Comparison of Euro-Collins Solution, Low-Potassium Dextran Solution Containing Glucose, and ET-Kyoto Solution for Lung Preservation in an Extracorporeal Rat Lung Perfusion Model

Abstract

We have recently developed a modified ET-Kyoto solution by adding N-acetylcysteine, nitroglycerin, and dibutyryl cyclic adenosine monophosphate to the previously reported ET-Kyoto solution. The purpose of this study was to compare the new ET-Kyoto solution with the regular Euro-Collins solution and a low-potassium dextran solution containing glucose (Perfadex^®) in an isolated rat lung perfusion model. Rat lung blocks were preserved with ET-Kyoto solution, with Euro-Collins solution, or with Perfadex for 4 h. Arterial oxygen tension, shunt fraction, peak inspiratory pressure, mean pulmonary arterial pressure, and pulmonary vascular resistance were assessed up to 50 min after reperfusion. ET-Kyoto solution provided a significantly better lung function after reperfusion than Euro-Collins solution and Perfadex, while Perfadex was also superior to the Euro-Collins solution. We conclude that lung preservation with ET-Kyoto solution is significantly superior to Euro-Collins solution and to Perfadex in an isolated rat lung perfusion model.

Exogenous surfactant application in a rat lung ischemia reperfusion injury model: effects on edema formation and alveolar type II cells

“…Hemodynamic and respiratory data were recorded during reperfusion as described previously [ 28 , 29 ]. Perfusate oxygenation (ΔPO 2 ), defined as the difference between oxygen tension of the perfusate collected from the left atrium after oxygenation (PO 2ox ) and of the deoxygenated perfusate of the pre-load pool (PO 2deox ), was used to assess the capability of gas exchange.…”

Trehalose: Current Use and Future Applications

“…[191][192][193][194][195]198,199 Trehalose has also been successfully used to cryopreserve human pancreatic endocrine tissue 260 and skin free-flaps of rabbit ears. 160…”

Pancreatic Ductal Perfusion at Organ Procurement Enhances Islet Yield in Human Islet Isolation

“…12 , 13 The potassium concentration in ETKS was designed at lower level than UWS, but higher than extracellular fluid, since 40 mmol/L of potassium had benefit in keeping vascular resistance lower in preclinical lung transplantation model when compared to specially prepared ETKS with much lower potassium level (20 mmol/L) and Euro-Collins solution with higher potassium (115 mmol/L). 14 , 15 ETKS includes unique ingredients of trehalose and gluconate, which help stabilize the cell membrane and prevent cell swelling. 16 , 17 ETKS showed less inhibition of collagenase activity than UWS but had comparable benefits in islet isolation.…”