Comparison of fetal bovine serum and human platelet lysate in cultivation and differentiation of dental pulp stem cells into hepatic lineage cells

Vasanthan, Punitha; Gnanasegaran, Nareshwaran; Govindasamy, Vijayendran; Abdullah, Ahmad Najib; Jayaraman, Pukana; Ronald, Veronica Sainik; Musa, Sabri; Kasim, Noor Hayaty Abu

doi:10.1016/j.bej.2014.04.007

Cited by 15 publications

(10 citation statements)

References 40 publications

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“…Apart from that, the presence of damaged liver conditioned medium can also augment the differentiation ability of MSCs into hepatocyte-like cells. In addition, our group have also worked on differentiation of DPSCs toward hepatocyte-like cells using the protocol established by Ishkitiev et al [ 82 -84 ] and Okada et al [ 85 ], and we have also observed similar characteristics as previously described [ 86 ].…”

Section: Dscs and Directed Differentiation Toward Hepatocyte-like Cellssupporting

confidence: 85%

Dental Stem Cell Differentiation Toward Endodermal Cell Lineages: Approaches to Control Hepatocytes and Beta Cell Transformation

Gnanasegaran¹,

Govindasamy²,

Nathan³

et al. 2016

Dental Stem Cells

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Section: Dscs and Directed Differentiation Toward Hepatocyte-like Cellssupporting

confidence: 85%

Dental Stem Cell Differentiation Toward Endodermal Cell Lineages: Approaches to Control Hepatocytes and Beta Cell Transformation

Gnanasegaran¹,

Govindasamy²,

Nathan³

et al. 2016

Dental Stem Cells

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“…The cells probably have carried some antigenic properties that might have triggered such reactions. It is suggested to incorporate xeno‐free culture conditions to alleviate this misfortune in future . The immuno‐modulatory properties as seen in the in vitro model again seconded the paracrine effects mediated by DPSCs.…”

Section: Discussionmentioning

confidence: 99%

“…The cells probably have carried some antigenic properties that might have triggered such reactions. It is suggested to incorporate xeno-free culture conditions to alleviate this misfortune in future [37].…”

Section: Discussionmentioning

confidence: 99%

Effect of dental pulp stem cells in MPTP‐induced old‐aged mice model

Gnanasegaran

Govindasamy

Simon

et al. 2017

Eur J Clin Investigation

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“…Among the different types of supplement from human source, platelet lysate was considered to be the best alternative to FBS because of its superiority in maintaining growth potential, genetic stability, immunomodulatory properties, and differentiation potential 110 , 113 , 125 - 130 . However, to produce clinical grade MSCs platelets free of infectious agents are of vital importance to prevent any possibility of disease transmission.…”

Section: Approaches To Enhance Engraftment and Regenerative Benefits mentioning

confidence: 99%

Optimization of Pre-transplantation Conditions to Enhance the Efficacy of Mesenchymal Stem Cells

Haque¹,

Kasim²,

Rahman³

2015

Int. J. Biol. Sci.

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Mesenchymal stem cells (MSCs) are considered a potential tool for cell based regenerative therapy due to their immunomodulatory property, differentiation potentials, trophic activity as well as large donor pool. Poor engraftment and short term survival of transplanted MSCs are recognized as major limitations which were linked to early cellular ageing, loss of chemokine markers during ex vivo expansion, and hyper-immunogenicity to xeno-contaminated MSCs. These problems can be minimized by ex vivo expansion of MSCs in hypoxic culture condition using well defined or xeno-free media i.e., media supplemented with growth factors, human serum or platelet lysate. In addition to ex vivo expansion in hypoxic culture condition using well defined media, this review article describes the potentials of transient adaptation of expanded MSCs in autologous serum supplemented medium prior to transplantation for long term regenerative benefits. Such transient adaptation in autologous serum supplemented medium may help to increase chemokine receptor expression and tissue specific differentiation of ex vivo expanded MSCs, thus would provide long term regenerative benefits.

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Comparison of fetal bovine serum and human platelet lysate in cultivation and differentiation of dental pulp stem cells into hepatic lineage cells

Cited by 15 publications

References 40 publications

Dental Stem Cell Differentiation Toward Endodermal Cell Lineages: Approaches to Control Hepatocytes and Beta Cell Transformation

Dental Stem Cell Differentiation Toward Endodermal Cell Lineages: Approaches to Control Hepatocytes and Beta Cell Transformation

Effect of dental pulp stem cells in MPTP‐induced old‐aged mice model

Optimization of Pre-transplantation Conditions to Enhance the Efficacy of Mesenchymal Stem Cells

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