Comparison of Five Repetitive-Sequence-Based PCR Typing Methods for Molecular Discrimination of
            <i>Salmonella</i>
            <i>enterica</i>
            Isolates

Rasschaert, Geertrui; Houf, Kurt; Imberechts, Hein; Grijspeerdt, K.; Zutter, Lieven De; Heyndrickx, Marc

doi:10.1128/jcm.43.8.3615-3623.2005

Cited by 102 publications

(68 citation statements)

References 14 publications

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“…However, these authors did not specify which Salmonella serovars were tested, and probably they analyzed serovars with more genetic variance as compared to Enteritidis. A high discriminatory power also was found by Rasschaert et al (2005), since divided 13 S. Enteritidis into six clusters using the same ERIC primer set used in the present study. However, the differences found in the fingerprints of these strains consisted in bands of different intensity at 2000 bp, a size that was not analyzed in the present study, because bands larger than about 1500 bp displayed low resolution power in our experiments, what can explain the differences from our results.…”

Section: Discussionsupporting

confidence: 73%

Phenotypic and genotypic characterization of Salmonella Enteritidis isolates

Oliveira¹,

Bessa²,

Santos³

et al. 2007

Braz. J. Microbiol.

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In order to study the epidemiology of Salmonella Enteritidis outbreaks and determine the source of contamination so that a recurrence can be avoided, detailed characterization is necessary. Thus, the purpose of this study was to verify whether rep-PCR was able to discriminate among Salmonella Enteritidis isolates. Phage typing, detection of virulence genes and antimicrobial resistance testing were also associated to rep-PCR results. One hundred and two S. Enteritidis isolates from broiler carcasses, food, human, pigs, poultryrelated samples, and nine isolates from other countries were genotypically typed by REP-PCR, ERIC-PCR and BOX-PCR, collectively called rep-PCR. Phage typing, detection of virulence genes and antimicrobial resistance testing were also performed. Only three fingerprinting profiles were obtained with each rep-PCR method, with the majority of isolates belonging to the same profile. No relationship was observed between genotypic profile and year, place of isolation or source of infection. However, the less frequent rep-PCR profiles showed single antimicrobial resistance patterns. Although few strains isolated from swine were analyzed, different antimicrobial resistance patterns were observed. Furthermore, phage type 4 was not found in swine isolates. rep-PCR showed a lower discriminatory power as compared with antimicrobial resistance and phage typing, but the combination of genotypic and phenotypic methods was more discriminatory than any method alone, resulting in 48 different types.

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Section: Discussionsupporting

confidence: 73%

Phenotypic and genotypic characterization of Salmonella Enteritidis isolates

Oliveira¹,

Bessa²,

Santos³

et al. 2007

Braz. J. Microbiol.

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“…The similarities and differences in the ERIC-PCR banding patterns were analyzed by using Quantity-One software, version 4.5.2 (Bio-Rad Laboratories, Hercules, CA). The banding patterns were compared only for isolates that were processed in the same PCR run (26,33). Three stages of comparison were monitored.…”

Section: Methodsmentioning

confidence: 99%

Differences in the Fecal Concentrations and Genetic Diversities of Campylobacter jejuni Populations among Individual Cows in Two Dairy Herds

Rapp

Ross

Pleydell

et al. 2012

Appl Environ Microbiol

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cDairy cows have been identified as common carriers of Campylobacter jejuni, which causes many of the human gastroenteritis cases reported worldwide. To design on-farm management practices that control the human infection sourced from dairy cows, the first step is to acquire an understanding of the excretion patterns of the cow reservoir. We monitored the same 35 cows from two dairy farms for C. jejuni excretion fortnightly for up to 12 months. The objective was to examine the concentration of C. jejuni and assess the genetic relationship of the C. jejuni populations excreted by individual cows. Significant differences (P < 0.01) in C. jejuni fecal concentration were observed among the 35 cows, with median concentrations that varied by up to 3.6 log 10 · g ؊1 feces. A total of 36 different genotypes were identified from the 514 positive samples by using enterobacterial repetitive intergenic consensus (ERIC)-PCR. Although 22 of these genotypes were excreted by more than one cow, the analysis of frequencies and distribution of the genotypes by model-based statistics revealed a high degree of individuality in the C. jejuni population in each cow. The observed variation in the frequency of excretion of a genotype among cows and the analysis by multilocus sequence typing (MLST) of these genotypes suggest that excretion of C. jejuni in high numbers is due to a successful adaptation of a particular genotype to a particular cow's gut environment, but that animal-related factors render some individual cows resistant to colonization by particular genotypes. The reasons for differences in C. jejuni colonization of animals warrant further investigation.

