Comparison of Embryogenic Callus Induction from Two Malaysian Upland Rice Seed (<i>Cv.</i> Hitam and Wai)

Mohammed, Sulaıman; Samad, Azman Abd; Rahmat, Zaidah

doi:10.1166/asl.2018.11461

Cited by 1 publication

(3 citation statements)

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“…In monocotyledonous plants, the use of auxins as growth regulator or promoters of cell indifferentiation is absolutely possible. These enhances induction of indirect somatic embryo-genesis as reconfirmed by Meneses, Flores [20], Karthikeyan, Pandian [19], [31]. Auxins have significant effect by inducing embryogenic competence on the scutellar cells of rice seeds, leaves and other organs.…”

Section: 4-dichlorophenoxyacetic Acidmentioning

confidence: 77%

“…Trejo-Tapia, Amaya [34] Previous reports indicated that embryogenic calli production from indica sub-species face a major set-back due to its low tissue response [24,40]. But recent evidences demonstrated that callus induction or indirect somatic embryogenesis from matured seeds of upland rice is persuade by 2,4-D at different concentrations [31]. Thus, the concentration of the hor-mone used, the culture period and sub-culture time control the responses and virtually recon-version of embryos-to plants.…”

Section: Plant Growth Regulators (Pgr) and Their Classificationmentioning

confidence: 99%

“…Precise media manipulations can be practice to direct embryo-genic callus development in culture. Manipulation or optimization of medium are either from carbon source, amino acids basis, vitamin or growth regulators [31].…”

Section: Plant Growth Regulators (Pgr) and Their Classificationmentioning

confidence: 99%

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Effects and Quantıty Ranges of Some Auxıns on Embryogenıc Callus Inductıon From Upland Rıce Cultıvars: An Overview

Mohammed

2020

International Journal of Life Sciences and Biotechnology

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Embryogenic callus induction from indica rice sub-species, upland cultivars has remain a challenging task, but plant growth regulators (PGRs) have been found to play a vital role in enhancing the production. Hence, solving this tricky problem and achieving optimum embryogenic callus induction from this special type of rice is a welcome development as a food security concern, while proper examination of the appropriate PGRs is the only solution. Here we reviewed on two auxins-PGRs that promote the embryogenic callus induction from upland rice, viz., 2,4-D and NAA with their recommendable quantity ranges. 2,4-D and NAA were the most commonly employed and discovered best growth hormones in PTC irrespective of explant in all cereal plants. They are found the most suitable hormones and positively upgrade the production of reproducible callus. Classical analyses implies that medium supplemented with such PGRs provides necessities for quality callus from upland rice. Evidences indicated that callus induction or somatic embryogenesis from matured seeds of upland rice is persuade by 2,4-D between 1.0-2.5 mg/L concentrations. Whereas for NAA, to obtain its desired effects on upland rice callus induction it must be fortified in concentrations ranging from 0.5 mg/L to 10 mg/L. But convincingly, 0.5-5.0 mg/L NAA with other PGR like 2,4-D would be more proper. Hence, extensive studies of these hormones and others will provide insights on their potentialities to embryogenic callus induction and probably identify more of their roles in PTC analysis.

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Section: 4-dichlorophenoxyacetic Acidmentioning

confidence: 77%

Section: Plant Growth Regulators (Pgr) and Their Classificationmentioning

confidence: 99%