Comparison of endpoints relevant to toxicity assessments in 3 generations of CD-1 mice fed irradiated natural and purified ingredient diets with varying soy protein and isoflavone contents

Camacho, Luísa; Lewis, Sherry M.; Vanlandingham, Michelle; Juliar, Beth E.; Olson, Greg R.; Patton, Ralph E.; Costa, Gonçalo Gamboa da; Woodling, Kellie A.; Sepehr, Estatira; Bryant, Matthew; Doerge, Daniel R.; Basavarajappa, Mallikarjuna S.; Felton, Robert P.; Delclos, K. Barry

doi:10.1016/j.fct.2016.05.014

Cited by 15 publications

(13 citation statements)

References 78 publications

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“…Mice food was in the form of pellets. Genistein supplemented diet as well as control diets were sterilized by irradiation (20-50kGY) as recommended by Teklad Envigo [ 36 ].…”

Section: Methodsmentioning

confidence: 99%

Impact of genistein on the gut microbiome of humanized mice and its role in breast tumor inhibition

Paul

Royston

et al. 2017

PLoS ONE

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Since dietary polyphenols can have beneficial effects in prevention and treatment of cancer, we tested the hypothesis that breast cancer patients’ intestinal microbiota is modulated by genistein (GE), an isoflavone found in soy, and that microbial alterations may offset the side effects brought about by chemotherapy. We demonstrated successful humanization of germ-free mice by transplanting fecal samples from breast cancer patients before and after chemotherapy. Mice were then grouped based on chemotherapy status and GE or control diet. We did not find any significant differences between pre-chemotherapy and post-chemotherapy bacterial composition and abundances. Germ-free mice on a GE diet showed differences in microbial composition as compared to mice on control diet. Four weeks after introduction of the customized GE diet, there was distinct clustering of GE-fed mice as compared to the control-fed group. In the gut microbiome of GE-treated humanized mice, there was an increase in abundance of genera Lactococcus and Eubacterium. Phylum Verrucomicrobia showed statistically significant (p = 0.02) differences in abundances between the GE-fed and control-fed groups. There was an increase in bacteria belonging to family Lachnospiraceae and Ruminococcaceae in GE-fed mice. Marked changes were observed in GE catabolism in mice humanized with fecal material from two of three patients’ post-chemotherapy with complete disappearance of 4-ethylphenol and 2-(4-hydroxyphenol) propionic acid conjugates. The post-tumor samples did not show any distinct clustering of the gut microbiota between the two diet groups. There was an increase in latency of about 25% for tumor growth of the humanized mice that were on a GE diet as compared to humanized mice on a control diet. The average tumor size for the GE group was significantly decreased compared to the non-GE group. Collectively, our results suggest that the intestinal microbiota becomes altered with a GE diet before induction of tumor. Our findings indicate that GE modulates the microbiome in humanized mice that may contribute to its effects on increasing the latency of breast tumor and reducing tumor growth.

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“…Mice food was in the form of pellets. Genistein supplemented diet as well as control diets were sterilized by irradiation (20-50kGY) as recommended by Teklad Envigo [ 36 ].…”

Section: Methodsmentioning

confidence: 99%

Impact of genistein on the gut microbiome of humanized mice and its role in breast tumor inhibition

Paul

Royston

et al. 2017

PLoS ONE

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“…As semi‐purified diets are generally favoured because they provide more uniformity and reproducibility, we used semi‐purified diet in our study. There are several studies reported in the literature using semi‐synthetic diets for various experimental purposes in various experimental animal models (Camacho et al, ; Chan, ; Del Bas et al, ; Horton, Cuthbert, & Spady, ; Kang, Shin, Park, & Lee, ; Kopeć & Leszczyńska, ; Krasnow, Nguyen, & Marks, ; Mawatari et al, ; Muga & Chao, ; Rousseaux, Schiefer, & Hancock, ; Wang, Jones, Pischel, & Fairow, ).…”

Section: Discussionmentioning

confidence: 99%

“…Cornstarch as a source of carbohydrate is commonly used for many of the studies using semi‐synthetic diets in rats and mice (Annapurna et al, ; Camacho et al, ; Del Bas et al, ; Kang et al, ; Kopeć & Leszczyńska, ; Krasnow et al, ; Mawatari et al, ; Rousseaux et al, ; Venu et al, ). In studies carried out in rats, although two widely used control diets, a semi‐purified diet (SD) or chow‐based diet (ND) produced very different outcomes in the lipid metabolism of lactating rats and their offspring, energy intakes, growth and fertility were not affected (Del Bas et al, ).…”

Section: Discussionmentioning

confidence: 99%

Semi‐synthetic diet versus diet using natural ingredients—Comparative study in female Golden Syrian hamsters

