Comparison of Ephestia kuehniella eggs sterilization methods for Trichogramma rearing

St-Onge, Mylène; Cormier, Daniel; Todorova, Silvia; Lucas, Éric

doi:10.1016/j.biocontrol.2013.12.006

Cited by 16 publications

(28 citation statements)

References 18 publications

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“…Several previous studies have shown that T. chilonis had comparable adult emergence and female progeny rates between UV-irradiated and untreated eggs of H. armigera (Romeis et al, 1997), and T. ostriniae had similar adult emergence rates on treated eggs of E. kuehniella (St-Onge et al, 2014). Similar percentages of adult emergence and female progeny were found on fertilized, unfertilized, and UVF hosts for all three Trichogramma parasitoids, with an exception of the adult emergence of T. japonicum.…”

Section: Discussionsupporting

confidence: 53%

“…In addition, UV-irradiated sterilization has been reported as a feasible application for long-term egg storage (Goldstein et al, 1983;Romeis et al, 1997;Moreno et al, 2009;Ksentini et al, 2014;St-Onge et al, 2014). UV irradiation treated host eggs such as Helicoverpa armigera (H€ ubner), Lobesia botrana (Denis & Schifferm€ uller), Ephestia kuehniella Zeller, showed little adverse effects on Trichogramma development, and therefore is an effective tool in rearing Trichogramma parasitoids.…”

Section: Introductionmentioning

confidence: 99%

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Effect of fertilized, unfertilized, and UV‐irradiated hosts on parasitism and suitability for Trichogramma parasitoids

Yang

Ying

et al. 2016

Entomologia Exp Applicata

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Trichogramma spp. (Hymenoptera: Trichogrammatidae) parasitoids have been commonly used as biological control agents in insect pest management. Host quality is believed to influence parasitism, host preference, and suitability for parasitoids. To date, limited studies have compared the parasitism of Trichogramma parasitoids on fertilized, unfertilized, and sterilized host eggs. Hence, we studied the performance of three Trichogramma egg parasitoids, Trichogramma japonicum Ashmead, Trichogramma chilonis Ishii, and Trichogramma leucaniae Pang & Chen, on fertilized, unfertilized, and ultraviolet (UV)-irradiated fertilized (UVF) eggs of rice moth, Corcyra cephalonica (Stainton) (Lepidoptera: Pyralidae). In a no-choice test, T. japonicum and T. leucaniae parasitized significantly more fertilized or UVF than unfertilized hosts, and T. chilonis parasitized significantly more UVF than either fertilized or unfertilized hosts. In a choice test, all three Trichogramma parasitoids parasitized UVF hosts the most, and unfertilized hosts the least. There were similar percentages of adult emergence and female progeny among fertilized, unfertilized, and UVF hosts for all three Trichogramma parasitoids, except that T. japonicum had significantly lower adult emergence on fertilized hosts. We also found that all three Trichogramma parasitoids developed slower on unfertilized hosts. Regardless of host treatments, T. leucaniae had the longest developmental time and T. chilonis had the shortest. We conclude that Trichogramma parasitoids prefer parasitizing UVF eggs of C. cephalonica without negative effects on their emergence and sex allocation.

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Section: Discussionsupporting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Effect of fertilized, unfertilized, and UV‐irradiated hosts on parasitism and suitability for Trichogramma parasitoids

Yang

Ying

et al. 2016

Entomologia Exp Applicata

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“…This may be because competition among individuals would be stronger in T. ostriniae and T. japonicum than that in T. dendrolimi , and this strong competition may cause a higher mortality in larger host eggs (Table ). In addition to a long and strong ovipositor, this may partially contribute to the fact that the two Trichogramma species cannot be reared on large host eggs, such as Antheraea pernyi Guérin‐Méneville (Hassan, Liscsinszky, & Zhang, ; Li, Chen, & Liu, ), whereas T. dendrolimi could be produced not only on small eggs, such as C. cephalonica (Wang, He, Zhang, Lu, & Babendreier, ) or Ephestia kuehniella eggs (St‐Onge, Cormier, Todorova, & Lucas, ), but also on large host eggs, such as eggs of A. pernyi (Hassan et al., ; Li et al., ).…”

