Comparison of esterification methods for the gas chromatographic determination of indoprofen in plasma and urine

The absolute configuration of pentoxifylline alcohol produced by the microbial reduction of pentoxifylline using Rhodotorula rubra (ATCC 20129) was determined by O.R.D. spectroscopy and p.m.r. studies on the R-(+)-alpha-methoxy-alpha-(trifluoromethyl) phenylacetate (MTPA) ester. The development of a reverse-phase h.p.l.c. method for resolving the diastereomeric R-(+)-MTPA esters of racemic pentoxifylline alcohol and assignment of the elution order, allowed for the determination of the stereochemical purity of pentoxifylline alcohol produced by 13 microbial cultures shown previously to reduce pentoxifylline. A marked preference for S-alcohol production was observed with five organisms exhibiting complete stereoselectivity towards S-alcohol generation, and two producing the racemic alcohol.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Comparison of esterification methods for the gas chromatographic determination of indoprofen in plasma and urine

Cited by 2 publications

References 13 publications

Glass capillary gas chromatography of the serum fatty acid fraction via automatic injection of lipid extracts

Glass capillary gas chromatography of the serum fatty acid fraction via automatic injection of lipid extracts

Microbial models of mammalian metabolism: stereospecificity of ketone reduction with pentoxifylline

Contact Info

Product

Resources

About