Mohammed

Hemalatha

Qadri

et al. 2019

Animal Physiology Nutrition

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| INTRODUC TI ONLaboratory animals invariably serve as the basis for all scientific discoveries and technological advancements in the medical field including almost all medical knowledge, treatment regimes and medical device development. Proper selection of animal model for research is the key factor for extrapolation of animal research findings to humans, thus helping to improve the lives of people and other animals (Rand, 2008). Of the several factors that influence reproducibility of results, diet is an important environmental factor besides microbiological and genetic factors that affect reproduction, growth, disease and responses of laboratory animals to scientific manipulation. AbstractSemi-synthetic diets (SSD) are recommended and are widely used to carry out experiments in rodents. However, in our experiments planned to carry out generation studies in female Golden Syrian hamsters using semi-synthetic diets, it was observed that the hamsters did not conceive as a result of decreased food intake. In this paper, we present the effects of both semi-synthetic diets and natural source diets (NSD) on food intake, body weight and reproductive performance of this species. Four-weekold female hamsters were equally divided into 3 groups and initially acclimatized for 2 weeks on natural chow diet (NCD). Thereafter, they were fed either control diet, high fat diet (HFD) or low protein diet (LPD) based on semi-synthetic/natural source ingredients until 12 weeks. Daily food intake and weekly body weights were monitored. Hamsters were kept for mating for about 2 weeks from 10th week onwards, during which the pregnancy confirmation test was done using standard vaginal smear examination. In all the groups fed SSD, the food intake was very poor, hamsters lost body weight and did not conceive, thus preventing us from carrying out further experiments. Hamsters fed NCD/NSD ingested more than twice as much as hamsters fed SSD (7-8 g/day/hamster against 3 g/day/hamster on average respectively).Based on the results of the current research, we conclude that the routinely used semi-synthetic diet is not suitable for carrying out studies in female hamsters. We suggest that scientists must also consider the unusual biological characteristics of a given species besides other biological factors. It is therefore critical to select appropriate biological models and diets that provide optimal sensitivity and specificity to accomplish the research objectives. K E Y W O R D Sbody weights, female hamsters, food intake, natural source diet, reproductive performance, semi-synthetic diet

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“…b Mean body weights at the later time points, weeks 36 to 52, were 10-13% higher than control means in the 2.5 μg BPA/kg bw/day. The Dunnett-corrected p-values were 0.080, 0.058, 0.071, 0.065, and 0.062, for weeks 36 75 ± 1 (50) 76 ± 1 (48) 78 ± 1 (46) 76 ± 1 (49) 74 ± 1 (50) 76 ± 1 (46) 76 ± 1 (26) 80 ± 2 * (26) 8 196 ± 3 (50) 201 ± 3 (48) 202 ± 2 (46) 199 ± 2 (49) 198 ± 3 (50) 204 ± 3 b (45) 200 ± 4 (26) 212 ± 4 * (26) 12 266 ± 4 (50) 269 ± 4 (48) 270 ± 4 (46) 267 ± 3 (49) 266 ± 4 (50) 269 ± 3 (45) 269 ± (26) 268 ± 6 (26) 16 302 ± (50) 305 ± 5 (47) 303 ± (46) 299 ± 3 (49) 300 ± (50) 302 ± 4 (45) 299 ± (26) 299 ± 7 (26) 20 324 ± 6 (50) 327 ± 6 (47) 325 ± 6 (46) 319 ± 4 (49) 319 ± 5 (50) 321 ± 4 (45) 320 ± 7 (26) 320 ± 9 (26) 24…”

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confidence: 94%

“…There were also no significant treatment effects in the sensitivity analysis that excluded all animals that overlapped with animals treated with 250,000 μg BPA/kg bw/day (see Materials and Methods, Statistical Methods). 474 ± 11 (50) 459 ± 11 (49) 477 ± 11 (46) 497 ± 13 (49) 506 ± 10 49482 ± 11 (47) 496 ± 12 (50) 480 ± 12 (49) 495 ± 12 (45) 516 ± 14 (48) 524 ± 11 (46) 502 ± 11 (47) 515 ± 12 (50) 496 ± 12 (48) 513 ± 14 (44) 536 ± 15 (48) 549 ± 13 (45) 520 ± 12 (47) 537 ± 12 (50) 517 ± 13 (48) 533 ± 15 (43) 558 ± 16 (47) 573 ± 13 (44) 540 ± 13 (43) 546 ± 13 (46) 537 ± 14 (47) 550 ± 15 (39) 571 ± 18 (44) 580 ± 13 (39) 552 ± 14 (41) 561 ± 14 (44) 556 ± 16 (45) 552 ± 14 (35) 570 ± 19 (36) 598 ± 14 (40) 568 ± 15 (39) 574 ± 15 (41) 568 ± 16 (41) 575 ± 16 (35) 576 ± 18 (34) 619 ± 15 (39) 575 ± 15 (35) 586 ± 18 (36) 580 ± 17 (38) 589 ± 16 (35) 588 ± 21 (31) 633 ± 18 (32) 601 ± 18 (30) 605 ± 19 (34) 591 ± 19 (36) 604 ± 19 (31) 595 ± 22…”

mentioning

confidence: 99%

NTP Research Report on the CLARITY-BPA Core Study: A Perinatal and Chronic Extended-Dose-Range Study of Bisphenol A in Rats

2018

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Comparison of endpoints relevant to toxicity assessments in 3 generations of CD-1 mice fed irradiated natural and purified ingredient diets with varying soy protein and isoflavone contents

Cited by 15 publications

References 78 publications

Impact of genistein on the gut microbiome of humanized mice and its role in breast tumor inhibition

Impact of genistein on the gut microbiome of humanized mice and its role in breast tumor inhibition

Semi‐synthetic diet versus diet using natural ingredients—Comparative study in female Golden Syrian hamsters

NTP Research Report on the CLARITY-BPA Core Study: A Perinatal and Chronic Extended-Dose-Range Study of Bisphenol A in Rats

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