Comparison of enterococcal populations in animals, humans, and the environment - a European study

Kühn, Inger; Iversen, Aina; Burman, Lars; Olsson‐Liljequist, Barbro; Franklin, A.; Finn, Maria; Aarestrup, Frank Møller; Seyfarth, Anne Mette; Blanch, Anicet R.; Vilanova, X; Taylor, Huw; Caplin, Jonathan; Moreno, Miguel A.; Domı́nguez, Lucas; Herrero, I.; Möllby, R.

doi:10.1016/s0168-1605(03)00176-4

Cited by 132 publications

(100 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Among these, the species-specific PCR identified 78 E. faecalis (23.6%) and 48 E. faecium (14.5%) strains, with the remaining 204 (61.8%) strains considered to belong to other Enterococcus species. A previous study covering several European Union countries showed that E. faecalis and E. faecium constituted 50 to 75% of isolated enterococci in pig feces samples (8). We are not able to explain why the two species constituted such a surprisingly low proportion of Enterococcus spp.…”

Section: Resultscontrasting

confidence: 72%

Impact of Medicated Feed on the Development of Antimicrobial Resistance in Bacteria at Integrated Pig-Fish Farms in Vietnam

Dang

Petersen

Truong

et al. 2011

Appl Environ Microbiol

View full text Add to dashboard Cite

Integrated livestock-fish aquaculture utilizes animal excreta, urine, and feed leftovers as pond fertilizers to enhance the growth of plankton and other microorganisms eaten by the fish. However, antimicrobial-resistant bacteria may be transferred and develop in the pond due to selective pressure from antimicrobials present in animal feed, urine, and feces. In an experimental pig-fish farm located in periurban Hanoi, Vietnam, nine piglets were provided feed containing 5 g of tetracycline (TET)/kg pig weight/day and 0.45 g of enrofloxacin (ENR)/kg pig weight/day during the second and fourth (last) months of the experiment. The aim of this study was to determine the association between the provision of pig feed with antimicrobials and the development of antimicrobial resistance, as measured in a total of 520 Escherichia coli and 634 Enterococcus strains isolated from pig manure and water-sediment pond samples. MIC values for nalidixic acid (NAL) and ENR showed that E. coli and Enterococcus spp. overall exhibited significant higher frequencies of resistance toward NAL and ENR during the 2 months when pigs were administered feed with antimicrobials, with frequencies reaching 60 to 80% in both water-sediment and manure samples. TET resistance for both indicators was high (>80%) throughout the study period, which indicates that TET-resistant E. coli and Enterococcus spp. were present in the piglets before the initiation of the experiment. PCR-based identification showed similar relative occurrences of Enterococcus faecium, Enterococcus faecalis, and other Enterococcus spp. in the water-sediment and manure samples, suggesting that Enterococcus spp. isolated in the ponds originated mainly from the pig manure. The development of antimicrobial resistance in integrated animal husbandry-fish farms and possible transfers and the impact of such resistance on food safety and human health should be further assessed.

show abstract

Section: Resultscontrasting

confidence: 72%

Impact of Medicated Feed on the Development of Antimicrobial Resistance in Bacteria at Integrated Pig-Fish Farms in Vietnam

Dang

Petersen

Truong

et al. 2011

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…This finding is in agreement with previous studies that reported the prevalence of these strains in fecal contaminated environmental samples [34][35][36] and showed a particular ability of E. faecium and E. fecalis to acquire and maintain resistance traits [37,38].…”

Section: E Flores Et Alsupporting

confidence: 93%

Presence of Multidrug-Resistant E. coli, Enterococcus spp. and Salmonella spp. in Lakes and Fountains of Porto, Portugal

