Comparison of fused-core and conventional particle size columns by LC–MS/MS and UV: Application to pharmacokinetic study

Song, Wei; Pabbisetty, Deepthi; Groeber, Elizabeth; Steenwyk, Rick C.; Fast, Douglas M.

doi:10.1016/j.jpba.2009.05.011

Cited by 48 publications

(20 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2a). Although the new method demonstrated benefits expected with SPP columns [9,12,13] (e.g., increased efficiency, reduced runtime, and increased signal/noise), a minor selectivity change was observed (Fig. 2b, c).…”

Section: Resultsmentioning

confidence: 84%

Pre-Validation and Performance Prediction Using Pressure Monitoring to Evaluate HPLC Method Development Changes

Wood

Peoples

2012

Chromatographia

View full text Add to dashboard Cite

HPLC methods for pharmaceutical analysis evolve from method development to commercial product testing. The majority of development and validation takes place in an R&D setting, followed by transfer to commercial sites that execute test methods on a routine basis. There is a growing need to increase confidence and probability that developed methods will be successful both during validation and long-term use with products, particularly when introducing new or unfamiliar technologies. An HPLC method pre-validation and performance prediction plan is presented using model pharmaceutical ingredients in prototype liquid formulations. The pre-validation includes limited linearity, repeatability in sample matrix, precision, carry-over, and selectivity. The performance prediction involves injecting large numbers of worst-case samples and monitoring five critical parameters: retention time, tailing factor, resolution, efficiency, and system pressure. Parameter results are assessed with respect to precision and/or system performance. In a proof of principle model, the performance prediction data demonstrate that system suitability parameters remain constant during injections of prepared samples; however, the system pressure increases over time. These underlying data indicate a potential trend prior to method validation. As a result, sample preparation and HPLC condition modifications are evaluated using pressure plots as a key performance metric.

show abstract

Section: Resultsmentioning

confidence: 84%

Pre-Validation and Performance Prediction Using Pressure Monitoring to Evaluate HPLC Method Development Changes

Wood

Peoples

2012

Chromatographia

View full text Add to dashboard Cite

show abstract

“…Ascentis ® Express column, which is packed with unique 2.7-µm fused-core particles, 23 showed the best performance, i.e., better resolution and peak sharpness with a low back pressure compared to other conventional particulate columns. [24][25][26] Accordingly, almost all constituents of P. koreana extract, including the four triterpenoid saponins, were fairly well resolved within 26 min of elution ( Figure 5). …”

Section: Resultsmentioning

confidence: 94%

Simultaneous Determination of Triterpenoid Saponins from Pulsatilla koreana using High Performance Liquid Chromatography Coupled with a Charged Aerosol Detector (HPLC-CAD)

Yeom¹,

Suh²,

Youm³

et al. 2010

Bulletin of the Korean Chemical Society

View full text Add to dashboard Cite

Several triterpenoid saponins from root of Pulsatilla koreana Nakai (Ranunculaceae) were studied and their biological activities were reported. It is difficult to analyze triterpenoid saponins using HPLC-UV due to the lack of chromophores. So, evaporative light scattering detection (ELSD) is used as a valuable alternative to UV detection. More recently, a charged aerosol detection (CAD) has been developed to improve the sensitivity and reproducibility of ELSD. In this study, we developed and validated a novel method of high performance liquid chromatography coupled with a charged aerosol detector for the simultaneous determination of four triterpenoid saponins: pulsatilloside E, pulsatilla saponin H, anemoside B4 and cussosaponin C. Analytes were separated by the Supelco Ascentis ® Express C18 column (4.6 mm × 150 mm, 2.7 µm) with gradient elution of methanol and water at a flow rate of 0.8 mL/min at 30 o C. We examined various factors that could affect the sensitivity of the detectors, including various concentrations of additives, the pH of the mobile phase, and the CAD range. Linear calibration curves were obtained within the concentration ranges of 2 -200 µg/mL for pulsatilloside E, anemoside B4 and cussosaponin C, and 5 -500 µg/mL for pulsatilla saponin H with correlation coefficient (R 2 ) greater than 0.995. The limit of detection (LOD) and quantification (LOQ) were 0.04 -0.2 and 2 -5 µg/mL, respectively. The validity of the developed HPLC-CAD method was confirmed by satisfactory values of linearity, intra-and inter-day accuracy and precision. This method could be successfully applied to quality evaluation, quality control and monitoring of Pulsatilla koreana.

show abstract

“…Shell particles are a relatively recent trend in chromatographic separation, but several pharmaceutical-bioanalytical [105][106][107][108][109][110][111][112][113][114][115][116][117][118], food analytical [119][120][121][122][123][124][125][126][127][128][129], environmental [130][131][132][133][134] and multidimensional [135][136][137] separations can be found in the literature. Very recently, new commercially available wide-pore stationary phases demonstrate exceptional efficiency for protein separations [138][139][140].…”

Section: Superficially Porous Particlesmentioning

confidence: 99%

Evolution and Current Trends in Liquid and Supercritical Fluid Chromatography

Fekete¹,

Perrenoud²,

Guillarme

2014

CCHG

View full text Add to dashboard Cite

Abstract:The current trend in high performance liquid chromatography (HPLC) tends toward the achievement of higher separation efficiency and shorter analysis time. Indeed, better performance in LC has become increasingly important in recent years mainly driven by the challenges of either analyzing more complex samples or increasing the numbers of samples per time unit. In the recent development of particle technology, the use of fully porous sub-2 m particles and sub-3 m shell particles have received considerable attention. Beside packed columns, the new generation of silica-based monolithic columns also offers very high separation power. However, to take full advantage of these innovative phases, the chromatographic system has also to be drastically optimized in terms of upper pressure limit and system volume.This revolution in column technology now spreads and covers several modes of liquid chromatography such as reversedphase liquid chromatography (RPLC), hydrophilic interaction liquid chromatography (HILIC), or even supercritical fluid chromatography (SFC). The HILIC and SFC, which can be considered as alternative modes of chromatography, could also be useful to extend the applicability of chromatography towards the analysis of very hydrophilic and lipophilic compounds, respectively.The present review gives an insight about the theory behind the success of current column technology and presents a summary of latest applications, using various modes of one-dimensional chromatography (RPLC, HILIC, SFC). This paper also shows that theoretically expected column efficiency could sometimes be compromised in practical work especially in the case of narrow bore columns.

show abstract

Comparison of fused-core and conventional particle size columns by LC–MS/MS and UV: Application to pharmacokinetic study

Cited by 48 publications

References 33 publications

Pre-Validation and Performance Prediction Using Pressure Monitoring to Evaluate HPLC Method Development Changes

Pre-Validation and Performance Prediction Using Pressure Monitoring to Evaluate HPLC Method Development Changes

Simultaneous Determination of Triterpenoid Saponins from Pulsatilla koreana using High Performance Liquid Chromatography Coupled with a Charged Aerosol Detector (HPLC-CAD)

Evolution and Current Trends in Liquid and Supercritical Fluid Chromatography

Contact Info

Product

Resources

About