Section: Discussionmentioning

confidence: 99%

Suitability assessment of three Trichogramma species in the control of Mythimna separata (Lepidoptera: Noctuidae)

Huang

Hua

Zhang

et al. 2017

J Applied Entomology

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Mythimna separata (Walker) is a serious cosmopolitan pest. Trichogramma species have been used worldwide as biological control agents of lepidopteran pests. However, no data on the control efficiency of Trichogramma species on this pest have been reported. We evaluated the suitability of M. separata eggs for three Trichogramma species, Trichogramma dendrolimi Matsumura, T. ostriniae Pang et Chen and T. japonicum Ashmead, and compared the suitability with that of Corcyra cephalonica (Stainton) eggs. The parasitism rate, development time, emergence rate, female proportion, body size and egg load of female offspring indicated that the M. separata egg is a suitable host for the three Trichogramma species. On the other hand, the non‐emergence rate and the relationship between egg load and body size reflected that M. separata eggs are less suitable for the three Trichogramma species than C. cephalonica eggs. Egg resorption occurred in T. japonicum 4 days after emergence.

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“…For UV sterilization treatments, the eggs were put in an UV Germicidal Sterilizer Mini 209 (YCC Products Inc., Placentia, CA, USA) at 10 cm from an 8 W lamp producing light at a 254‐nm wavelength for 15 min (St‐Onge et al. ). For the vacuum packing, 5 g of eggs was placed in a 200‐ml jar and vacuum was done at a 500 mm/Hg pressure with a V2240 pump (Jarden Corporation, Boca Raton, FL, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Freezing at −15°C can be a suitable sterilization method of Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) eggs for Trichogramma parasitism (St‐Onge et al. ), but long‐term conservation of host eggs at freezing temperatures has not been found to be suitable for parasitoid rearing. Hu et al.…”

Section: Introductionmentioning

confidence: 99%

Conservation of Ephestia kuehniella eggs as hosts for Trichogramma ostriniae

St-Onge

Cormier

Todorova³

et al. 2015

J Applied Entomology

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Mass rearing of numerous biological control agents depends on large amounts of factitious hosts like Ephestia kuehniella eggs. Moreover, production of some parasitoids, such as Trichogramma, requires hosts of high quality. The objective of this study was to determine the optimal conservation method that allows E. kuehniella eggs to remain suitable for parasitism and development of Trichogramma ostriniae for the longest period of time. Fifteen sterilization-conservation treatments were compared: nine consisting of submersion of the eggs in liquid nitrogen as a conservation mode, two consisting of sterilization of the eggs with UV and four consisting of sterilization with freezing at À15°C. Liquid nitrogen submersion of E. kuehniella eggs did not allow the production of T. ostriniae. The sterilization by exposition to UV light followed by conservation using vacuum packing and refrigeration at 4°C provided the longest conservation of E. kuehniella eggs for T. ostriniae rearing. It was the only treatment (75.4 AE 4.62%) for which the parasitism rate remained over 70% after 2 weeks.

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Comparison of Ephestia kuehniella eggs sterilization methods for Trichogramma rearing

Cited by 16 publications

References 18 publications

Effect of fertilized, unfertilized, and UV‐irradiated hosts on parasitism and suitability for Trichogramma parasitoids

Effect of fertilized, unfertilized, and UV‐irradiated hosts on parasitism and suitability for Trichogramma parasitoids

Suitability assessment of three Trichogramma species in the control of Mythimna separata (Lepidoptera: Noctuidae)

Conservation of Ephestia kuehniella eggs as hosts for Trichogramma ostriniae

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