Comparison of embryo quality between intracytoplasmic sperm injection and in vitro fertilization in sibling oocytes

Abstract

Background The aim of the present study was to compare the quality of embryos derived from sibling oocytes by in vitro fertilization (IVF) or ICSI. Methods Consecutive patients with a less than 40% fertilization rate in a previous standard IVF cycle or with relative male factor infertility were recruited for the study. The oocytes retrieved from each patient were divided into two groups for either conventional insemination (group A) or ICSI (group B). Power analysis showed that to detect a 25% difference in the rate of high-quality embryos between the groups with a power of 0.8, at least 1,200 oocytes were needed in each group. Results One hundred seventy-seven patients were included in the study. Group A was comprised of 1,526 oocytes and group B of 1,480 sibling oocytes. As expected, the fertilization rate was significantly higher in group B than group A (67.1 vs. 43.6%, p < 0.001). No significant between-group differences were observed in cleavage rate (92.7 and 89.7%, respectively) and the rate of either grade A embryos (22.6 and 23.9%, respectively) or grade A 1 embryos (37.3 and 33.5%, respectively).However, in the subgroup of patients with relative malefactor infertility (n=36), the rate of grade A 1 embryos was significantly higher in the IVF than the ICSI group (46.4 vs. 29.0%, respectively, p=0.02). Conclusions Embryo quality does not seem to be influenced by the mode of fertilization (IVF or ICSI). We assume that embryo quality depends on intrinsic factors of the gametes involved rather than on the fertilization process per se.

Oocyte insemination techniques are related to alterations of embryo developmental timing in an oocyte donation model

“…When this work compared embryo quality and implantation potential from a kinetic point of view, the results demonstrated no significant differences between the IVF and ICSI groups, which is agreement with previous studies (Ruiz et al, 1997;Yoeli et al, 2008), although IVF-derived embryos showed a positive trend in the percentages of optimal embryos with a predicted higher implantation potential. Thus, after controlling for maternal effects, embryo morphokinetic characteristics do not seem to be influenced by the mode of fertilization, so it is possible to assume that embryo quality depends on intrinsic factors of the gametes involved rather than on the fertilization process per se.…”

Comparison of in vitro fertilization versus intracytoplasmic sperm injection in extremely low oocyte retrieval cycles

“…Dumoulin et al (18) demonstrated that embryos obtained after ICSI had a decreased potential to develop into blastocysts. Yoeli et al (19) and our previous report (20,21) also revealed that embryo quality does not seem to be influenced by the mode of fertilization (IVF or ICSI). We may assume that embryo quality depends on intrinsic factors of the gametes involved, rather than on the fertilization process per se.…”

Live birth rates using conventional in vitro fertilization compared to intracytoplasmic sperm injection in Bologna poor responders with a single oocyte retrieved

“…In patients with very low numbers of oocytes retrieved and non-male factor infertility, conflicting data have been reported, with either significantly higher fertilization rates using ICSI as compared to IVF [32], or similar between the two methods [31]. In both of the above studies, clinical pregnancy, implantation rates, live birth rates, cycle cancellation and early pregnancy loss did not differ between the two fertilization methods [31,32], suggesting that embryo quality does not seem to be affected by the method of fertilization, but rather by the intrinsic quality of the gametes themselves [46].…”