Flores¹,

Loureiro²,

Bessa³

et al. 2013

JWARP

View full text Add to dashboard Cite

Urban lakes and fountains provide recreational activities that could facilitate the contact between humans, animals and biological agents. The objective of this work was to assess the water quality and safety of 17 lakes and 13 fountains in the city of Porto (Portugal), by detecting the presence of Escherichia coli, enterococci and Salmonella spp., and analyzing their antimicrobial resistance. The mean number of E. coli colony forming units (CFU) was higher, both in lakes (2.67 × 10 3 CFU/100 ml) and fountains (3.52 × 10 2 CFU/100 ml), in comparison to enterococci counts: 5.60 × 10 2 and 4.10 × 10 CFU/100 ml, respectively. By screening a set of 26 multidrug-resistant E. coli (including seven ESBL-producing isolates) for virulence genes, we found strains carrying the fimH, chuA, cvaC, cnf1, hlyA, TSPE4.C2 and yjaA genes. Phylogenetic analyses have shown that the majority of E. coli strains belong to groups A and B1. Salmonella spp. was found in two lake water samples. Generally, the fecal contamination was notorious and worrisome, requiring preventive measures to minimize the negative impacts on human health. Finding the contamination source is definitely important so that interventions can be more effective.

show abstract

“…The dominant species was E. faecalis, regardless of the source of the strains (Table 1). These results are consistent with data from other European countries (Kuhn et al 2003). …”

supporting

confidence: 93%

Undomesticated Animals as a Reservoir of Multidrug-ResistantEnterococcusin Eastern Poland

Nowakiewicz

Ziółkowska

Zięba

et al. 2014

Journal of Wildlife Diseases

View full text Add to dashboard Cite

ABSTRACT:To assess implications for public health we compared the resistance of Enterococcus spp. strains to antibacterial drugs in wild and exotic animals with strains originating in domesticated animals and characterized correlations between Enterococcus species, the source of the isolate, and the degree of resistance to selected antibiotics. All strains, regardless of source, were susceptible to blactams, gentamicin, linezolid, and teicoplanin; the highest resistance was to kanamycin, quinupristin, and rifampicin. Thirteen strains from undomesticated animals were resistant to vancomycin, and one strain, from a fox, was resistant to streptomycin (high-dose). Multidrug-resistant strains accounted for 46% of the strains from wild animals and 59% of the strains from an exotic animal (the Russian tortoise; Testudo horsfieldii). Despite the relatively low level of resistance in the strains isolated from wild and exotic animals, the large number of intermediately susceptible strains in these groups is an indication of the evolutionary character of the development of resistance, suggesting that these animals may be potential reservoirs of Enterococcus strains resistant to a wide panel of currently used antibiotics.

show abstract

Comparison of enterococcal populations in animals, humans, and the environment - a European study

Cited by 132 publications

References 19 publications

Impact of Medicated Feed on the Development of Antimicrobial Resistance in Bacteria at Integrated Pig-Fish Farms in Vietnam

Impact of Medicated Feed on the Development of Antimicrobial Resistance in Bacteria at Integrated Pig-Fish Farms in Vietnam

Presence of Multidrug-Resistant <i>E. coli</i>, <i>Enterococcus</i> spp. and <i>Salmonella</i> spp. in Lakes and Fountains of Porto, Portugal

Undomesticated Animals as a Reservoir of Multidrug-ResistantEnterococcusin Eastern Poland

Contact Info

Product

Resources

About

Comparison of enterococcal populations in animals, humans, and the environment - a European study

Cited by 132 publications

References 19 publications

Impact of Medicated Feed on the Development of Antimicrobial Resistance in Bacteria at Integrated Pig-Fish Farms in Vietnam

Impact of Medicated Feed on the Development of Antimicrobial Resistance in Bacteria at Integrated Pig-Fish Farms in Vietnam

Presence of Multidrug-Resistant &lt;i&gt;E. coli&lt;/i&gt;, &lt;i&gt;Enterococcus&lt;/i&gt; spp. and &lt;i&gt;Salmonella&lt;/i&gt; spp. in Lakes and Fountains of Porto, Portugal

Undomesticated Animals as a Reservoir of Multidrug-ResistantEnterococcusin Eastern Poland

Contact Info

Product

Resources

About

Presence of Multidrug-Resistant <i>E. coli</i>, <i>Enterococcus</i> spp. and <i>Salmonella</i> spp. in Lakes and Fountains of Porto, Portugal