Comparison of fluorotyrosines and methionine uptake in F98 rat gliomas

Langen, Karl‐Josef; Jarosch, Michael; Mühlensiepen, H.; Hamacher, Kurt; Bröer, Stefan; Jansen, Paul; Zilles, Karl; Coenen, Heinz H.

doi:10.1016/s0969-8051(03)00023-4

Cited by 139 publications

(91 citation statements)

References 18 publications

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“…Experimental studies suggested that the transport mechanisms of 18 F-FET and 3 H-MET in glioma cells in vitro were comparable in principle (29). Studies in patients with peripheral tumors, however, found no uptake of 18 F-FET in most of the peripheral tumors, especially in lymphomas and most adenocarcinomas, which is in contrast to the results obtained with 11 C-MET and different tyrosine derivatives (30).…”

Section: Discussionmentioning

confidence: 95%

Comparison of O-(2-¹⁸F-Fluoroethyl)-l-Tyrosine and l-³H-Methionine Uptake in Cerebral Hematomas

Salber¹,

Stoffels²,

Oros-Peusquens³

et al. 2010

J Nucl Med

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Section: Discussionmentioning

confidence: 95%

Comparison of O-(2-¹⁸F-Fluoroethyl)-l-Tyrosine and l-³H-Methionine Uptake in Cerebral Hematomas

Salber¹,

Stoffels²,

Oros-Peusquens³

et al. 2010

J Nucl Med

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“…However, as discussed in previous studies (16,18,25), one can speculate that two factors play an important role in the uptake dynamics. First, expression of the L-amino acid transporter type 2 promotes bidirectional transport through the cell membrane (30,31), hence leading to higher and faster 18 F-FET uptake with increasing transporter density. Second, relative cerebral blood flow and tumor perfusion influence tracer delivery (and consequently the amount and speed of tumoral tracer uptake) and subsequent washout after intracellular accumulation of the unbound amino acid analag, which is not incorporated into proteins and may therefore not be trapped intracellularly (15).…”

Section: Discussionmentioning

confidence: 99%

Prognostic Significance of Dynamic ¹⁸F-FET PET in Newly Diagnosed Astrocytic High-Grade Glioma

et al. 2014

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Despite advances in diagnosis and the use of different therapeutic regimens in astrocytic high-grade glioma (HGG), the prognosis for patients remains grim. Additional pretherapeutic information is needed to tailor management. To gain additional prognostic information at primary diagnosis, we investigated the value ofWe retrospectively evaluated 121 patients who had a primary diagnosis of astrocytic HGG (51 World Health Organization [WHO] grade III; 70 WHO IV) and underwent dynamic 18 F-FET PET before histopathologic assessment. We assessed static parameters (maximal and mean tumoral standardized uptake value corrected for mean background activity in the contralateral hemisphere [SUV max / BG and SUV mean /BG, respectively], biologic tumor volume) and dynamic time-activity curves, including minimal time to peak (TTP min ). The prognostic influence of PET parameters and other clinical parameters on progression-free and overall survival was evaluated using uni-and multivariate Cox regression and Kaplan-Meier survival estimates. Results: In the group overall, median progression-free survival and overall survival were 12.2 and 21.9 mo. SUV max /BG, SUV mean /BG, and biologic tumor volume were significantly higher in WHO IV than in WHO III gliomas; median TTP min was 12.5 min in both groups. On univariate analysis, the factors age, WHO grade, O6-methylguanine-DNA methyltransferase promoter methylation status, contrast enhancement, initial treatment, and TTP min showed prognostic significance, with WHO grade, O6-methylguanine-DNA methyltransferase status, age, and TTP min remaining significant in the multivariate analysis. WHO grade and TTP min reached a similar fit for the prognostic evaluation. The prognosis of WHO III astrocytoma with an early TTP min of 12.5 min or less did not differ significantly from that of glioblastoma. Conclusion: Early TTP min is associated with worse outcome in patients with newly diagnosed astrocytic HGG. In the preoperative setting, TTP min can be a valuable noninvasive prognostic marker with comparable significance to WHO grade. Additionally, TTP min can help identify highly aggressive WHO III astrocytoma tumors and may help in adjusting standard treatment toward an individualized, risk-adapted therapy regime.

