Comparison of electrophoresis, isoelectric focusing, and immunodiffusion in determinations of cow's and goat's milk in Serra da Estrela cheeses

Major whey proteins in ovine and caprine acid wheys from indigenous greek breeds

“…Both b-LG and a-LA IEF patterns consisted of one major band and some other minor faint bands that could be attributed to their glycosylated forms similarly to the ovine patterns. The caprine b-LG was focused at pI 5.9, that was close to pI value of 6.06 found by Amigo, Ramos, Calhau, and Barbosa (1992) and equal to pI 5.9 of Pintado and Malcata (1999). The isoelectric point of caprine a-LA was estimated as pI 5.2 that was equal to that estimated for ovine a-LA in the present study and similar to pI 5.4 reported by Pintado and Malcata (1999).…”

CONTRIBUTION TO THE GENETIC DETERMINISM OF THE SHEEP’S MILK QUALITY. RACE INFLUENCE (HAMRA AND OULED DJELLAL) ON PHYSICO-CHEMICAL PARAMETERS AND PROTEIN PROFILE

“…Such observations are also reported by (Amigo et al, 1992), which locate characteristic bands corresponding to bovine alphalactalbumin and beta-lactoglobulin further forward than its ovine counterpart.…”

“…The fraction of β-Lg in our samples appear as a single intense band with the similar electrophoretic mobility to the bovine α-La. Similar observations were made by (Amigo et al, 1992;Blitzsteina and Diaconisb, 2011) which located and indicated as in the bovine counter part the existence of two genetic variants of ovine β-Lg but could not be distinguished.…”

A TaqMan real-time PCR system for the identification and quantification of bovine DNA in meats, milks and cheeses

“…Adulteration of goat and water buffalo milk and their products by cows' milk is therefore economically attractive. Their products are traditionally tested for adulteration by immunological and/or electrophoretic methods (Amigo, Ramos, Calhau, & Barbosa, 1992;Cerquaglia & Avellini, 2004;Hurley, Ireland, Coleman, & Williams, 2004;Levieux & Venien, 1994;Mayer, 2005;Mimmo & Pagani, 1998). Identification of milk by PCR amplification of DNA is based on the presence of mammalian somatic cells in the milk (Herman, 2001).…”