Comparison of ELISA with virus isolation for the diagnosis of genital herpes.

Alexander, I Archakov; Ashley, C. R.; Smith, K. J.; Harbour, J.; Roome, A. P. C. H.; Darville, J. M.

doi:10.1136/jcp.38.5.554

Cited by 12 publications

(5 citation statements)

References 12 publications

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“…The use of selected specimens may partly explain the wide variation in antigen detection rate achieved by different groups [Vestergaard and Jensen, 1981 ;Adler Storthz et al, 1983;Grillner and Landqvist, 1983;Lawrence et al, 1984;Morgan and Smith, 1984;Nerurkar et al, 1984;Warford et al, 1984;Alexander et al, 1985;Clayton et al, 19851. For this reason it is prudent to use the same panel of unselected specimens for comparative evaluation studies.…”

Section: Discussionmentioning

confidence: 96%

“…It was interesting to find that antigen detection rates varied considerably when specimens were selected on the basis of two clinical parameters, being high with penile vesicular lesions (25/27, 93%) and low with vulva1 erosions (20/35, 57%). The use of selected specimens may partly explain the wide variation in antigen detection rate achieved by different groups [Vestergaard and Jensen, 1981 ;Adler Storthz et al, 1983;Grillner and Landqvist, 1983;Lawrence et al, 1984;Morgan and Smith, 1984;Nerurkar et al, 1984;Warford et al, 1984;Alexander et al, 1985;Clayton et al, 19851. For this reason it is prudent to use the same panel of unselected specimens for comparative evaluation studies.…”

Section: Discussionmentioning

confidence: 99%

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Herpes Simplex Virus Detection by ELISA: Effect of Enzyme Amplification, Nature of Lesion Sampled and Specimen Treatment

Clayton

Roberts

Chantler

et al. 1986

Journal of Medical Virology

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The relative sensitivity of two enzyme detection procedures was investigated in a simultaneous "monoclonal" ELISA for herpes simplex virus (HSV). A cyclical enzyme amplified detection system with alkaline phosphatase, rather than horse-radish peroxidase and a conventional chromogenic substrate, gave an increase in absolute sensitivity and a 20 to 30% increase in the detection of HSV in routine isolation-positive genital specimens collected in transport medium. The HSV detection rate, with both procedures, was shown to vary with the site and clinical stage of lesion sampled; it was highest with penile vesicular lesions. Direct extraction of the swab specimen in a small volume of diluent further increased the sensitivity of antigen detection giving positive and negative predictive values of 100 and 96% respectively. The overall sensitivity of HSV detection was equivalent to that obtained by isolation in cell culture. The amplified ELISA offers an alternative, rapid, simple, non-culture technique for routine HSV diagnosis that does not rely upon retention of virus viability.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Herpes Simplex Virus Detection by ELISA: Effect of Enzyme Amplification, Nature of Lesion Sampled and Specimen Treatment

Clayton

Roberts

Chantler

et al. 1986

Journal of Medical Virology

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“…The sensitivity of EIA appears to be lowest (approximately 34%) when it is performed on cervical specimens obtained from asymptomatic females (94,95). In contrast, when EIA is applied to the testitig of symptomatic patients with suspicious clinical lesions, a sensitivity of 84% has been demonstrated (93).…”

Section: Antigen-detection Techniquesmentioning

confidence: 97%

Rapid Diagnosis of Common Sexually Transmitted Diseases in Adolescents: A Review

Krowchuk

Anglin

Kumar

1989

Pediatric Dermatology

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Sexually transmitted diseases (STDs) represent a significant health problem for adolescents in the United States. As a result, clinicians from a number of disciplines, among them pediatrics and dermatology, may be called on to evaluate teenage patients with these infections. Traditionally, the diagnosis of many STDs has relied on isolation of the causative organisms in culture. While such procedures typically offer optimal diagnostic accuracy, they are often time consuming and expensive, and may not be widely available. In an attempt to remedy this, culture-independent techniques have been developed to permit more rapid and economical diagnosis of these important pathogens. The strengths and limitations of these methods must be understood, however, for the tests to be used effectively.

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“…The crux of ELISA lies in its capacity to detect and quantify the antibody-antigen binding event through the enzyme's catalytic activity. This catalysis results in the conversion of a reagent into a discernible signal, which can be harnessed to estimate the concentration of the antigen (as illustrated in Figure 3) (74)(75)(76). Horseradish peroxidase (HRP) is a commonly used enzyme in ELISA setups due to its ability to amplify signals and enhance assay sensitivity (31).…”

Section: 29mentioning

confidence: 99%

Virus Quantification Methods with Focus on Marek's Disease Viruses

Piriaei,

Razmyar,

Dolatyabi

et al. 2023

J Poult Sci Avian Dis

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Marek's disease (MD) is a significant concern in the poultry industry, causing neoplastic disease and substantial economic losses. MDV-1, MDV-2, and MDV-3 are the three serotypes of the herpesvirus that cause MD, each with unique traits and interactions with the host. Vaccination is the primary preventive measure, but some vaccinated flocks still experience losses, often due to suboptimal vaccine dosages. Accurate quantification of the virus is crucial for understanding its dynamics, evaluating vaccine efficacy, and ensuring the health and productivity of poultry populations. Virus quantification methods fall into two main categories: infectivity assays and chemical/physical assays. Infectivity assays detect active, infectious virions, such as plaque, focus formation, and endpoint dilution. Chemical/physical assays, including the hemagglutination assay, transmission electron microscopy, flow cytometry, and qPCR, target specific components of the virion. However, these methods cannot distinguish between infectious and inactivated virions, potentially leading to overestimating viable viral populations. Marek's disease viruses (MDVs) can be quantified using in vitro plaque assays in susceptible cell cultures. The viral plaque assay determines the number of plaque-forming units (pfu) in a virus sample. Titration procedures for vaccine strains are similar to those for pathogenic variants. It is crucial to assess the dose of reconstituted MD vaccine (in PFU per chicken) to identify potential vaccine inefficacies and prevent outbreaks in the field. Accurate virus quantification is pivotal in understanding viral kinetics, optimizing therapeutic interventions, evaluating vaccine effectiveness, and preventing Marek's disease outbreaks in poultry.

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Comparison of ELISA with virus isolation for the diagnosis of genital herpes.

Cited by 12 publications

References 12 publications

Herpes Simplex Virus Detection by ELISA: Effect of Enzyme Amplification, Nature of Lesion Sampled and Specimen Treatment

Herpes Simplex Virus Detection by ELISA: Effect of Enzyme Amplification, Nature of Lesion Sampled and Specimen Treatment

Rapid Diagnosis of Common Sexually Transmitted Diseases in Adolescents: A Review

Virus Quantification Methods with Focus on Marek's Disease Viruses

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