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“…LAT1 is supposed to be the only transporter that plays an important role in cell proliferation and shows increased transport activity in many cancer cells (15,18,30). However, there have been only a few in vitro studies using fluorine-labeled amino acids on tumor cell lines (7,31,32). Moreover, in our study we analyzed, for what is to our knowledge the first time, fluorine-labeled amino acid uptake in a comparison of tumor cells with primary human aortic endothelial cells and macrophages, which represent model cells for tumorassociated endothelial cells and macrophages, respectively.…”

Section: Discussionmentioning

confidence: 99%

L-Type Amino Acid Transporters LAT1 and LAT4 in Cancer: Uptake of 3-O-Methyl-6- 18F-Fluoro-L-Dopa in Human Adenocarcinoma and Squamous Cell Carcinoma In Vitro and In Vivo

Haase¹,

Bergmann²,

Fuechtner³

et al. 2007

Journal of Nuclear Medicine

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Expression of system L amino acid transporters (LAT) is strongly increased in many types of tumor cells. The purpose of this study was to demonstrate that 18 F-labeled amino acids, for example, 3-O-methyl-6-18 F-fluoro-L-dopa ( 18 F-OMFD), that accumulate in tumors via LAT represent an important class of imaging agents for visualization of tumors in vivo by PET. Methods: 18 F-OMFD uptake kinetics, transport inhibition, and system L messenger RNA expression were studied in vitro in human adenocarcinoma (HT-29), squamous cell carcinoma (FaDu), macrophages (THP-1), and primary aortic endothelial cells (HAEC) and in vivo in the corresponding mouse tumor xenograft models. Results: Uptake of 18 F-OMFD in all cell lines tested was mediated mainly by the sodium-independent high-capacity LAT. We found higher uptake in FaDu cells (V max , 10.6 6 1.1 nmol/min · mg of cell protein) and in the corresponding FaDu tumor xenografts than in the other cells and corresponding xenograft models studied. Quantitative messenger RNA analysis revealed that tumor cells and xenografts have a higher expression of LAT1 than do HAEC and THP-1 macrophages. However, only in the FaDu tumor model did an increased 18 F-OMFD uptake seem to be explained by increased LAT expression. Furthermore, we demonstrated a high expression of LAT4, a recently identified LAT. Conclusion: Our findings support the hypothesis that 18 F-OMFD is a tracer for visualization of tumor cells. 18 F-OMFD particularly seems to be a suitable tracer for diagnostic imaging of amino acid transport in poorly differentiated squamous cell head and neck carcinoma with increased LAT1 and LAT4 expression.

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Comparison of fluorotyrosines and methionine uptake in F98 rat gliomas

Cited by 139 publications

References 18 publications

Comparison of O-(2-¹⁸F-Fluoroethyl)-l-Tyrosine and l-³H-Methionine Uptake in Cerebral Hematomas

Comparison of O-(2-¹⁸F-Fluoroethyl)-l-Tyrosine and l-³H-Methionine Uptake in Cerebral Hematomas

Prognostic Significance of Dynamic ¹⁸F-FET PET in Newly Diagnosed Astrocytic High-Grade Glioma

L-Type Amino Acid Transporters LAT1 and LAT4 in Cancer: Uptake of 3-O-Methyl-6- 18F-Fluoro-L-Dopa in Human Adenocarcinoma and Squamous Cell Carcinoma In Vitro and In Vivo

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Comparison of fluorotyrosines and methionine uptake in F98 rat gliomas

Cited by 139 publications

References 18 publications

Comparison of O-(2-18F-Fluoroethyl)-l-Tyrosine and l-3H-Methionine Uptake in Cerebral Hematomas

Comparison of O-(2-18F-Fluoroethyl)-l-Tyrosine and l-3H-Methionine Uptake in Cerebral Hematomas

Prognostic Significance of Dynamic 18F-FET PET in Newly Diagnosed Astrocytic High-Grade Glioma

L-Type Amino Acid Transporters LAT1 and LAT4 in Cancer: Uptake of 3-O-Methyl-6- 18F-Fluoro-L-Dopa in Human Adenocarcinoma and Squamous Cell Carcinoma In Vitro and In Vivo

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Comparison of O-(2-¹⁸F-Fluoroethyl)-l-Tyrosine and l-³H-Methionine Uptake in Cerebral Hematomas

Comparison of O-(2-¹⁸F-Fluoroethyl)-l-Tyrosine and l-³H-Methionine Uptake in Cerebral Hematomas

Prognostic Significance of Dynamic ¹⁸F-FET PET in Newly Diagnosed Astrocytic High-Grade Glioma