Comparison of Four Chromogenic Media and Hektoen Agar for Detection and Presumptive Identification of
            <i>Salmonella</i>
            Strains in Human Stools

Pérez, Jose Díaz; Cavalli, P.; Roure, C.; Renac, Rodolphe; Gille, Y.; Freydière, Anne-Marie

doi:10.1128/jcm.41.3.1130-1134.2003

Cited by 57 publications

(39 citation statements)

References 28 publications

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“…A number of different types of selective media have been developed to enhance the recovery of enteric pathogens from stool samples. Unfortunately, these media have been shown to have poor specificity and produce numerous false-positive colonies (11,16,33). The identification and confirmation of suspicious colonies is highly nonspecific, and it places a heavy burden on the laboratory in terms of biochemical and serological testing (11,16,34).…”

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confidence: 99%

Mass Spectrometry Biotyper System Identifies Enteric Bacterial Pathogens Directly from Colonies Grown on Selective Stool Culture Media

et al. 2010

J Clin Microbiol

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We evaluated the performance and cost-effectiveness of a matrix-assisted laser desorption ionization timeof-flight mass spectrometry-based Biotyper system for the routine identification of common enteric bacterial pathogens seen in middle Tennessee from suspicious colonies grown on selective stool culture media. A total of 304 suspicious colonies were selected and further identified from 605 stool specimens. The suspicious colonies were analyzed by the Biotyper system, and the results were compared to those from routine phenotypic methods, which identified 22 Salmonella species, 39 Shigella species, 3 enterohemorrhagic Escherichia coli (EHEC) isolates, 2 Yersinia enterocolitica isolates, 2 Campylobacter species, and 236 gastrointestinal normal flora isolates. The Biotyper system correctly identified the Salmonella species, Yersinia enterocolitica, and Campylobacter species but failed to distinguish the Shigella species and EHEC isolates from E. coli. Among the 236 normal flora isolates, 233 (98.7%) and 228 (96.6%) agreed at the genus and species levels, respectively, between the phenotypic and Biotyper methods. Organism identification scores were insignificantly different between colonies directly from selective media and subsequently from pure subculture. The entire Biotyper identification procedure, from smear preparation to final result reporting, can be completed within 30 min. The Biotyper system provides a rapid and simple screening tool for identifying many, but not all, suspicious colonies grown on selective media within 24 h after inoculation, which shortens test turnaround time by 2 to 3 days.

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confidence: 99%

Mass Spectrometry Biotyper System Identifies Enteric Bacterial Pathogens Directly from Colonies Grown on Selective Stool Culture Media

et al. 2010

J Clin Microbiol

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“…MLCB is used principally for Salmonella detection in food and environmental samples, due to its high selectivity (Nye et al, 2002;van Schothorst et al, 1987). On the other hands, XLD is the most commonly used medium for isolating S. enterica (Nye et al, 2002), and CAS is superior to other selective agars for isolating Salmonella from stool samples (Gailot et al, 1999;Maddocks et al, 2002;Perez et al, 2003).…”

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confidence: 99%

Rapid Quantitative Detection of Salmonella enterica Using Fluorescence In Situ Hybridization with Filter-cultivation (FISHFC) Method

