Comparison of enzyme activities in oxalate synthesis betweenspinacia oleraceal. andbrassica campestrisL.

Oxalate accumulation and regulation is independent of glycolate oxidase in rice leaves

“…5) and correlation analysis assured that oxalate content and GLO activities were not significantly correlated (data not shown). Previous reports have also indicated that GLO activity and kinetic property were not different between the oxalateaccumulating and oxalate-non-accumulating plant species (Watanabe et al, 1995;Li et al, 2000), and the K m of GLO for glyoxylate was determined to be much higher than the measured physiological glyoxylate concentration (Davies and Asker, 1983;Libert and Franceschi, 1987). Here, it was also shown that the K m of rice GLO for glyoxylate was ;4 mM while glyoxylate concentration in cells could be much lower than this value (Heupel and Heldt, 1994; Fig.…”

“…GLO has long been considered as a key component mediating oxalate biosynthesis in some plants (Millerd et al, 1963a, b;Piquemal et al, 1980;Chang and Huang, 1981;Franceschi, 1987;Li and Franceschi, 1990), yet its role in regulating oxalate accumulation is not defined. It has been observed that GLO activity and its kinetic property did not differ between oxalate-accumulating and oxalate non-accumulating plant species (Watanabe et al, 1995;Li et al, 2000) and that the K m of GLO for glyoxylate was determined to be much higher than the measured physiological glyoxylate concentration (Davies and Asker, 1983;Libert and Franceschi, 1987). In this study, the role of GLO in oxalate accumulation and regulation was investigated in rice plants.…”

Identification of long needle crystals and distribution of oxalate salts inNarcissuscv. ‘Garden Giant’

“…Oxalate salts accumulate in various species of higher plants, such as spinach (Goh and Vityakon 1989;Prokakis and Gonzalez 1990;Kameno et al 1991;Kleczkowski et al 1991;Fujii et al 1993;Takebe et al 1995a,b;Watanabe et al 1995), begonia (Sasaki 1963), Phaseolus vulgaris (Zindler-Frank et al 1989; Barnabas and Arnott 1991), plum and cherry (Sanchez-Alonso and Lachica 1988), and rice seedlings (Ivanov et al 1991). Oxalic acid has been considered to be a by-product of plant metabolism.…”

“…Furthermore, the rate of oxalate synthesis via glycolate depends on the age of the leaves (Fujii et al 1993). However, not all C 3 plants accumulate oxalate, and Watanabe et al (1995) suggested that the glycolate oxidizing enzyme in spinach, an oxalate accumulator, may differ from the enzyme oxidizing glyoxylate in the non-accumulator C 3 plant, Brassica campestris L.…”