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“…DNA fingerprints were compared by visual inspection. RAPD patterns were regarded as different if there were different bands on visual inspection (10,12,21).…”

Section: Bacterial Isolatesmentioning

confidence: 99%

Prevalence of Plasmid-Mediated AmpC β-Lactamases in a Chinese University Hospital from 2003 to 2005: First Report of CMY-2-Type AmpC β-Lactamase Resistance in China

et al. 2008

J Clin Microbiol

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The aim of this study was to investigate the prevalences of plasmid-mediated AmpC ␤-lactamases (PABLs) in isolates of Escherichia coli and Klebsiella spp. from a university hospital in China. A total of 1,935 consecutive nonrepeat clinical isolates of Escherichia coli, Klebsiella pneumoniae, and Klebsiella oxytoca were collected between January 2003 and July 2005. The isolates with cefoxitin zone diameters less than 18 mm (screen positive) were selected for PCR of the bla AmpC genes and sequencing. Fifty-four (2.79%) isolates harbored PABLs, as demonstrated by PCR and isoelectric focusing. Sequence analysis revealed the presence of bla DHA-1 and bla CMY-2 genes. The Southern blot hybridization studies confirmed that bla CMY-2 and bla DHA-1 were located on plasmids. Based on species, PABLs were detected in 4.29% (29 isolates of DHA-1 and 1 isolate of CMY-2) of K. pneumoniae, 1.91% (11 isolates of DHA-1 and 12 isolates of CMY-2) of E. coli, and 3.03% (1 isolate of DHA-1) of K. oxytoca isolates. In contrast to our anticipation, the occurrence rate of DHA-1-producing K. pneumonia significantly decreased (P < 0.01), from 7.54% in 2003 to 2.72% in 2004. The results of random amplified polymorphic DNA analysis indicate that the prevalences of DHA-1-producing K. pneumoniae and CMY-2-producing E. coli strains were not due to epidemic strains. In conclusion, DHA-1 was the most prevalent acquired AmpC beta-lactamase in this collection of isolates from a medical center in China, and DHA-1-producing K. pneumoniae was the most prevalent bacterium harboring a PABL. To the best of our knowledge, this is the first report of CMY-2-type AmpC ␤-lactamases in the Chinese mainland.Plasmid-mediated AmpC ␤-lactamases (PABLs) are derived from chromosomal ampC genes of the family Enterobacteriaceae, such as those of Citrobacter freundii, Enterobacter cloacae, and Aeromonas species.PABLs have been reported to occur in the United States, Korea, Japan, etc. (4,14,24), but there are seldom data indicating PABLs in the Chinese mainland (28,29). The aims of this study were to establish the prevalence rate of this resistance mechanism in isolates of Klebsiella pneumoniae, Klebsiella oxytoca, and Escherichia coli from a university hospital in Shanghai, China. The first identification of the CMY-2 AmpC ␤-lactamase in the Chinese mainland is also described. Antimicrobial susceptibility testing and screening of ␤-lactamases. Isolates were tested for susceptibility by the standard disk diffusion method, and the results were interpreted according to the guidelines of the CLSI (formerly NCCLS). Isolates with cefoxitin zone diameters less than 18 mm (4) were considered positive for the AmpC ␤-lactamase screening test and were selected for MIC, isoelectric focusing (IEF), PCR, and sequencing analyses. The MICs and the presence of extended-spectrum ␤-lactamases (ESBLs) were determined by using the ESBL confirmation panel (Dade-Behring, Sacramento, CA). The presence of ESBLs was also investigated by the CLSI-recommended disk screening and confirmatory tes...

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Comparison of Five Repetitive-Sequence-Based PCR Typing Methods for Molecular Discrimination of Salmonella enterica Isolates

Cited by 102 publications

References 14 publications

Phenotypic and genotypic characterization of Salmonella Enteritidis isolates

Phenotypic and genotypic characterization of Salmonella Enteritidis isolates

Differences in the Fecal Concentrations and Genetic Diversities of Campylobacter jejuni Populations among Individual Cows in Two Dairy Herds

Prevalence of Plasmid-Mediated AmpC β-Lactamases in a Chinese University Hospital from 2003 to 2005: First Report of CMY-2-Type AmpC β-Lactamase Resistance in China

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