Comparison of Five Protocols of Estrous Synchronization on Reproductive Performance of Hu Sheep

Yu, Xiaojie; Bai, Y. Y.; Yang, Jing; Zhao, Xiaokun; Zhang, Lei; Wang, Jing

doi:10.3389/fvets.2022.843514

Cited by 9 publications

(7 citation statements)

References 46 publications

(48 reference statements)

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“…Biehl et al showed that estrous behavior in sheep appeared mainly within 48 h after withdrawal of the progestogen sponge (Xiaojie Yu et al, 2022), which is also found in this study. The estrus induction rate in the case of sheep from the Țurcana breed is the highest in the case of the first batch between 24 -48 hours after the extraction of the sponges, followed by the interval of 12 -24 hours.…”

Section: Discussionsupporting

confidence: 85%

Synchronization of ovulation (FTET) in Turcana sheep as embryo recipients

Ciornei,

Șchiopu,

Pruteanu

et al. 2023

SPJVS

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Due to the fact that the farm wants to crossbreed sheep with meat breeds, it was opted for the fastest solution to bring genetic progress, namely embryo transfer, using sheep from the Turcan breed as embryo recipients and those from the Suffolk breed as embryo donors, so that finally the batch of receivers after parturition will produce a production of Suffolk lambs. Following the selection of recipients considering the standard criteria that include: general health, functional integrity of the genital tract and cyclic activity of the ovaries, two groups were formed for the induction and synchronization of estrus. The first batch consisted of 20 sheep and the second batch of 20. The first batch was subjected to a P4-PG-PMSG protocol and the second batch to the P4-PG-GnRH protocol. The two protocols consisted of the insertion of intravaginal sponges with flugestone acetate in a concentration of 20mg, to induce the progesterone phase, for 13 days, day 0 of the protocols being represented by the day when the progesterone sponges were applied. In continuation of the protocol, Prostaglandinf2α was used on day 12 in a double dose, in the morning and in the evening at a distance of 12 hours in a dose of 0.6 ml/administration/animal. On day 13, the progesterone sponges were removed and PMSG (Folligon) was administered in the case of the first batch at a dose of 600 IU/animal, and in the second batch GnRH (Receptal) was administered at a dose of 12 μg/animal ( 3ml/animal) followed by a dose of 600 IU HCG (Chorulon) 24 hours after GnRH administration. Ovulation was accurately assessed at the time of embryo transfer through laparotomy, due to the highlighting of the ovaries and their macroscopic analysis in the operative field, after identifying the CL on the ovary, their appearance and the number of CL on the ovary. However, the assessment of the rate of entry into heat was assessed 24 hours after the end of the protocol by biostimulating the receptors with the help of detector rams, thus the ewes that entered in estrus at 12 – 24 hours, 24 – 48 of hours or over 48 hours after completing the protocol. The results obtained in the case of the first batch having the highest rate of entering in estrus between 24 - 48 hours being 40%, followed by a rate of 30% between 12 - 24 hours, the lowest rate being 20% that entered in estrus after 48 hours, the second batch with GnRH had the highest rate of entry into estrus of 60% in the first 12-24 hours and the rate at 24-48 hours, respectively those that entered heat after 48 hours was 20%. Compared between the two groups, there were differences in the timing of ovulation assessment, as in the case of the first group with PMSG, a 60% ovulation rate was assessed with well-developed CL, 10% presented CL but these were unsuitable for embryo transfer, 10% presented ovarian cysts, and 10% did not ovulate, in the case of the second batch an ovulation rate of 60% was assessed and the rate of 40% represented the animals that did not ovulate, the difference between the two batches being the fact that it is observed in the case of the first batch rate of 10% with ovarian cysts, which indicates that the PMSG-based pharmaceutical is causing ovarian cysts. In conclusion, the therapeutic protocol used in the off-season for the induction of estrus and ovulation in Turcan sheep, in this study, resulted in the detection of estrus in 90% of the ewes subjected to the protocol, and the ovulation rate was 80% (60% with CL well developed and 20% were with poorly developed CL), regarding the first batch, and regarding the second batch 100% of ewes in oestrus were detected, but 60% of them ovulated with CL well developed. These results can be largely attributed to the reproduction seasonality of the sheep.

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Section: Discussionsupporting

confidence: 85%

Synchronization of ovulation (FTET) in Turcana sheep as embryo recipients

Ciornei,

Șchiopu,

Pruteanu

et al. 2023

SPJVS

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“…A more precise assessment was made in Jordan by Nasr et al [ 15 ], who reported a profit of USD 8.36 per ewe in flocks in which reproductive control had been applied. Specific financial benefits in reproductive control were also reported by Yu et al [ 16 ]. It should be noted that in Greece, prices for sheep and goat milk would be higher (by about 0.03–0.05 Euros) in late summer and early autumn, when animals subjected to reproductive control would be lambing/kidding, due to the limited quantities of milk available at the time.…”

