Comparison of Endoscopy-Based and Serum-Based Methods for the Diagnosis ofHelicobacter pylori

Abstract

Testing for serological IgG antibodies to H pylori WC was the best to assess whether infection by H pylori was present. Neither the IgA WC nor the IgG CagA ELISAs add significant value in the diagnosis of H pylori.

Utility of diagnostic tests for detection of Helicobacter pylori in children in northeastern Mexico

“…Enzyme‐linked immunosorbent assay (ELISA) was used to study the presence of IgG antibodies in serum samples by a method validated using local H. pylori strains for the antigen preparation 22 . Data were analyzed using Benchmark Reader (Biorad, Hercules, CA, USA).…”

High Frequency of Gastric Colonization with MultipleHelicobacter pyloriStrains in Venezuelan Subjects

“…The presence of immunoglobulin G (antibodies to an H. pylori whole-cell (WC) antigen preparation or to CagA protein was assessed by enzyme-linked immunosorbent assays (ELISA), as described previously (28,39). In brief, each antigen was dissolved in 0.5 M sodium bicarbonate (pH 9.6) and used to coat microtiter plates overnight at room temperature.…”

Diagnosis of Helicobacter pylori infection

“…In a French study, serology and UBT showed positive results significantly more often than culture, histology and the HpSA test in elderly hospitalized patients with severe atrophic corpus gastritis [58]. Also among Mexican adults, invasive diagnostic methods gave positive results less often in patients with atrophic gastritis than in those without atrophic changes [59]. Furthermore, as epidemiological studies are concerned, serology might give the most reliable estimate of the association between H. pylori and the late sequelae of the infection, such as gastric cancer [60].…”