Comparison of Four Microbial Enzymes in Clostridia and Bacteroides Isolated from Human Feces

Nakamura, Joe; Kubota, Yorichika; Miyaoka, Masaaki; Saitoh, Toshihiko; Mizuno, Fumio; Benno, Yoshimi

doi:10.1111/j.1348-0421.2002.tb02723.x

Cited by 95 publications

(57 citation statements)

References 14 publications

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“…Bouhnik et al (1996) also observed a decrease in faecal b-glucuronidase activity after supplementation of the diet with a Bifidobacterium strain. This reduced activity may be due to the relative increase in bifidobacteria, as this genus expresses a low b-glucuronidase activity in comparison with other bacterial genera (Nakamura et al, 2002). Contrary to b-glucuronidase, the b-glucosidase activity significantly increased after B. breve administration.…”

Section: Discussionmentioning

confidence: 53%

“…Bifidobacteria and lactobacilli are the most widely studied probiotic genera and have been shown to exert cancer protective effects in vitro and in vivo (Lidbeck et al, 1992;Pool-Zobel et al, 1993). These organisms have low activities of enzymes involved in the conversion of procarcinogens into potentially carcinogenic compounds (Nakamura et al, 2002). Several studies have described a significant reduction in the bacterial enzyme activity after probiotic administration (Goldin et al, 1980;Goldin and Gorbach, 1984;Ling et al, 1994;Bouhnik et al, 1996;Guerin-Danan et al, 1998;Spanhaak et al, 1998;Haberer et al, 2003), whereas other studies did not find significant effects (Marteau et al, 1990;Tannock et al, 2000;Goossens et al, 2003).…”

Section: Introductionmentioning

confidence: 92%

“…and Clostridium sp.) produce high levels of these enzymes (Nakamura et al, 2002). Presumably, the increase in bifidobacteria is accompanied by a decrease in bacterial species with high b-glucuronidase activity.…”

Section: Discussionmentioning

confidence: 99%

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Effect of dietary intervention with different pre- and probiotics on intestinal bacterial enzyme activities

Preter¹,

Raemen²,

Cloetens³

et al. 2007

Eur J Clin Nutr

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Objective: To investigate the influence of different pre-and probiotics on faecal b-glucuronidase and b-glucosidase activity, as one of the claimed beneficial effects of pre-and probiotics is the hypothesis that these substrates are able to reduce the production of toxic and carcinogenic metabolites by suppressing specific enzyme activities in the colon. Setting: Department of Gastrointestinal Research, University Hospital Gasthuisberg, KU Leuven, Belgium. Design and subjects: The effect was evaluated in a randomized, crossover study in 53 healthy volunteers who were randomly assigned to one of five treatment groups. Interventions: At the start and after a 4-week treatment period, the healthy volunteers collected faeces during 72 h. Lactulose and oligofructose-enriched inulin (OF-IN) were chosen as prebiotics, whereas Lactobacillus casei Shirota, Bifidobacterium breve and Saccharomyces boulardii were selected as probiotics. Two synbiotic combinations were evaluated as well. The enzyme activity was assessed spectrophotometricly. Results: Lactulose and OF-IN significantly decreased b-glucuronidase activity, whereas a tendency to a decreased bglucuronidase activity was observed after L. casei Shirota and B. breve intake. To the contrary, B. breve increased b-glucosidase levels. Supplementation with the synbiotic did not appear to be more beneficial than either compound alone. No influence of S. boulardii was noted. Conclusions: Administration of lactulose, OF-IN, L. casei Shirota or B. breve resulted in a decrease of the b-glucuronidase activity, which is considered beneficial for the host.

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Section: Discussionmentioning

confidence: 53%

Section: Introductionmentioning

confidence: 92%

See 1 more Smart Citation

Effect of dietary intervention with different pre- and probiotics on intestinal bacterial enzyme activities

Preter¹,

Raemen²,

Cloetens³

et al. 2007

Eur J Clin Nutr

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show abstract

“…Modulation of intestinal bacterial enzyme activity has been described as one of the mechanisms through which proand prebiotics exert their benefi cial effects [11,12]. Lactobacilli and bifi dobacteria the most widely studied probiotic genera, have low activities of enzymes involved in the conversion of procarcinogens into potentially carcinogenic compounds and show their protective effects in vitro and in vivo [13,14].…”

