Comparison of endometrial regenerative cells and bone marrow stromal cells

Abstract

BackgroundEndometrial regenerative cells (ERC) and bone marrow stromal cells (BMSC) are being used in clinical trials. While they have been reported to have similar characteristics, they have not been directly compared.MethodsWe compared micro RNA (miRNA) and gene expression profiles, soluble cytokine and growth factor levels and ability to inhibit ongoing mixed leukocyte reaction (MLR) of ERC and BMSC each derived from 6 healthy subjects.ResultsERC and BMSC miRNA and gene expression profiles were similar, but not identical; more differences were noted in the expression of genes than in miRNAs. Genes overexpressed in ERCs were more likely to be in immune and inflammation pathways and those overexpressed in BMSCs were more likely to be in stem cell and cancer signaling pathways. In addition, the levels of IL-8 and ICAM-1 were greater in ERC supernatants while the levels of HGF, VEGF, IL-6, CXCL12, TGFB1 and TGFB2 were greater in BMSC supernatants. Additionally, ERC demonstrated greater inhibition of the proliferation of mixed leukocyte cultures.ConclusionsThese results suggest that the in vivo effects of ERC and BMSC may differ. Multiple properties of stromal cells are responsible for their in vivo effectiveness and ERC may be more effective for some of the clinical applications and BMSC for others. Studies in animal models or clinical trials will be required to more fully characterize the differences between ERC and BMSC.

Unus pro omnibus, omnes pro uno: A novel, evidence-based, unifying theory for the pathogenesis of endometriosis

“…1, Fig. 2) [123]. It has not been demonstrated whether the transplanted cells retain the characteristics of stem cell, if they behave as physiological ones for endometrial cyclicity, but, above all, mechanism of physiological recruitment in the uterus has not been shown.…”

Chimeric Self-assembling Nanofiber Containing Bone Marrow Homing Peptide’s Motif Induces Motor Neuron Recovery in Animal Model of Chronic Spinal Cord Injury; an In Vitro and In Vivo Investigation

“…Human endometrial-derived stromal cells (hEnSCs) are isolated from human endometrium and are adult and multipotent stem cells that have adipo, osteo, and chondro differentiation potential [14]. In comparison to other stem cell types, they have shown some advantages that make them a considerable source in cell therapy.…”

Human bone marrow stromal cell confluence: effects on cell characteristics and methods of assessment

“…Gene and microRNA expression analysis was performed by procedures as reported previously 37, 38 . Raw data were uploaded into mAdb database (http://madb.nci.nih.gov/) and then analyzed by BRB-ArrayTools 39 (http://linus.nci.nih.gov/BRB-ArrayTools.html) and Partek Genomics Suite (Partek Inc, Saint Louis, MO).…”