages. However, relative expression of the various cytokines was characteristic of a Th1 response, with high TNF-α and IFN-γ expression and low IL-10 expression. Expression of IL-16 and the chemokines CXCL9 and CXCL10 was high, suggestive of granulomas actively involved in T-cell chemotaxis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Few studies have examined cytokine expression at the primary site of infection (i.e. the lung) using a model that closely mimics natural infection (Shu et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Analysis of Cytokine Gene Expression using a Novel Chromogenic In-situ Hybridization Method in Pulmonary Granulomas of Cattle Infected Experimentally by Aerosolized Mycobacterium bovis

Palmer

Thacker

Waters

2015

Journal of Comparative Pathology

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“…This stage is characterised by multiple coalescing caseonecrotic granulomas. A recent comparison of gene expression of cytokines and immune mediators in the primary granuloma in the lung and in the draining pulmonary lymph nodes of M. bovis-infected cattle indicated a stronger pro-inflammatory immune response in the lung granulomas [11]. This may be a consequence of enhanced expression of interferon regulatory factor 5 (IRF5) promoting IFN-γ and IL-17 production.…”

Section: Immunity Against M Bovis Infection In Cattlementioning

confidence: 99%

Immunity and Vaccination against Tuberculosis in Cattle

Parlane¹,

Buddle²

2015

Curr Clin Micro Rpt

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Bovine tuberculosis (TB) continues to be a major animal health problem worldwide and new tools are required for the control of this disease in cattle, particularly with the emergence of wildlife reservoirs for Mycobacterium bovis infection and potential restrictions on trade. New insights into the roles of macrophages, dendritic cells, NK cells, and CD4+, CD8+, and γδ T cells in control of M. bovis infection in cattle have provided a better understanding of protective immunity against this disease. In the past two decades, considerable progress has been made in the development and evaluation of TB vaccines for cattle, with new attenuated mycobacterial vaccines providing an alternative to the use of BCG vaccine and sub-unit vaccines to boost protection induced by BCG. With the development of tests to differentiate infected from vaccinated animals, it is now feasible to use vaccines to assist in the control of this disease.

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