Section: Discussionmentioning

confidence: 99%

Effect of Lactobacillus plantarum LS/07 on Intestinal Bacterial Enzyme Activities in the Prevention of Cancer, Atherosclerosis and Dysbiosis

et al. 2016

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The effect of the probiotic strain Lactobacillus plantarum LS/07 on intestinal bacterial enzyme activities -β-glucuronidase (β-GLUCUR), β-galactosidase (β-GAL), and β-glucosidase (β-GLU) in the prevention of cancer, atherosclerosis and dysbiosis was investigated. Male Sprague-Dawley rats were randomly divided into 12 experimental groups: C (control group), AT (atherosclerotic group), CC (carcinogenic group), and then each group in combination with antibiotics and probiotics individually and each group in double combination on antibiotic and probiotic. In the control group the β-glucuronidase activity did not change throughout the experiment. High fat diet in the atherosclerotic group signifi cantly increased the activity of β-glucuronidase (p<0.001) and β-glucosidase (p<0.01). Azoxymethane application in the carcinogenic group signifi cantly increased β-glucuronidase (p<0.01), but reduced β-glucosidase (p<0.01). Daily application of probiotics individually and in double combination with antibiotics increased the activity of β-galactosidase, and β-glucosidase, and positively decreased the level of β-glucuronidase. In the control antibiotic group β-glucuronidase was signifi cantly increased (p<0.05), and β-glucosidase decreased (p<0.01) which can be caused by a change of microfl ora in favor of coliform bacteria. These fi nding indicate the positive effects of probiotic Lactobacillus plantarum LS/07 which allows its use in disease prevention in human and veterinary medicine.

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“…Key physiological functions that might be related to cancer risk include control of epithelial cell proliferation and differentiation, production of essential nutrients and/or bioactive food components, prevention of overgrowth of pathogenic organisms, and stimulation of intestinal immunity (Tappenden and Deutsch 2007). Specific strains of bacteria have been implicated in the pathogenesis of cancer, including Bacteroides, Clostridia and Helicobacter pylori (Nakamura et al 2002;Peek and Blaser 2002). Conversely, some strains of bacteria, including Lactobacillus acidophilus and Bifidobacterium longum, have been shown to inhibit experimentally induced colon tumour development (Rowland et al 1996;McCintosh et al 1999).…”

mentioning

confidence: 99%

Effect of horse chestnut and inulin as single supplements or in combination on chemically induced colon cancer in rats

Szabadosová¹,

Hijová²,

Strojný³

et al. 2013

Vet. Med. - Czech

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Natural bioactive supplements have been extensively studied as preventive agents. The aim of this study was to investigate the efficacy of inulin enriched with oligofructose and Hyppocastani extractum siccum as preventive agents of experimentally induced colon carcinogenesis in rats. Colon carcinogenesis was induced by N,N dimethylhydrazine (DMH) in a dose of 21mg/kg body weight s.c., five times at weekly intervals. Sprague Dawley rats (n = 45) were divided into a control group without DMH; control group with injected DMH; group receiving inulin and injected DMH; group receiving Hyppocastani extractum siccum and injected DMH; group receiving inulin and Hyppocastani extractum siccum and injected DMH. The beneficial effects of natural compounds were determined by analysis of caecal parameters such as pH, composition of microflora, activity of bacterial glycolytic enzymes and production of short-chain fatty acids (SCFA). The counts of coliforms were decreased in the groups receiving inulin enriched with oligofructose (P < 0.01), Hyppocastani extractum siccum (P < 0.001) and the combination of these supplements (P < 0.001). The counts of lactobacilli were significantly increased in all experimental groups receiving natural compounds (P < 0.01; P < 0.001). Experimental groups receiving natural compound alone and in combination resulted in a significant decrease in the activity of β-glucuronidase (P < 0.01; P < 0.001). Administration of inulin and Hyppocastani extractum siccum separately significantly increased the concentration of SCFA compared to the control group with DMH. The achieved results indicate the beneficial effect of prebiotics and plant extracts on metabolic processes in the colon and suggest that they could exert a preventive effect on colon carcinogenesis induced by DMH.Keywords: carcinogenesis; prebiotics; plant extract; prevention List of abbreviations DMH = N, N dimethylhydrazine; SCFA = short chain fatty acid; CRC = colorectal cancer; α-GAL = α-galactosidase;

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Comparison of Four Microbial Enzymes in Clostridia and Bacteroides Isolated from Human Feces

Cited by 95 publications

References 14 publications

Effect of dietary intervention with different pre- and probiotics on intestinal bacterial enzyme activities

Effect of dietary intervention with different pre- and probiotics on intestinal bacterial enzyme activities

Effect of Lactobacillus plantarum LS/07 on Intestinal Bacterial Enzyme Activities in the Prevention of Cancer, Atherosclerosis and Dysbiosis

Effect of horse chestnut and inulin as single supplements or in combination on chemically induced colon cancer in rats

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