Comparison of gene expression changes induced by exposure to Ag, Cu-TiO2, and TiO2 nanoparticles in zebrafish embryos

Park, Hyung-Geun; Yeo, Min‐Kyeong

doi:10.1007/s13273-013-0017-0

Cited by 26 publications

(21 citation statements)

References 37 publications

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“…No larval zebrafish died after exposure to 2 mg/L of TiO 2 NPs or bulk TiO 2 , which is consistent with previous studies of TiO 2 NP toxicology (Boyle et al ; Boran et al ). Exposure of zebrafish to TiO 2 for 24 h did not cause any induction of mt2 , which is in accordance with previous investigation of mt2 gene expression in zebrafish after exposure to TiO 2 NPs (Park and Yeo ; Tan and Wang ; Boran et al ). It has been observed that organometal halide cells in the aqueous phase can release the metal halide (e.g., PbI 2 ) and cause mortality in zebrafish larvae at high concentrations (Babayigit et al ; Benmessaoud et al ); however, the low perovskite concentrations tested in the present study did not cause toxicity to C. vulgaris or zebrafish larvae.…”

Section: Discussionsupporting

confidence: 92%

Differences in Engineered Nanoparticle Surface Physicochemistry Revealed by Investigation of Changes in Copper Bioavailability During Sorption to Nanoparticles in the Aqueous Phase

Patsiou

Kálmán

Fernandes

et al. 2019

Enviro Toxic and Chemistry

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Sorption of chemical substances to nanoparticles (NPs) in the aqueous phase strongly influences NP physicochemisty, and investigations of these complex interactions can provide important insights into the environmental fate of NPs. The objective of the present study was to use differences in copper (Cu) bioavailability to investigate aqueous‐phase sorption with NPs that had different physicochemical characteristics (silicon [Si], perovskite, and titanium dioxide NPs [TiO2 NPs]). Sorption of Cu with NPs was assessed by the presence of adsorbent in water and onto the NP surface after ultracentrifugation, and by changes in Cu bioavailability under static conditions during exposure of larval zebrafish, as well as under conditions of continuous agitation during exposure of the alga Chlorella vulgaris. The presence of TiO2 NPs reduced total Cu in the water column and Cu bioavailability (measured by growth inhibition, mortality, and metallothionein 2 gene expression), confirming Cu sorption to TiO2 NPs. Nanoparticle surface area was the most important factor that affected Cu sorption, as indicated by less bioavailable Cu in the presence of smaller TiO2 NPs. The surface area effect was consistent regardless of exposure conditions (alga, continuous agitation; zebrafish, static water) and was further supported by the fact that the lowest total Cu concentration in the water column was found in the presence of the smallest NP. The results differed with other NP types, for example, silicon NPs, in which Cu sorption was indicated by analytical chemistry, but sorption was not sufficient to significantly alter Cu bioavailability. The bioavailability tests did not indicate Cu sorption with perovskite NPs. The results demonstrate that surface area critically influences sorption, that Cu sorption as measured by bioavailability is not affected by agitation or static conditions, and that Cu sorption differs among types of NPs, indicating differences in their surface physicochemistry. Environ Toxicol Chem 2019;9999:1–11. © 2019 SETAC

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Section: Discussionsupporting

confidence: 92%

Differences in Engineered Nanoparticle Surface Physicochemistry Revealed by Investigation of Changes in Copper Bioavailability During Sorption to Nanoparticles in the Aqueous Phase

Patsiou

Kálmán

Fernandes

et al. 2019

Enviro Toxic and Chemistry

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“…Differential gene expression in zebrafish embryos has been shown to be a sensitive endpoint in studies with metal-based nanoparticles Griffitt et al, 2013;Park and Yeo, 2013;Yeo and Park, 2012). However, no effect of Carbo-Iron on gene expression levels was detected in the present study.…”

