Comparison of enzyme‐linked immunosorbent assay with indirect immunofluorescence assay for the diagnosis of <i>Mycoplasma pneumoniae</i> infection

Gao, Chunhai; Ji, Baifeng; Han, Chao; Wang, Mao‐Shui

doi:10.1002/jcla.22677

Cited by 3 publications

(3 citation statements)

References 22 publications

(26 reference statements)

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“…However, the positive rate of MP in our study is different from that in other previous studies ( Gao LW et al., 2019 ; Jiang et al., 2020 ), which may be due to differences in MP detection methods and enrolled populations. Chemiluminescence was used to detect IgM antibodies of serum MP in our study, whereas atypical pathogen DNA was detected in nasopharyngeal samples by a polymerase chain reaction in some studies ( Del Valle-Mendoza et al., 2017 ; Jiang et al., 2020 ; Kumar et al., 2020 ), or MP antibody was detected by the passive agglutination method ( Gao CH et al., 2019 ; Gao LW et al., 2019 ). PCR-based techniques are now considered more reliable, however, this method also has limitations, such as low sensitivity and positive predictive values ( Nilsson et al., 2008 ; Chang et al 2014 ; Kurkela et al 2019 ).…”

Section: Discussionmentioning

confidence: 82%

Epidemiological Study on Mycoplasma pneumoniae and Chlamydia pneumoniae Infection of Hospitalized Children in a Single Center During the COVID-19 Pandemic

Cai

Shou²,

Ye³

2022

Front. Cell. Infect. Microbiol.

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BackgroundSince the outbreak of COVID-19, a series of preventive and control measures in China have been used to effectively curb the spread of COVID-19. This study aimed to analyze the epidemiological characteristics of Mycoplasma pneumoniae (MP) and Chlamydia pneumoniae (CP) in hospitalized children with acute respiratory tract infection during the COVID-19 pandemic.MethodsMP IgM antibody and CP IgM antibody were detected in all hospitalized children due to acute respiratory tract infection in the Children’s Hospital Affiliated to Zhejiang University from January 2019 to December 2020. These data were compared between 2019 and 2020 based on age and month.ResultsThe overall detection rate of MP and CP in 2020 was significantly lower than that in 2019 (MP: 21.5% vs 32.9%, P<0.001; CP: 0.3% vs 0.9%, P<0.001). This study found a 4-fold reduction in the number of children positive for MP and a 7.5-fold reduction in the number of children positive for CP from 2019 to 2020. The positive cases were concentrated in children aged >1 year old. In 2019, the positive rate of MP was detected more commonly in children 3 years of age or older than in younger children. In 2020, the higher positive rate of MP reached a peak in the 3- to 6-year age group (35.3%). CP was detected predominantly in children aged 6 years older in 2019 and 2020, with positive rates of 4.8% and 2.6%, respectively. Meanwhile, the positive rates of MP in 2019 were detected more commonly in July, August and September, with 47.2%, 46.7% and 46.3%, respectively. Nevertheless, the positive rates of MP from February to December 2020 apparently decreased compared to those in 2019. The positive rates of CP were evenly distributed throughout the year, with 0.5%-1.6% in 2019 and 0.0%-2.1% in 2020.ConclusionsA series of preventive and control measures for SARS-CoV-2 during the COVID-19 pandemic can not only contain the spread of SARS-CoV-2 but also sharply improve the infection of other atypical pathogens, including MP and CP.

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Section: Discussionmentioning

confidence: 82%

Epidemiological Study on Mycoplasma pneumoniae and Chlamydia pneumoniae Infection of Hospitalized Children in a Single Center During the COVID-19 Pandemic

Cai

Shou²,

Ye³

2022

Front. Cell. Infect. Microbiol.

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“…However, for atypical pathogens, due to difficulty in isolation and their slow growth, which make it difficult to analyze the results in a short period of time, sputum cultures are rarely used in clinical practice. 19,20 In this study, we used a variety of detection methods such as serology, PCR, and urine antigen testing for three atypical pathogens. We listed the sensitivity and specificity of the detection methods in Table 6 after consulting many studies.…”

Section: Discussionmentioning

confidence: 99%

“…8,14,18 Another the positive patients were IgM positive, and IgM antibodies are recognized as early antibodies when the patient were infected by atypical pathogens, so it can also reflect the recent infection of the patients. 8,[19][20][21] We listed the sensitivity and specificity of the detection methods in Table 6 by consulting many literatures.…”

Section: Discussionmentioning

confidence: 99%

Atypical Pathogen Distribution in Chinese Hospitalized AECOPD Patients: A Multicenter Cross-Sectional Study

Feng¹,

Xu²,

Kang³

et al. 2021

COPD

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The proportion of atypical pathogens in patient with AECOPD within mainland China is unknown. The objectives of this study were to determine the distribution of atypical pathogens among Chinese patients with AECOPD, to evaluate the clinical characteristics of different atypical pathogen infections, and to compare different detection methods for atypical pathogens. Patients and Methods: Specimens were collected from patients with AECOPD from March 2016 to November 2018 at eleven medical institutions in eight cities in China. Double serum, sputum, and urine samples were obtained from 145 patients. Serological and nucleic acid tests were used to assess for Mycoplasma pneumonia and Chlamydia pneumoniae; serological, urinary antigen, and nucleic acid tests were applied to detect Legionella pneumophila. The clinical characteristics of atypical pathogen-positive andnegative groups were also compared. Results: The overall positivity rate for Mycoplasma pneumoniae was 20.69% (30/145), with the highest rate being 20.00% (29/145) when determined by passive agglutination.The overall positive rates for Chlamydia pneumoniae and Legionella pneumophila were 29.66% (43/145) and 10.34% (15/145), respectively. The most common serotype of Legionella pneumophila was type 6. The maximum hospitalized body temperature, ratio of eosinophils, C-reactive protein (CRP) level, and procalcitonin (PCT) level of the Mycoplasma pneumoniae-positive group were significantly higher than those of the Mycoplasma pneumoniae-negative group. Patients in the Chlamydia pneumoniae-positive group smoked more, had higher proportions of comorbidities and frequent aggravations in the previous two years than those in the Chlamydia pneumoniae-negative group. Furthermore, the forced expiratory volume in one second to forced vital capacity (FEV 1 / FVC) ratio assessment of lung function was higher, and the concentration of arterial blood bicarbonate (HCO 3 − ) was lower in the Legionella pneumophila-positive group than in the Legionella pneumophila-negative group. Conclusion: Overall, atypical pathogens play an important role in AECOPD. Regarding the testing method, serological testing is a superior method to nucleic acid testing.

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A Study on the Distribution of Mycoplasma pneumoniae Infectious Disease in Children from 2016 to 2018 According to Age

Choi,

Kim

2023

Pediatr Infect Vaccine

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Comparison of enzyme‐linked immunosorbent assay with indirect immunofluorescence assay for the diagnosis of Mycoplasma pneumoniae infection

Cited by 3 publications

References 22 publications

Epidemiological Study on Mycoplasma pneumoniae and Chlamydia pneumoniae Infection of Hospitalized Children in a Single Center During the COVID-19 Pandemic

Epidemiological Study on Mycoplasma pneumoniae and Chlamydia pneumoniae Infection of Hospitalized Children in a Single Center During the COVID-19 Pandemic

Atypical Pathogen Distribution in Chinese Hospitalized AECOPD Patients: A Multicenter Cross-Sectional Study

A Study on the Distribution of Mycoplasma pneumoniae Infectious Disease in Children from 2016 to 2018 According to Age

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