Comparison of Four Commercially Available Assays for the Detection of IgM Phase II Antibodies to <i>Coxiella burnetii</i> in the Diagnosis of Acute Q Fever

Frangoulidis, Dimitrios; Schröpfer, Elmar; Dahouk, Sascha Al; Tomaso, Herbert; Meyer, Hermann

doi:10.1196/annals.1374.110

Cited by 8 publications

(7 citation statements)

References 3 publications

(6 reference statements)

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“…The present study clearly shows that ELISAs have an important role in Q fever diagnostics, but they are not only limited in the distinction between acute, chronic, and past disease but also show large differences in sensitivity and specificity. Both our study and other studies demonstrate that the IFAT remains the most reliable test system for the serological diagnosis of Q fever (9,(12)(13)(14)(15).…”

Section: Discussionsupporting

confidence: 76%

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Comparison of Different Commercially Available Enzyme-Linked Immunosorbent Assays with Immunofluorescence Test for Detection of Phase II IgG and IgM Antibodies to Coxiella burnetii

Dangel¹,

Koempf²,

Fischer³

2020

J Clin Microbiol

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Several commercially available enzyme-linked immunosorbent assays (ELISAs) for the detection of phase II IgG or IgM antibodies against Coxiella burnetii were compared. In addition, an indirect immunofluorescence test was used as a confirmation test. In all, 70 serum samples for IgG and 43 serum samples for IgM were tested. The ELISAs showed large differences in sensitivity and specificity, which led to a partially high ratio of false-negative determinations. The most convincing test was PanBio from Abbott, which unfortunately can only test IgG but not IgM.

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Section: Discussionsupporting

confidence: 76%

“…For both systems, different commercial kits are available; these need to be tested and compared occasionally. In the 1990s and 2000s, several laboratories tested diverse ELISAs and IFATs mainly focused on the detection of phase II IgM antibody titers (12)(13)(14)(15).…”

mentioning

confidence: 99%

Comparison of Different Commercially Available Enzyme-Linked Immunosorbent Assays with Immunofluorescence Test for Detection of Phase II IgG and IgM Antibodies to Coxiella burnetii

Dangel¹,

Koempf²,

Fischer³

2020

J Clin Microbiol

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“…A population-based survey or follow up of patients to obtain a convalescent sera sample was not feasible due to the nomadic lifestyle of the patients, the ongoing inter-clans conflicts and militia activities in the region. However previous studies have reported adequate performance of the used commercial serion ELISA classic test kits in detection of phase II specific IgG and IgM [ 36 , 62 ] respectively, in diagnosis of acute Q fever. The novelty of the present study was that the ELISA positive sera were further confirmed with the reference diagnostic method (IFA) and the patients whose fever onset was ≤ 2 weeks were further screened for Q fever using a quantitative real-time PCR.…”

Section: Discussionmentioning

confidence: 99%

Febrile patients admitted to remote hospitals in Northeastern Kenya: seroprevalence, risk factors and a clinical prediction tool for Q-Fever

et al. 2016

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BackgroundQ fever in Kenya is poorly reported and its surveillance is highly neglected. Standard empiric treatment for febrile patients admitted to hospitals is antimalarials or penicillin-based antibiotics, which have no activity against Coxiella burnetii. This study aimed to assess the seroprevalence and the predisposing risk factors for Q fever infection in febrile patients from a pastoralist population, and derive a model for clinical prediction of febrile patients with acute Q fever.MethodsEpidemiological and clinical data were obtained from 1067 patients from Northeastern Kenya and their sera tested for IgG antibodies against Coxiella burnetii antigens by enzyme-linked-immunosorbent assay (ELISA), indirect immunofluorescence assay (IFA) and quantitative real-time PCR (qPCR). Logit models were built for risk factor analysis, and diagnostic prediction score generated and validated in two separate cohorts of patients.ResultsOverall 204 (19.1 %, 95 % CI: 16.8–21.6) sera were positive for IgG antibodies against phase I and/or phase II antigens or Coxiella burnetii IS1111 by qPCR. Acute Q fever was established in 173 (16.2 %, 95 % CI: 14.1–18.7) patients. Q fever was not suspected by the treating clinicians in any of those patients, instead working diagnosis was fever of unknown origin or common tropical fevers. Exposure to cattle (adjusted odds ratio [aOR]: 2.09, 95 % CI: 1.73–5.98), goats (aOR: 3.74, 95 % CI: 2.52–9.40), and animal slaughter (aOR: 1.78, 95 % CI: 1.09–2.91) were significant risk factors. Consumption of unpasteurized cattle milk (aOR: 2.49, 95 % CI: 1.48–4.21) and locally fermented milk products (aOR: 1.66, 95 % CI: 1.19–4.37) were dietary factors associated with seropositivity. Based on regression coefficients, we calculated a diagnostic score with a sensitivity 93.1 % and specificity 76.1 % at cut off value of 2.90: fever >14 days (+3.6), abdominal pain (+0.8), respiratory tract infection (+1.0) and diarrhoea (−1.1).ConclusionQ fever is common in febrile Kenyan patients but underappreciated as a cause of community-acquired febrile illness. The utility of Q fever score and screening patients for the risky social-economic and dietary practices can provide a valuable tool to clinicians in identifying patients to strongly consider for detailed Q fever investigation and follow up on admission, and making therapeutic decisions.

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“…In human studies, the ELISA also has a high sensitivity and good specificity. For example, in the diagnosis of acute Q‐fever in man, the ELISA has a reported sensitivity of 80% for anti‐phase II IgG and 84% for anti‐phase II IgM and a specificity of >99% in one study (Waag et al., ), and in a other study a specificity of 97.7% and sensitivity of 95.65% for anti‐phase II IgM (Frangoulidis et al., ).…”

Section: Discussionmentioning

confidence: 99%

Coxiella burnetii(Q-Fever) Seroprevalence in Prey and Predators in the United Kingdom: Evaluation of Infection in Wild Rodents, Foxes and Domestic Cats Using a Modified ELISA

Meredith

Cleaveland

Denwood

et al. 2014

Transbound Emerg Dis

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Comparison of Four Commercially Available Assays for the Detection of IgM Phase II Antibodies to Coxiella burnetii in the Diagnosis of Acute Q Fever

Abstract: Four commercially available serological assays for the detection of IgM phase II antibodies in patients with acute Q fever infection were compared using a panel of 23 serum samples from patients with acute Q fever and 88 control sera from blood donors.

Cited by 8 publications

References 3 publications

Comparison of Different Commercially Available Enzyme-Linked Immunosorbent Assays with Immunofluorescence Test for Detection of Phase II IgG and IgM Antibodies to Coxiella burnetii

Comparison of Different Commercially Available Enzyme-Linked Immunosorbent Assays with Immunofluorescence Test for Detection of Phase II IgG and IgM Antibodies to Coxiella burnetii

Febrile patients admitted to remote hospitals in Northeastern Kenya: seroprevalence, risk factors and a clinical prediction tool for Q-Fever

Coxiella burnetii(Q-Fever) Seroprevalence in Prey and Predators in the United Kingdom: Evaluation of Infection in Wild Rodents, Foxes and Domestic Cats Using a Modified ELISA

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