Comparison of five automated hematology analyzers in a university hospital setting: Abbott Cell-Dyn Sapphire, Beckman Coulter DxH 800, Siemens Advia 2120i, Sysmex XE-5000, and Sysmex XN-2000

Abstract

To the best of our knowledge, this is the most comprehensive side-by-side comparison of five current top of the range routine hematology analyzers. Variable analyzer quality and parameter specific limitations must be considered in defining laboratory algorithms in clinical practice.

Erythroblastaemia in natalizumab-treated patients with multiple sclerosis

“…In considering the difference from our results, the two studies are not comparable due to a different study design, timing of data collection and instrumental acquisition. We used a newer automated NRBCs enumeration analyzer, which has a higher performance and sensitivity than previous instruments (Briggs et al, 2012b;Tantanate and Klinbua, 2015;Bruegel et al, 2015). The use of the same fully automated system and a microscopic verification for all our samples according to the CLSI standard H20-A2 (Koepke et al, 2010), ICSH (Briggs et al, 2014) and ISLH (Barnes et al, 2005) strengthens our results.…”

Reference intervals for iron-related blood parameters: results from a population-based cohort study (LIFE Child)

“…They are comparable only to a limited degree due to different representations as absolute and relative values. The literature has also described fluctuations due to different analyzers on multiple occasions [38,42,43]. Sysmex GmbH has described decreasing trends in adulthood [37], which partially matches the findings of this study.…”

“…It is virtually impossible for the generated measured values to harmonize perfectly due to the technical particulars of the different analyzers (different manufacturers, but also different models from a single manufacturer) [37]. As has been demonstrated, especially in the case of hemoglobin, the differences between various analyzers are minor compared to other hematological parameters [38]. Furthermore, differences can also be attributed to different criteria of inclusion and exclusion, as well as to the different statistical methods employed in the various studies.…”

Validation of a DNA methylation reference panel for the estimation of nucleated cells types in cord blood

“…Briefly, this automated hematology analyzer uses scatter light and fluorescence labeling information to uniquely differentiate cell types 17 . This automated analyzer has been shown to perform well compared to other commercial methods or manual microscopy evaluations 18 . Data were reported for each sample as absolute counts of nucleated red blood cells (nRBCs), eosinophils, lymphocytes, monocytes, neutrophils, and basophils.…”