Section: Tablecontrasting

confidence: 68%

“…Potential uptake of Carbo-Iron into the test organisms was evaluated by microscopic analysis. Since gene expression analysis is a sensitive method for detecting sublethal stress responses in fish and was used successfully to observe effects of different nanoparticles (Chae et al, 2009;Chen et al, 2011;Choi et al, 2010;Park and Yeo, 2013), differential gene expression in zebrafish embryos was analyzed with microarrays.…”

Section: Introductionmentioning

confidence: 99%

The oxidized state of the nanocomposite Carbo-Iron® causes no adverse effects on growth, survival and differential gene expression in zebrafish

Weil

Meißner

Busch

et al. 2015

Science of The Total Environment

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“…In fact, the transcription of the caspase gene is more important in the digestive gland of mussels. Park and Yeo (2013) identified 314 genes (278 upregulated and 36 downregulated) in zebra fish Danio rerio embryos exposed to Ag NPs (20-30 nm in diameter, 20 ppt). The number of genes involved in apoptosis (151 of the upregulated genes) far exceeded the contribution (54 %) of other genes involved in immune response (18 %), heat-shock protein activity (12 %), and endocytosis (10 %).…”

Section: Caspase Gene Transcription Profilementioning

confidence: 99%

Is gene transcription in mussel gills altered after exposure to Ag nanoparticles?

Bebianno

González-Rey

Gomes

et al. 2015

Environ Sci Pollut Res

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Nanotechnology is a rapid field of development with the enhancement of the production of different types of nanoparticles (NPs) applied in several industrial and commercial applications which increase the risk of their presence in the aquatic environment. Ag NPs have a wide application in everyday life products. However, there is concern about the exposure effects on aquatic organisms to these NPs. Therefore, this study aims to assess gene transcription alterations in mussels Mytilus galloprovincialis gills exposed for 2 weeks to Ag NPs (42 ± 10 nm, 10 μg.L(-1)). The genes were selected based on previous biomarkers and proteomic results and included superoxide dismutase (SOD), catalase (CAT), glutathione transferase (GST), caspase 3/7-1 (CAS), cathepsin L (CATH), heat-shock protein 70 (HSP 70), cytochrome P450 4YA (CYP 4YA), the elongation factor (EF1), actin and α- tubulin. No significant changes in gene transcription profiles were observed after exposure of M. galloprovincialis to Ag NPs for 15 days. The lack of significant gene transcription responses is in light with previous results obtained for mussels exposed to these NPs and may be related to the fact that enzyme kinetics and relative abundance of proteins (increase of antioxidant enzymes and metalllothioneins (MTs) with the time of exposure) do not always directly reflect their relative mRNA levels. Nevertheless, their overall expression maintenance may signify that, at end of the exposure period (15 days), the transcription of the respective genes is no longer required, pointing out to a possible adaptation effect to nanoparticles or due to the levels of Ag NPs accumulated in this tissue at this exposure time. This study highlights that gene transcription application and role as an additional and/or alternative end point approach is important to understand the mode of action of these emergent contaminants in aquatic organisms. However, in future studies, the time window needs to be adjusted, as genes are likely to respond earlier to the nanoparticle exposure.

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Comparison of gene expression changes induced by exposure to Ag, Cu-TiO2, and TiO2 nanoparticles in zebrafish embryos

Cited by 26 publications

References 37 publications

Differences in Engineered Nanoparticle Surface Physicochemistry Revealed by Investigation of Changes in Copper Bioavailability During Sorption to Nanoparticles in the Aqueous Phase

Differences in Engineered Nanoparticle Surface Physicochemistry Revealed by Investigation of Changes in Copper Bioavailability During Sorption to Nanoparticles in the Aqueous Phase

The oxidized state of the nanocomposite Carbo-Iron® causes no adverse effects on growth, survival and differential gene expression in zebrafish

Is gene transcription in mussel gills altered after exposure to Ag nanoparticles?

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