Comparison of four antibiotics for inactivating leptospires in bull semen diluted in egg yolk extender and experimentally inoculated with Leptospira santarosai serovar guaricura

Miraglia, Fabiana; Morais, Zenáide Maria de; Cortéz, Adriana; Melville, Priscilla Anne; Marvullo, Maria Fernanda Vianna; Richtzenhain, Leonardo José; Visintin, José Antônio; Vasconcellos, Sílvio Arruda

doi:10.1590/s1517-83822003000200011

Cited by 7 publications

(6 citation statements)

References 18 publications

(12 reference statements)

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“…There was a positive correlation (P<0.05) between culture positive and PCR positive from renal tissue, determined by Mc Nemar's test (55). As expected, PCR analysis was more sensitive (11/137) than culture (4/137), similar to what was found by other authors (11,37,52,53). The analysis of agreement adjusted (kappa indicator) (58), when it was compared the direct methods of leptospire´s research (PCR e cultivo), showed low agreement between PCR and culture from kidney (k=0.27), liver (k=0.00), reproductive tract (k = -48.65) and urine (k=0.00).…”

Section: Resultssupporting

confidence: 91%

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Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil

Miraglia¹,

Moreno²,

Gomes³

et al. 2008

Braz. J. Microbiol.

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With the aim of isolating Leptospira spp., blood serum, kidney, liver and genital tract of 137 female swine (40 sows and 97 gilts) and also urine samples from 22 sows were collected in a slaughterhouse in the State of São Paulo, from April 2003 to August 2004. Four isolates were obtained from animals that presented microagglutination test (MAT) titers ≥ 100 for the serovar Pomona and one was obtained from an animal negative by MAT in which Leptospira was isolated from the liver and reproductive tract. The presence of leptospiral DNA was investigated by PCR, and positive results were found in kidneys of 11 females, liver of two, genital tract of two and urine of one of them. Nephrosis, interstitial multifocal nephritis, moderate to severe changing, hyalines cylinders and hemorrhagic focuses, hepatic and uterine horns congestion were histological lesions observed in higher frequency in animals positive for leptospira. The silver impregnation (Warthin Starry) confirmed the presence of spirochetes in renal tubules of four females with positive leptospira cultures from kidneys. The serogroup of the five isolates was identified as Pomona by cross agglutination with reference polyclonal antibodies. Molecular characterization of the isolates was carried out by variablenumber tandem-repeats analysis. All the isolates revealed a pattern distinct from the L. interrogans Pomona type strain, but identical to a previously identified pattern from strains isolated in Argentina belonging to serovar Pomona.

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Section: Resultssupporting

confidence: 91%

“…Samples of 10 g of kidneys, liver, uterus, oviducts and ovaries were collected and homogenized in 50 mL of Sorensen saline (48 (48), were inoculated into culture tubes in duplicate (37). The cultures were checked once a week over 4-6 months.…”

Section: Isolation Of Leptospiresmentioning

confidence: 99%

Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil

Miraglia¹,

Moreno²,

Gomes³

et al. 2008

Braz. J. Microbiol.

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“…For isolation, 5 g of kidney (7) or abortion samples (2) were collected and homogenized in 50 mL of Sorensen saline; 100 µL of 10 -1 , 10 -2 and 10 -3 dilutions were inoculated into culture tubes in duplicate containing modified EMJH medium (Difco-BBL, Detroit /USA) enriched with 15% rabbit serum, 5-fluorouracil and nalidixic acid [13]. Once isolated, cultures were stored in EMJH semi-solid media at 30°C and maintained in EMJH liquid medium until serotyping, microdilution, and molecular analysis.…”

Section: Bacterial Isolates and Culture Conditionsmentioning

confidence: 99%

Characterization of Leptospira interrogans serovar Pomona isolated from swine in Brazil

