Comparison of Franz cells and microdialysis for assessing salicylic acid penetration through human skin

Lévêque, Nathalie; Makki, S.; Hadgraft, Jonathan; Humbert, P

doi:10.1016/j.ijpharm.2003.09.012

Cited by 41 publications

(29 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In these cases, cutaneous microdialysis enables characterization of skin absorption and clearance of drugs from topical formulations (Shinkai et al, 2011;Tang et al, 2013). Leveque et al (2004) studied the skin absorption of salicylic acid by using cutaneous microdialysis and compared the results with those from in vitro skin permeation studies using a Franz diffusion cell (Leveque et al, 2004). Even though the major salicylic acid levels were determined by using the microdialysis technique, the results showed good correlation.…”

Section: Cutaneous Microdialysismentioning

confidence: 99%

Section: In Vitro Evaluation Of the Skin Permeation Of Drugsmentioning

confidence: 99%

“…The first work in this field was developed by Thomas J. Franz in 1970. The basic configuration of the experimental apparatus is composed of a) the donor compartment, wherein the formulation is applied to a semipermeable membrane where the drug released must permeate, and b) a receiver chamber, wherein samples can be withdrawn for drug analysis (Moser et al, 2001;Leveque et al, 2004;Hanson, 2010).The vertical diffusion cell is a modification of the original Franz diffusion cell. This apparatus has been used to evaluate different formulations, such as gels, creams, ointments, and patches (Thakker, Chern, 2003;Hanson, 2010).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of skin absorption of drugs from topical and transdermal formulations

Ruela

Perissinato

Lino

et al. 2016

Braz. J. Pharm. Sci.

207

135

View full text Add to dashboard Cite

The skin barrier function has been attributed to the stratum corneum and represents a major challenge in clinical practice pertaining to cutaneous administration of drugs. Despite this, a large number of bioactive compounds have been successfully administered via cutaneous administration because of advances in the design of topical and transdermal formulations. In vitro and in vivo evaluations of these novel drug delivery systems are necessary to characterize their quality and efficacy. This review covers the most well-known methods for assessing the cutaneous absorption of drugs as an auxiliary tool for pharmaceutical formulation scientists in the design of drug delivery systems. In vitro methods as skin permeation assays using Franz-type diffusion cells, cutaneous retention and tape-stripping methods to study the cutaneous penetration of drugs, and in vivo evaluations as pre-clinical pharmacokinetic studies in animal models are discussed. Alternative approaches to cutaneous microdialysis are also covered. Recent advances in research on skin absorption of drugs and the effect of skin absorption enhancers, as investigated using confocal laser scanning microscopy, Raman confocal microscopy, and attenuated total reflectance Fourier-transform infrared spectroscopy, are reviewed. Uniterms:Skin absorption/effects/study. Skin absorption/topical formulations. Skin absorption/ transdermal formulations. Skin absorptions/enhancers. INTRODUCTIONTopical and transdermal drug delivery systems have shown significant advantages in clinical practice for drug targeting to the action site in the body; this has reduced the systemic side effects. The administration of drugs by through the skin is also performed to achieve controlled or prolonged drug delivery, and this route can be explored as an alternative to the oral route. The oral route shows some limitations for drugs with irregular absorption in the gastrointestinal tract and low bioavailability and for drugs with increased first pass metabolism and short plasma halflife times (Barry, 2001;Wokovich et al., 2006;Prausnitz, Langer, 2009;Alexander et al., 2012).Many drug products applied to the skin surface may penetrate to some extent into the skin layers, where their effects are expected. This is the case for topical formulations for treatment of skin disorders such as acne and cutaneous inflammatory diseases that include dermatitis, erythematous lupus, and psoriasis. On the other hand, transdermal formulations release drugs that permeate through the skin and enter the systemic circulation. Transdermal therapy must ensure that significant concentrations of the drug are absorbed to reach effective plasma concentrations. Permeation of drugs is targeted in some cases to body regions close to the action site, where a regional effect is expected, e.g., in the muscles, blood vessels, and articulations. In this way, the term "cutaneous absorption" is properly used to characterize the sum of the amounts of drug that penetrate and permeate the skin (Barry, 2001;El Maghraby, Barry;Willi...

show abstract

Section: Cutaneous Microdialysismentioning

confidence: 99%

Section: In Vitro Evaluation Of the Skin Permeation Of Drugsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of skin absorption of drugs from topical and transdermal formulations

Ruela

Perissinato

Lino

et al. 2016

Braz. J. Pharm. Sci.

