In dairy cattle, bedding and its management contribute predominantly to the comfort of the cow, udder health and milk quality. The aim of the study is to assess the effect of different bedding materials such as concrete floor, rubber mats, coir pith and dried solid manure on microbial counts in milk and bedding materials in crossbred dairy cows. Twentyfour crossbred cows with six animals in each group at cattle farm was selected for the study for one lactation period spread over three different seasons. The control group (T 1 ) was maintained in concrete floor without any bedding materials. In (T 2 ) rubber mats of 1.2 m × 1.8 m × 0.025m area were provided on concrete floor. In (T 3 ) coir pith and (T 4 ) Dried solid manure (DSM) on concrete floor was the bedding. The aerobic plate count (APC), coliform count (CC) and total yeast and mould count (TYMC)were assessed in bedding materials to determine the microbial counts.Concrete