Shimizu

Aoi

Osanai

et al. 2013

FSTR

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Specific detection and enumeration of Salmonella enterica in food using conventional culture-based methods (CCBM) are time consuming and labor intensive. This study was conducted to develop a rapid S. enterica detection and enumeration method by combining fluorescence in situ hybridization (FISH) with micro-colony formation culture (FISHFC). Specificity tests of the SAL343 probe for S. enterica detection revealed that SAL343-associated fluorescent micro-colonies were observed specifically for S. enterica, but not for any other organisms. This finding suggests that SAL343 is highly specific for detecting S. enterica using FISHFC. For validation, FISHFC with SAL343 was compared to CCBM, with multiple selective agar, using spiked food samples; no significant differences in enumeration were found between FISHFC and CCBM (p > 0.05). The FISHFC method allowed enumeration of S. enterica within 10 h while CCBM allowed enumeration within 5 days. Therefore, the FISHFC method has potential application for more rapid and specific enumeration of S. enterica in food samples compared to other available methods.Keywords: Salmonella enterica, fluorescence in situ hybridization, FISHFC, rapid detection *To whom correspondence should be addressed. E-mail: yamasaki@fish.hokudai.ac.jp IntroductionSalmonella are Gram-negative, facultatively anaerobic, rod-shaped bacteria that exist ubiquitously in the environment and are commonly found in water, animal intestine, reptilian skin and food. Salmonella also causes salmonellosis in humans worldwide due to the consumption of contaminated food such as fresh fruits and vegetables (Matthews, 2006). The genus Salmonella currently consists of two species: S. enterica and S. bongori. S. enterica is comprised of six subspecies: enterica, salamae, arizonae, diarizonae, houtenae and indica, which are further subdivided into more than 2,500 serotypes (Agbaja et al., 2011;Su and Chiu, 2007). Most of the Salmonella responsible for disease in humans and animals are members of S. enterica. S. enterica serotype Enteritidis and Typhimurium are two of the main sources of human food poisoning. Although S. bongori has been reported to infect humans, it is generally associated with cold-blooded animals (Giammanco et al., 2002). Therefore, S. enterica are clinically important pathogens of humans.Routine methods for enumerating Salmonella are traditional microbiological methods based on plating using selective agar and biochemical identification. However, the biochemical characteristics of some isolates may differ from the common phenotypic properties of typical strains. Moreover, it is recommended that multiple selective agars be used for Salmonella detection in food samples (Nye et al., 2002). Thus, the conventional methods for detecting Salmonella are time-consuming and labor-intensive, taking at least several days to obtain the final results (Kim et al., 2007). To overcome technical problems associated with Salmonella detection, a number of molecular-based methods, such as PCR, quantitative PCR (qPCR), enzy...

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“…Biz çalışmamızda klasik besiyerleri ile daha önce karşılaş-tırma çalışmaları yapılmış, güvenilirliğini kanıtlamış bir kromojenik besiyeri olan CHROMagar Salmonella plus besiyerini kullanmayı tercih ettik [23][24][25][26] . Salmonella türü bakterilerin bu besiyerinde yaptıkları tipik koloni görünümü ve rengi (S. Typhi, S. Paratyphi A ve laktoz pozitif Salmonella bu besiyerinde pembe/leylak rengi olarak görülür) nedeni ile ayrıca zaman kaybettirici saflaştırma işlemlerine gerek kalmamaktadır 6 .…”

Section: Maldi-tof'un Başarısı Phoenix Otomatize Cihazı Ileunclassified

Salmonella Türlerinin Hızlı Tanısında Kromojenik Besiyeri İle Birlikte “Matrix-Assisted Laser Desorption Ionization - Time of Flight Mass Spectrometry” Yönteminin Phoenix Otomatize Sistemi ile Kıyaslanması

Akyar¹,

Can²

2013

Kafkas Univ Vet Fak Derg

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Comparison of Four Chromogenic Media and Hektoen Agar for Detection and Presumptive Identification of Salmonella Strains in Human Stools

Cited by 57 publications

References 28 publications

Mass Spectrometry Biotyper System Identifies Enteric Bacterial Pathogens Directly from Colonies Grown on Selective Stool Culture Media

Mass Spectrometry Biotyper System Identifies Enteric Bacterial Pathogens Directly from Colonies Grown on Selective Stool Culture Media

Rapid Quantitative Detection of Salmonella enterica Using Fluorescence In Situ Hybridization with Filter-cultivation (FISHFC) Method

Salmonella Türlerinin Hızlı Tanısında Kromojenik Besiyeri İle Birlikte “Matrix-Assisted Laser Desorption Ionization - Time of Flight Mass Spectrometry” Yönteminin Phoenix Otomatize Sistemi ile Kıyaslanması

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