Comparison of flow cytometric assays with isotopic assays of 51chromium‐labeled cells for estimation of red cell clearance or survival in vivo

Kumpel, Belinda M.; Austin, E. B.; Lee, D.; Jackson, Dave; Judson, P. A.; Chapman, G. E.

doi:10.1046/j.1537-2995.2000.40020228.x

Cited by 19 publications

(19 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6 However, current cell labels do not measure the life span of an age cohort, but rather, the survival of a randomly labeled RBC sample that contains cells of all ages. For many years 51 Cr, which binds tightly but noncovalently to hemoglobin, [17][18][19] has been the standard label for RBC life span determinations. More recently, biotin has been introduced as a nonisotopic label that is covalently attached to red cell membrane proteins and detected by flow cytometry.…”

Section: Introductionmentioning

confidence: 99%

Red cell life span heterogeneity in hematologically normal people is sufficient to alter HbA1c

Cohen

Franco²,

Khera

et al. 2008

Blood

385

370

View full text Add to dashboard Cite

Although red blood cell (RBC) life span is a known determinant of percentage hemoglobin A1c (HbA1c), its variation has been considered insufficient to affect clinical decisions in hematologically normal persons. However, an unexplained discordance between HbA1c and other measures of glycemic control can be observed that could be, in part, the result of differences in RBC life span. To explore the hypothesis that variation in RBC life span could alter measured HbA1c sufficiently to explain some of this discordance, we determined RBC life span using a biotin label in 6 people with diabetes and 6 nondiabetic controls.

show abstract

Section: Introductionmentioning

confidence: 99%

Red cell life span heterogeneity in hematologically normal people is sufficient to alter HbA1c

Cohen

Franco²,

Khera

et al. 2008

Blood

385

370

View full text Add to dashboard Cite

show abstract

“…Also, the RBC volume was determined by flow cytometry by exploiting antigen differences between transfused donor RBCs and the recipient's RBCs [89]. Finally, the survival of erythrocytes after transfusion or in patients with autoimmune hemolytic anemia was investigated by labeling RBCs with fluorochromes and subsequent quantification by flow cytometry [90,91].…”

Section: Immunohematologic Diagnostics Rbc Integrity and Immunologymentioning

confidence: 99%

Flow Cytometry in Transfusion Medicine: Development, Strategies and Applications

Greve¹,

Valet²,

Humpe³

et al. 2004

Transfus Med Hemother

View full text Add to dashboard Cite

Nowadays, flow cytometry represents an essential tool for the daily work in laboratories for transfusion medicine. After cytophotometry of immobilized cells, flow cytometry was developed since the 1960s using moving fluorescence-stained cells. This technique enabled the determination of several thousand cells per second, e.g. from blood, bone marrow, or organ-associated cell suspensions. The introduction of monoclonal fluorescence-labeled antibodies in diagnostics has further accelerated the development of flow cytometry. In the meantime, numerous cellular and nuclear properties can simultaneously be measured on single cell level in automated devices at high speed and precision. Internal standards and quality control systems further increased the accuracy of flow cytometric analyses. Flow cytometry is therefore likewise suitable for routine diagnostics in patients, quality control of blood components and scientific purposes in transfusion medicine. Nevertheless, many flow cytometric applications are related to counting of rare events or detection of cells with low antigen expression. Therefore, several pre-analytic and analytic factors (e.g. erythrocyte lysing procedures, cell gating strategies, etc.) may essentially affect the accurate determination of cell numbers and functions. In this issue, TRANSFUSION MEDICINE AND HEMOTHERAPY starts a recurrent sequence of reviews focused on ‘Flow Cytometry in Transfusion Medicine’. In order to introduce the flow cytometry series, this review summarizes the development, principles, and strategies of flow cytometry as an increasing diagnostic tool in medical laboratories. Also, current and possibly future flow cytometric applications in transfusion medicine are epitomized, e.g. quality control of blood products, immunohematologic diagnostics, hematopoietic progenitor cell transplantation, adoptive immunotherapy, and further therapeutic applications. In following issues of this journal, reviews of notable authors will then focus on special applications and give more detailed insights into single diagnostic fields.

show abstract

“…Early studies found FC to be superior to a differential agglutination method [98] and comparable to a 51 Cr survival study [99]. [106], and anti-D [107]. Most of these studies used FC to follow the survival of one or more whole units of blood.…”

Section: Detection and Quantitation Of Red Blood Cell Antigensmentioning

confidence: 99%

Flow Cytofluorometric Analysis in Red Blood Cell Immunology

Arndt¹,

Garratty²

2004

Transfus Med Hemother

View full text Add to dashboard Cite

Flow cytofluorometry (FC) is primarily used in the clinical laboratory for the analysis of white blood cells but also has applications in the study of red blood cell (RBC) immunology. When studying RBCs by FC it is important to avoid agglutination of the RBCs, which ironically is the endpoint for most immunohematologic assays. When studying low levels of RBC-bound antibody or RBC antigens with low copy number, background noise due to autofluorescence and nonspecific binding need to be taken into account. It is also important to ensure that reagents (e.g. antisera) used by FC have been standardized for use by FC. Applications of FC in RBC immunology include i) detection and quantitation of globulin (RBC-bound or free in serum), ii) detection and quantitation of RBC antigens, and iii) detection and quantitation of mixed cell populations. FC can be used to differentiate the amount of IgG on strongly IgG-coated RBCs, as well as to detect small amounts of RBC-bound IgG. FC has been used to quantitate antibody that is free in serum, e.g. anti-D in a pregnant woman or in immune globulin preparations. Many RBC antigens have been studied by FC to determine the number of antigen sites, the density distribution, and phenotype-related differences (e.g. zygosity). FC has been found to be very useful for studying mixed cell populations; applications include fetomaternal hemorrhage quantitation, determination of survival/ clearance of transfused RBCs, phenotyping of transfused patients’ RBCs, evaluation of bone marrow transplant engraftment, and study of RBC chimerism and mosaicism.

show abstract

Comparison of flow cytometric assays with isotopic assays of 51chromium‐labeled cells for estimation of red cell clearance or survival in vivo

Cited by 19 publications

References 27 publications

Red cell life span heterogeneity in hematologically normal people is sufficient to alter HbA1c

Red cell life span heterogeneity in hematologically normal people is sufficient to alter HbA1c

Flow Cytometry in Transfusion Medicine: Development, Strategies and Applications

Flow Cytofluorometric Analysis in Red Blood Cell Immunology

Contact Info

Product

Resources

About