Comparison of four commercial RT‐PCR diagnostic kits for COVID‐19 in China

Shen, Lingyu; Cui, Shujuan; Zhang, Daitao; Lin, Changying; Chen, Lijuan; Wang, Quanyi

doi:10.1002/jcla.23605

Cited by 15 publications

(8 citation statements)

References 21 publications

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“…The authors conclude that this is likely the result of the use of half the elution volume and thus a higher RNA concentration. Also, there are similar studies by van Kasteren et al (35) and Shen et al (36), but about the evaluation of diagnostic kits. Very few studies have carried out this type of evaluation, and it is impossible to compare the results of this study directly.…”

Section: Evaluation Of Performance Characteristics Of Kitsmentioning

confidence: 66%

Investigation of SARS-CoV-2 Detection Method Applicability and Virus Occurrence in Food and Food Packaging

Mlinar,

Kostelac,

Kovaček

et al. 2023

Food Technol. Biotechnol. (Online)

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Research background. While it is clear that SARS CoV-2 coronavirus is the primary respiratory virus, there are no entirely clarified ways of transmission. Foodborne transmission remained an unexplained path of transmission. Therefore, the goals of this paper are to examine and present an assessment of the most appropriate of the four selected kits for RNA extraction for the testing and detection of SARS-CoV-2 on food packaging and food surfaces. After this, it could be possible to indicate the possibility of infection through contact or direct food consumption. Experimental approach. Finding the best technique is vital as RNA extraction is one of the essential elements in detecting SARS-CoV-2. That was achieved through an experiment on four commercial kits with the original manufacturer protocol and with modification applications of ethanol and isopropanol. The selected kit was used for RNA extraction from the swab and the food samples. The coronavirus was then identified using real-time reverse transcription-polymerase chain reaction (RT-PCR) assays to determine whether the SARS-CoV-2 virus or viral particles are present in the food chain with the overall purpose of demonstrating the possibility that food can contribute as a vehicle for the transmission of the virus. Results and conclusions. The findings of this investigation made the most effective extraction kit and protocol stand out. The results of applicability confirmation of the kit indicated a significant share of positive samples on viral SARS-CoV-2 virus particles on surfaces. However, according to the second part of the study's findings, the virus was not detected in examined samples of food packaging surfaces, food surfaces, and in food. Novelty and scientific contribution. The study and presented results defined one of the most suitable protocols for isolating RNA from environmental surface samples. The main contribution of the study is in the presentation of the results of the study, that is, the examination of samples that are primarily related to the food chain, food packaging, food surfaces, and the food itself in the sense of ready-to-eat food. The results of this study could also be helpful for further determining the potential of food as a vector for the transmission of coronaviruses.

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Section: Evaluation Of Performance Characteristics Of Kitsmentioning

confidence: 66%

Investigation of SARS-CoV-2 Detection Method Applicability and Virus Occurrence in Food and Food Packaging

Mlinar,

Kostelac,

Kovaček

et al. 2023

Food Technol. Biotechnol. (Online)

View full text Add to dashboard Cite

show abstract

“…A number of studies have independently evaluated SARS-CoV-2 diagnostic kits with various performance outcomes ( 12 – 17 ). In this study, we evaluated the analytical performance of four standalone SARS-COV-2 RT-PCR kits that are available in South Africa, using a concise testing panel.…”

Section: Discussionmentioning

confidence: 99%

Performance Evaluation of Four Qualitative RT-PCR Assays for the Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)

et al. 2023

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“…Despite the presence of many serum-based laboratory tests, antibody detection serological tests and many real-time PCR-based assays, most still have drawbacks. Moreover, there is great variability in the sensitivity and specificity of existing PCR-based tests, depending on the vendor, the strain, the viral load and the location at which the swab is used [43,44]. Additionally, PCR results should be correlated with patient history and symptoms for infection confirmation; for instance, a negative COVID-19 PCR test does not exclude infection for the above-mentioned reasons.…”

Section: Discussionmentioning

confidence: 99%

In Silico Identification and Clinical Validation of a Novel Long Non-Coding RNA/mRNA/miRNA Molecular Network for Potential Biomarkers for Discriminating SARS CoV-2 Infection Severity

Agwa

Elghazaly

Meteini

et al. 2021

Cells

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(1) Background: The coronavirus (COVID-19) pandemic is still a major global health problem, despite the development of several vaccines and diagnostic assays. Moreover, the broad symptoms, from none to severe pneumonia, and the various responses to vaccines and the assays, make infection control challenging. Therefore, there is an urgent need to develop non-invasive biomarkers to quickly determine the infection severity. Circulating RNAs have been proven to be potential biomarkers for a variety of diseases, including infectious ones. This study aimed to develop a genetic network related to cytokines, with clinical validation for early infection severity prediction. (2) Methods: Extensive analyses of in silico data have established a novel IL11RA molecular network (IL11RNA mRNA, LncRNAs RP11-773H22.4 and hsa-miR-4257). We used different databases to confirm its validity. The differential expression within the retrieved network was clinically validated using quantitative RT-PCR, along with routine assessment diagnostic markers (CRP, LDH, D-dimmer, procalcitonin, Ferritin), in100 infected subjects (mild and severe cases) and 100 healthy volunteers. (3) Results: IL11RNA mRNA and LncRNA RP11-773H22.4, and the IL11RA protein, were significantly upregulated, and there was concomitant downregulation of hsa-miR-4257, in infected patients, compared to the healthy controls, in concordance with the infection severity. (4) Conclusion: The in-silico data and clinical validation led to the identification of a potential RNA/protein signature network for novel predictive biomarkers, which is in agreement with ferritin and procalcitonin for determination of COVID-19 severity.

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Comparison of four commercial RT‐PCR diagnostic kits for COVID‐19 in China

Cited by 15 publications

References 21 publications

Investigation of SARS-CoV-2 Detection Method Applicability and Virus Occurrence in Food and Food Packaging

Investigation of SARS-CoV-2 Detection Method Applicability and Virus Occurrence in Food and Food Packaging

Performance Evaluation of Four Qualitative RT-PCR Assays for the Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)

In Silico Identification and Clinical Validation of a Novel Long Non-Coding RNA/mRNA/miRNA Molecular Network for Potential Biomarkers for Discriminating SARS CoV-2 Infection Severity

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