207

135

View full text Add to dashboard Cite

show abstract

“…Thus, this study was undertaken to investigate if pathological changes of diabetes skin would affect percutaneous penetration and absorption of glucocorticoids compared to that of normal skin, with the expectation to provide important preclinical data for clinical tests or rational drug administration. It is well established that the quantity of a compound recovered by microdialysis is only a fraction of that present in the tissue (Leveque et al, 2004). The ratio of the concentration of analyte in microdialysate samples relative to the medium surrounding the probe is defined as recovery (Elmquist & Sawchuk, 1997;Sasongko et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

The percutaneous permeability and absorption of dexamethasone esters in diabetic rats: a preliminary study

Song

Xiong

et al. 2013

Drug Delivery

View full text Add to dashboard Cite

To evaluate the influence of diabetes on the permeation of dexamethasone acetate (DA) and dexamethansone sodium phosphate (DSP), the two major dexamethansone esters in clinical practice, when applied percutaneously, histochemical staining was used to determine the skin morphology; improved Franz diffusion cells and microdialysis were used to assess the percutaneous permeation of DA and DSP in normal and diabetic rats. Histopathological examination showed that the epidermal tissue of diabetic rat was much thinner, the epidermal cell layer was less clear and the stratified arrangement of epidemic cell had almost disappeared and progressive atrophy were developed on the subcutaneous fat. In vitro studies showed that the cumulative and the penetrated DSP amount in Group DM were higher. The mean flux value and the mean depositional amount of Group DM were increased significantly compared to those of Group CTL, whereas the amount of DA penetrating was of no difference. Microdialysis indicated that there was no significant difference between Group CTL and Group DM for all the pharmacokinetic parameters of DA. In contrast, the subcutaneous AUC all values and the C max of DSP were significantly increased compared to the control. In conclusion, diabetic rat skin significantly increased the percutaneous permeation of DSP but had no effect on that of DA. It suggests that patients with diabetes should consider the dose of administration when using DA, DSP or other glucocorticoids topically, as different liposolubilities may play some role in the permeability of these compounds via diabetic skin.

show abstract

“…Special device like the Franz Diffusion cell gives in-vitro information about the diffusion of medicinal agent through a selected membrane, either artificial or prepared from the skin of an animal [22] . In-vivo testing would involve the appearance of the medicinal agent, either within the subcutaneous tissue or in the circulation of the animal used for study.…”

Section: Do the Topical Agents Get Across The Skin Surface To Exert Tmentioning

confidence: 99%

How does Traditional Medicine Supplement Current Orthopaedic Practice and Rehabilitation?

Leung

2016

International Journal of Orthopaedics

View full text Add to dashboard Cite

Orthopaedic practice has gained impressive advancements in the past decades and the most important achievements have been made after the two world wars, when the need for injury and fracture treatment was urgent and vast.Orthopaedic experts of today, might not realize that "Bone setting" used to be the main service provided to skeletal injuries and fractures. "Bone setters" have their special techniques and therapeutic agents (mostly topical) to ensure satisfactory results. They also provide various techniques for the control of pain and to facilitate recovering, i.e. rehabilitation.Although the old traditional techniques have been replaced by modern hospital practice, they remain popular outside the hospital and specialist clinics because patients are in favour of additional treatment measures so that pain is better controlled, and healing and recovery could be quicker.This review selected three important areas of Traditional Medicine that should contribute towards modern Orthopaedic Practice and discusses about the scientific evaluations being done to give evidence to those practices. It will be appropriate for the orthopaedic experts to get acquainted with the apparently unscientific traditional practices and consider making use of them under appropriate situations.

show abstract

Comparison of Franz cells and microdialysis for assessing salicylic acid penetration through human skin

Cited by 41 publications

References 11 publications

Evaluation of skin absorption of drugs from topical and transdermal formulations

Evaluation of skin absorption of drugs from topical and transdermal formulations

The percutaneous permeability and absorption of dexamethasone esters in diabetic rats: a preliminary study

How does Traditional Medicine Supplement Current Orthopaedic Practice and Rehabilitation?

Contact Info

Product

Resources

About