Comparison of enzyme-linked immunosorbent assay, radioimmunoassay, complement fixation, anticomplement immunofluorescence and passive haemagglutination techniques for detecting cytomegalovirus IgG antibody.

Abstract

SUMMARY The radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) techniques were found to be comparable in sensitivity and specificity for detecting cytomegalovirus IgG antibody, and 10 to 100 times more sensitive than complement-fixation (CF), anticomplement immunofluorescence (ACIF) and passive haemagglutination (PHA). In screening tests for antibody, the frequency of false-positive and -negative results was 0 6% for RIA and ELISA, 1-5% for CF, 1 6% for ACIF and 3 6% for PHA. PHA was the least satisfactory test, largely because of technical problems.Cytomegalovirus (CMV) infection is an important cause of congenital brain damage' and is also a major complication of both prolonged immunosuppressive therapy, especially in patients with organ transplants,2 and multi-donor blood transfusions.3 For serological diagnosis of infection, as well as for screening for antibody in patients and in blood donors, the solid-phase indirect radioimmunoassay (RIA)" and enzyme-linked immunosorbent assay (ELISA)7-9 techniques offer distinct improvements in sensitivity over previous methods. Although the principle of both tests, based on the detection of antigen-antibody reactions by means of a labelled anti-antibody, is the same, each possesses its own particular technical advantages and disadvantages, and both require their own expensive equipment for the reading of the results. There is still a lack of data on how they compare in sensitivity and specificity. The present study was undertaken to compare the two methods for the detection of CMV IgG and to evaluate them against the older techniques of complement-fixation (CF), passive haemagglutination (PHA) and anticomplement immunofluorescence (ACIF).Material and methods virus-specific binding index (SBI) of >2 was taken to indicate the presence of virus-specific antibody and S E R A antibody titres were calculated graphically from the These were collected from women attending the ante-reciprocal of the serum dilution having an SBI of 2-0.

Review of cytomegalovirus seroprevalence and demographic characteristics associated with infection

“…Because of its superior performance, the vast majority of the studies used an enzyme-linked immunosorbent assay (ELISA) to detect CMV-specific IgG. When simply determining whether a person is antibody positive or negative, ELISA is only marginally more sensitive than CF or ACIF [19,20]. However, when measuring titres, the ELISA format is 10-100 times more sensitive than CF or ACIF [20].…”

SAĞLIKLI BİREYLERİN SERUMLARINDA CYTOMEGALOVIRUS (CMV) IgG ANTİKORLARININ ELISA İLE ARAŞTIRILMASI

“…CMV organizmada genellikle asemptomatik latent infeksiyon oluşturmakta ve antikor varlığına rağmen, immünosupresyon gibi çeşitli faktörlere bağlı olarak reaktivasyon gösterebilmektedir (8,16). Bu virüs infeksiyonu ve reaktivasyonu, kompleman birleşme deneyi (CF), immünofloresan antikor deneyi (İFA), indirekt hemaglutinasyon (IHA) gibi serolojik yön-temlerle belirlenebilmektedir (3,4). Enzyme-linked immunosorbent assay (ELISA) yöntemi de CMV enfeksiyonlarının tanımında yaygın olarak kullanıl-maktadır (9,12).…”

CMV and transfusions, an old story that's not quite over yet

“…84 Though false negative reactions are rare, and the sensitivity of ELISA for CMV is high, they do occur. [85][86][87] False negative serological reactions may be due to technical reasons, antigenic heterogeneity in CMV, 88 or because some individuals are poor antibody responders. It has been known that more than one-half of CMV-seronegative individuals have CMV DNA.…”