Section: Discussionsupporting

confidence: 58%

Patterns of Reproductive Management in Sheep and Goat Farms in Greece

Lianou

Vasileiou

Michael

et al. 2022

Animals

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This paper reports findings regarding patterns of reproductive management in 325 sheep flocks and 119 goat herds throughout Greece. The objectives were to describe the patterns of reproductive management in sheep and goat farms, to highlight factors that were associated with these management patterns and the clinical application of the various reproductive management approaches in the farms and to establish potential associations with production outcomes. The median months of the start of the mating period for adult sheep and goats were May and June, respectively and for ewe lambs and replacement goats these were August and September, respectively. The median duration of the mating period was 2 months for adults and 1 month for replacements. The median ratio of female to male animals was 22:1 and 25:1 in sheep and goat farms, respectively. Control of reproduction was applied in 33.2% and 16.8% of sheep and goat farms, respectively, mostly by intravaginal progestagen sponges and less often by melatonin implants; multivariable analysis indicated five (availability of milking parlour, number of ewes in a farm, number of daily milkings, daily period spent by farmers at the farm, farming tradition in the family) and two (number of daily milkings, availability of milking parlour) predictors in sheep and goat farms, respectively. Pregnancy diagnosis ultrasonographically was performed in 36.6% and 16.8% of sheep and goat farms, respectively; multivariable analysis indicated as significant three (management system applied in farms, age of farmer, farming tradition in the family) and two (management system applied in farms, availability of milking parlour) predictors in sheep and goat farms, respectively. The control of reproduction and pregnancy diagnosis were associated with a higher number of vaccinations during pregnancy: 2.6 and 2.7, respectively, versus 2.2 and 2.1 in farms where they were not performed. The average per farm number of lambs/kids born per female animal was 1.33 ± 0.01 and 1.30 ± 0.02 for sheep and goat farms, respectively; five (control of reproduction, location of the farm, presence of working staff, collaboration with veterinary practice, breed of ewes) and two (control of reproduction, breed of female goats) predictors were identified in sheep and goat farms, respectively, for high numbers of lambs/kids. Lambs/kids were taken away from their dams at the age of 50 and 65 days, respectively. The average culling age (females, males) was 5.9 and 4.4 years and 6.9 and 4.9 years for sheep and goats, respectively. Farmers sourced animals to be used as replacements for those removed from the farm, mostly from the animals in their own farms, considering criteria related to milk production. Finally, the application of the control of reproduction was associated with increased milk production and the number of newborns, whilst an inverse correlation between the culling age of animals and milk production was seen.

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“…In an attempt to control animals' reproduction season, the farmers today use the application of exogenous hormones either to extend the reproduction activity in an ‘off‐season’ period or to premature this season, so as to finally prolong the milk or meat production throughout the year. Although the hormonal treatments are considered practical, efficient and easier by the means of implementation (Yu et al, 2022), they may be unaffordable for the producer as they have to be repeated every year (Manvi, 2014). In addition, it has been noted that exogenous hormones affect human health and, at the same time, leave a strong environmental footprint (Dardente et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Association of MTNR1A and GDF9 gene allelles with the reproductive performance, response to oestrus induction treatments and prolificacy, in improved and non‐improved local indigenous sheep breeds

Antonopoulou,

Giantsis,

Symeon

et al. 2023

Reprod Domestic Animals

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Sheep farming plays a crucial role for Mediterranean countries, wherefrom a plethora of dairy products is produced. Yet, numerous indigenous sheep breeds in temperate latitudes such as the Mediterranean present a serious drawback that milk production is impaired by the seasonality of reproduction. Towards the efforts for reduction of this phenomenon, the purpose of this study was to evaluate and compare different oestrus synchronization treatments, as well as to associate two genes alleles', namely the MTNR1A and the GDF9, with the response to those treatments. Three indigenous breeds were investigated (Florina, Chios and Karagouniko sheep) and inside each breed three different oestrus synchronization treatments were applied (A: intravaginal sponges, B: GNRH use and C: male effect). In group A, Florina ewes expressed oestrus at 90% in July and fecundity was 85%. Karagouniko and Chios ewes exhibited an oestrus expression of 100% with fecundity rates at 95% and 99%, respectively. In group B, Florina ewes expressed oestrus at 60% with fecundity at 57%, Karagouniko ewes expressed oestrus at 65% with fecundity at 54%, whereas Chios breed animals expressed oestrus at 87% with fecundity rate at 85%. In group C, 68% of the Florina breed expressed oestrus 20–25 days post ram induction, whereas this proportion was 84% and 94%, for Karagouniko and Chios breed, respectively. For the molecular analysis, partial segments of the two genes were sequenced and analysed, whereas alleles were scored based on the detected SNPs. All frequencies of the four detected SNPs in MTNR1A gene were statistically and significantly different in ewes that expressed oestrus in comparison with ewes that did not express oestrus in Florina and Karagouniko breeds concerning all treatments. Two SNP's were detected in GDF9 gene, G1 and FecG, from which, only the FecG mutation exhibited statistically significant difference in twins and triplets than in singles in Florina and Karagouniko breeds.

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Comparison of Five Protocols of Estrous Synchronization on Reproductive Performance of Hu Sheep

Cited by 9 publications

References 46 publications

Synchronization of ovulation (FTET) in Turcana sheep as embryo recipients

Synchronization of ovulation (FTET) in Turcana sheep as embryo recipients

Patterns of Reproductive Management in Sheep and Goat Farms in Greece

Association of MTNR1A and GDF9 gene allelles with the reproductive performance, response to oestrus induction treatments and prolificacy, in improved and non‐improved local indigenous sheep breeds

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