Comparison of four different methods for detection of biofilm formation by uropathogens

Panda, Pankaj Kumar; Chaudhary, Uma; Dube, Surya Kumar

doi:10.4103/0377-4929.182013

Cited by 46 publications

(40 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Maximum positivity was seen by Microtiter plate method with 58.33% moderate biofilm producers and 3.33% strong biofilm producers whereas tube adherence method showed only 2.33% strong biofilm producers and 45.00% moderate biofilm producers. Similar results were also produced by Mathur et al and Panda et al (19) Also, all the above observers found the biofilm production in one type of strain of bacteria, but in the current study it was proved in five different strains of bacteria.…”

Section: Discussionsupporting

confidence: 91%

Antibiotic Resistance and Biofilm Formation Among Nosocomial Pathogens in a Tertiary Care Hospital

Kaur¹,

Gill²,

Gill³

et al. 2017

jemds

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 91%

Antibiotic Resistance and Biofilm Formation Among Nosocomial Pathogens in a Tertiary Care Hospital

Kaur¹,

Gill²,

Gill³

et al. 2017

jemds

View full text Add to dashboard Cite

show abstract

“…P. aeruginosa PAO1 biofilms were quantified based on the tube method (Panda et al, 2016). Overnight, P. aeruginosa cultures were diluted 1:50 into 200 μL of Trypticase Soytone broth (TSB, 1.7% tryptone, 0.3% soy protone, 0.25% glucose, 0.5% NaCl, 0.25% KH 2 PO 4 , pH 7.2, OD 620 ≈ 0.1; Cycoń et al, 2016) with varying concentrations of extract.…”

Section: Methodsmentioning

confidence: 99%

Anti-Biofilm and Antivirulence Activities of Metabolites from Plectosphaerella cucumerina against Pseudomonas aeruginosa

Zhou

Chen

et al. 2017

Front. Microbiol.

View full text Add to dashboard Cite

This study reported the efficacy of the metabolites of Plectosphaerella cucumerina, one phyllosphere fungus from Orychophragmus violaceus, against Pseudomonas aeruginosa quorum sensing (QS) and QS-regulated biofilms. The minimum inhibitory concentration (MIC) of the ethyl acetate (EtOAc) extract from P. cucumerina against P. aeruginosa PAO1 was 1.25 mg mL−1. At sub-MIC concentrations, P. cucumerina extract (0.25–1 mg mL−1) not only inhibited biofilm formation but also disrupted preformed biofilms of P. aeruginosa PAO1 without affecting its growth. Fluorescence and scanning electron microscope (SEM) showed architectural disruption of the biofilms when treated with P. cucumerina metabolites. Further investigation demonstrated that metabolites in P. cucumerina attenuated the QS-dependent virulence factors. LC-MS/MS spectra coupled with experimentally standard samples suggested that patulin and emodin might act as the principal components possessing anti-biofilm and antivirulence activities. This is the first report of (1) the isolation of P. cucumerina from the phyllosphere of O. violaceus and (2) anti-biofilm, antivirulence, and biofilm disruption activities of this fungus. Thus, this study provides fascinating new pathways for screening antipathogenic agents.

show abstract

“…Optical densities values indicated bacterial adherence to the wells and biofilm formation. The OD values were calculated and biofilm production was graded into strong, moderate and non/weak (Table 1) as described in previous studies (2,(12)(13)(14).…”

Section: Tissue Culture Plate Methodsmentioning

confidence: 99%

“…Bacteria tend to live in a community-like assembly called biofilm. Development of bacterial biofilms occurs in a dynamic process that includes bacterial attachment to a particular surface, irreversible binding and formation of a hydrated matrix of polysaccharides and protein (1,2). Surfaces that favor biofilm development include inert surfaces as medical devices and dead tissues as dead bone fragments (1,3).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Tube method and Congo red agar versus tissue culture plate method for detection of biofilm production by uropathogens isolated from midstream urine: Which one could be better?

Sultan¹,

Nabiel²

2018

Af J Clin Exp Micro

View full text Add to dashboard Cite

Background: Bacteria tend to live in assemblies called biofilms that aid bacterial virulence. Biofilms contribute to the development of antibiotic resistant urinary tract infection. Therefore, detection of biofilm production by urinary pathogens can assist the physicians to initiate the proper antimicrobial treatment. Methods:We conducted a prospective study that included patients with suspected urinary tract infection. Collected midstream urine samples were processed by standard microbiological techniques. Detection of biofilm production by the isolated uropathogens was conducted by tissue culture plate method (TCPM), tube method (TM) and Congo red agar (CRA). Results:A total of 43 (29.7%) isolated uropathogens showed positive biofilm formation by TCPM which was considered the gold standard for biofilm detection. When compared with the TCPM, TM truly identified 40 biofilm producers and 83 non-biofilm producers showing sensitivity and specificity of 93.0% and 81.4% respectively. The CRA truly identified 38 biofilm producers and 77 non-biofilm producers with sensitivity and specificity of 88.4% and 75.5% respectively. Conclusion:The TM was superior to CRA in biofilm detection and demonstrated better sensitivity and specificity results. Out of the investigated three phenotypic biofilm detection methods, the TCPM was the ideal method for detection of biofilm formation by uropathogens isolated from midstream urine samples. It can be used routinely in the microbiology laboratory with good specificity results and less subjectivity errors. ABSTRAIT Contexte: Les bactéries ont tendance à vivre dans des assemblages appelés biofilms qui facilitent la virulence bactérienne. Les biofilms contribuent au développement d'une infection des voies urinaires résistante aux antibiotiques. Nous avons détecté des uropathogènes produisant des biofilms isolés à partir de l'urine médiane et évalué trois méthodes phénotypiques in vitro pouvant être appliquées en laboratoire pour la détection de biofilms.L'ARC a vraiment identifié 38 producteurs de biofilms et 77 producteurs de non-biofilms avec une sensibilité et une spécificité de 88,4% et 75,5% respectivement.Conclusion: La MT était supérieure à l'ARC pour la détection de biofilm et a démontré de meilleurs résultats de sensibilité et de spécificité. Parmi les trois méthodes de détection phénotypiques de biofilms étudiées, le TCPM était la méthode idéale pour détecter la formation de biofilm par des uropthogènes isolés à partir d'échantillons d'urine en cours de route. Il peut être utilisé en routine dans le laboratoire de microbiologie avec de bons résultats de spécificité et moins d'erreurs de subjectivité.

show abstract

Comparison of four different methods for detection of biofilm formation by uropathogens

Abstract: TCP method is the ideal method for detection of bacterial biofilm formation by uropathogens. MCRA method is superior only to CRA for detection of staphylococcal biofilm formation.

Cited by 46 publications

References 0 publications

Antibiotic Resistance and Biofilm Formation Among Nosocomial Pathogens in a Tertiary Care Hospital

Antibiotic Resistance and Biofilm Formation Among Nosocomial Pathogens in a Tertiary Care Hospital

Anti-Biofilm and Antivirulence Activities of Metabolites from Plectosphaerella cucumerina against Pseudomonas aeruginosa

Tube method and Congo red agar versus tissue culture plate method for detection of biofilm production by uropathogens isolated from midstream urine: Which one could be better?

Contact Info

Product

Resources

About