Comparison of Five Different Filters for the Removal of Leukocytes from Red Cell Concentrates

Abstract

The leukocyte depletion capacity and performance of 5 filters designed for filtration of red cell concentrates (RCC) were compared by counting leukocytes, measuring red cell volumes and by histological examination of the filters after use. To eliminate interdonor differences, 5 buffy-coat-poor RCC were pooled (in each of 10 experiments) and subsequently split up into the original bags. The RCC were passed over the Cellselect filter, a column filled with cellulose acetate, and over flat-bed polyester filters: the Cellselect Optima, the Pall RC 50, the Leukostop and the Sepacell R-500. The filtration was shortest with the Pall RC 50 (p less than 0.001 compared to the other 4 filters). Leukocyte removal was most effective with the cellulose acetate filter (p less than 0.01 compared to the other 4 filters) followed by the Cellselect Optima polyester filter (p less than 0.02 compared to the remaining 3 filters). Residual leukocytes did not exceed 50 x 10(6) for any brand of filter. Red cell recovery was similar for all 5 filters with mean values from 86.1 to 89.2%. The leukocyte numbers, counted in Türk's solution or in propidium iodide, gave comparable values in hemocytometers applying light microscopy or fluorescent microscopy, respectively. Histological examination showed that lymphocytes were mainly removed by trapping, whereas granulocytes showed a variable pattern: adhesion in presence of platelets or trapping.

Effects of Leucocyte Depletion on Rheologic Properties of Human CPDA-1 Blood

“…During filtration, white blood cells and platelets are removed partly by trapping and partly because of activation and subsequent adhesion to the filter [25, 26]. Leucocytes have a much lower deformability than RBCs [27]and may, therefore, affect high shear viscosity to a greater extent than one would expect from their small number (1,000 times less than RBCs).…”

Leukocyte Depletion of Red Cell Concentrates by Filtration: Influence of Blood Product Temperature

“…After filtraAmong the various methods for removing leukocytes from red cell concentrates (RCC), filtration procedures are reported to give the best results [1]. Numerous com mercial filters have now been investigated using simple and reliable techniques, although their effectiveness va ries considerably in the different published studies [2]. These discrepancies could be due to difficulties in count ing small numbers of leukocytes [3] and/or to the influence of the characteristics of the unit filtered, i.e.…”

Verzögerte intrakranielle Blutung bei einem Neugeborenen mit Alloimmunthrombozytopenie nach seriellen pränatalen Thrombozytentransfusionen

“…3) wurde jedoch aufgrund einer höheren Endkonzentration der Thrombozyten ein HPA1a-negatives Fremdspender-Thrombozytapheresekonzentrat transfundiert. Unmittelbar vor Transfusion wurde das Thrombozytenkonzentrat über einen Leukozytendepletionsfilter [8] in einer 10-ml-Spritze aufgezogen und unter sonographischer Kontrolle in die Nabelvene appliziert. Die Punktion erfolgte mit einer 22-Gauge-Spinalnadel (Durchmesser 0,7 mm) paraplazentar in die freie Nabelschnur [9].…”