Comparison of ELISA and immunoblotting techniques for the detection of cherry mottle leaf virus

James, D.; Mukerji, S.

doi:10.1111/j.1744-7348.1996.tb05727.x

Cited by 9 publications

(1 citation statement)

References 17 publications

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“…The increased sensitivity allows greater confidence in the results of testing for both certification and quarantine applications, and also allows greater flexibility in the timing of testing and in the type of tissue that may be used to detect the pathogen of interest. Detection of some pathogens using traditional techniques that are serology-based is often limited to certain months of the year and to certain parts of the plant (72). PCR detection facilitates reliable testing at any time of year since the sensitivity of the test allows detection of the pathogen in most tissue including dormant woody material (71,173).…”

Section: Sensitivity and Duration Of Testingmentioning

confidence: 99%

Impacts of Molecular Diagnostic Technologies on Plant Disease Management

Martín

James

Lévesque

2000

Annu. Rev. Phytopathol.

216

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Detection and diagnosis of plant viruses has included serological laboratory tests since the 1960s. Relatively little work was done on serological detection of plant pathogenic bacteria and fungi prior to the development of ELISA and monoclonal antibody technologies. Most applications for laboratory-based tests were directed at virus detection with relatively little emphasis on fungal and bacterial pathogens, though there was some good work done with other groups of plant pathogens. With the advent of molecular biology and the ability to compare regions of genomic DNA representing conserved sequences, the development of laboratory tests increased at an amazing rate for all groups of plant pathogens. Comparison of ITS regions of bacteria, fungi, and nematodes has proven useful for taxonomic purposes. Sequencing of conserved genes has been used to develop PCR-based detection with varying levels of specificity for viruses, fungi, and bacteria. Combinations of ELISA and PCR technologies are used to improve sensitivity of detection and to avoid problems with inhibitors or PCR often found in plants. The application of these technologies in plant pathology has greatly improved our ability to detect plant pathogens and is increasing our understanding of, their ecology and epidemiology.

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Section: Sensitivity and Duration Of Testingmentioning

confidence: 99%