Miraglia

Moreno

Morais

et al. 2015

J Infect Dev Ctries

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Introduction: Leptospira interrogans swine infection is a cause of serious economic loss and a potential human health hazard. In Brazil, the most common serovars associated with swine infections are Pomona, Icterohaemorrhagie and Tarassovi. Cross-reactions among serovars and the failure of infected animals to seroconvert may complicate the interpretation of serological tests. Molecular methods with better discriminatory powers are useful tools for swine leptospirosis characterization and diagnosis. Methodology: This study evaluated nine L. interrogans isolates from the States of Sao Paulo and Minas Gerais during different time periods.Isolates from diseased and apparently healthy swine were characterized by microscopic agglutination tests with polyclonal antibodies and were genotyped by VNTR, PFGE and MLST techniques. Broth microdilution was used to determine the minimal inhibitory concentration of the antimicrobials of veterinary interest. Results: The strains were identified as L. interrogans serogroup Pomona serovar Pomona Genotype A, while MLST grouped all of the isolates in sequence type 37. The PFGE analysis resulted in two pulsotypes with more than 70% similarity, distinguishing serovar Pomona isolates from the serovar Kennewicki reference strain. All of the isolates presented low MIC values to penicillin, ampicillin, ceftiofur and tulathromycin. High MIC values for fluoroquinolones, tiamulin, gentamicin, tetracyclines, neomycin, tilmicosin and sulfas were also observed. Conclusions: All molecular techniques were concordant in L. interrogans serovar Pomona identification. This serovar may have a different antibiotic susceptibility profile than previously reported for Leptospira isolates.

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“…Quanto aos antibióticos, há relatos na literatura comprovando que as leptospiras podem suportar as concentrações empregadas no processo de congelamento, com ou sem redução em sua virulência (HOAG & BELL, 1955;BRYAN & BOLEY, 1975). MIRAGLIA et al (2003), comparando a capacidade de quatro antibióticos, acrescidos ao diluidor de sêmen gema-citrato, concluiu que a associação penicilina e estreptomicina apresentou os melhores resultados na capacidade de destruir leptospiras, mas houve 2,0% (7/348) de cultivos positivos para leptospiras.…”

Section: Introductionunclassified

Uso De Estreptomicina Na Eliminação Da Leptospirúria Em Touros (Bos Taurus Indicus) Naturalmente Infectados Pelo Sorovar Hardjo

et al. 2005

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RESUMO O objetivo desse trabalho foi verificar a ação de marcas diferentes de estreptomicinas polivalentes para o tratamento da leptospirose bovina em dose única (25 mg de estreptomicina por kg de peso corpóreo). O trabalho foi realizado com 14 touros adultos sorologicamente reagentes para Leptospira interrogans sorovar Hardjo, com título mínimo de 800 e com cultivo positivo. Os animais foram separados em 2 grupos de acordo com a marca da estreptomicina utilizada no tratamento, grupo 1: estreptomicina A e grupo 2: estreptomicina B. Os bovinos controles não receberam nenhum tratamento. Foram obtidas amostras de sangue e urina dos animais tratados e controles nos dias -1, 0, 1, 2, 3, 5, 10, 15 e 25; considerando-se o dia do tratamento como dia 0. Na urina dos bovinos tratados e controle foi realizada a reação em cadeia da polimerase (PCR) e isolamento com inoculação em hamsters. Observou-se que a estreptomicina da marca A, em dose única de 25 mg/kg de peso corpóreo, conseguiu eliminar a leptospirúria no grupo de touros após 24h do tratamento. Já no grupo de touros tratados com a estreptomicina da marca B, foi constatado a leptospirúria entre 48h e 72h, após o tratamento. Em ambos os grupos tratados, a resposta sorológica apresentou uma variação da queda dos níveis dos títulos de anticorpos aglutinantes, sendo que embora a estreptomicina A tenha aparentemente apresentado um melhor desempenho quando comparada com as médias geométricas dos títulos do grupo tratado com a estreptomicina B as médias não diferiram entre si pelo Teste de Tukey (P > 0,05). Nos bovinos tratados a leptospirúria foi intermitente e a média geométrica dos títulos foi menor que a média geométrica dos títulos dos bovinos controles. A diferença do efeito da ação das diferentes marcas de estreptomicina está possivelmente na qualidade da matéria prima produzida pelos laboratórios.

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Comparison of four antibiotics for inactivating leptospires in bull semen diluted in egg yolk extender and experimentally inoculated with Leptospira santarosai serovar guaricura

Cited by 7 publications

References 18 publications

Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil

Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil

Characterization of Leptospira interrogans serovar Pomona isolated from swine in Brazil

Uso De Estreptomicina Na Eliminação Da Leptospirúria Em Touros (Bos Taurus Indicus) Naturalmente Infectados Pelo Sorovar Hardjo

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