Comparison of electrochemical detection of acetylcholine-induced nitric oxide release (NO) and contractile force measurement of rabbit isolated carotid artery endothelium

Kılınc, Emrah; Yetik, Günay; Dalbastı, Tayfun; Ozsoz, Mehmet

doi:10.1016/s0731-7085(01)00621-5

Cited by 12 publications

(2 citation statements)

References 31 publications

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“…Some of the reported HPLC methods were based on the conversion of nitrate to nitrite or the derivatization of nitrite and nitrate with other reagents [ 6 , 7 , 11 ]. However, their sample preparations were complicated.…”

Section: Discussionmentioning

confidence: 99%

“…Extensive interests have been shown to determine nitrite and nitrate in biological fluids. The methods include Griess reaction [ 5 ], chemiluminescence [ 6 ], fluoelectrochemical probes [ 7 ], spectrophotometry [ 8 ], electron paramagnetic resonance [ 9 ], electrophoresis [ 10 ] high-performance liquid chromatography [ 11 ], gas chromatography [ 12 ], and ion chromatography [ 13 ]. Among these reported methods, the HPLC methodology with the advantages of high sensitivity was widely applied.…”

Section: Introductionmentioning

confidence: 99%

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Determination of Nitric Oxide-Derived Nitrite and Nitrate in Biological Samples by HPLC Coupled to Nitrite Oxidation

Duan

Tang

et al. 2013

Chromatographia

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Nitrite and nitrate are main stable products of nitric oxide, a pivotal cellular signaling molecule, in biological fluids. Therefore, accurate measurement of the two ions is profoundly important. Nitrite is difficult to be determined for a larger number of interferences and unstable in the presence of oxygen. In this paper, a simple, cost-effective and accurate HPLC method for the determination of nitrite and nitrate was developed. On the basis of the reaction that nitrite is oxidized rapidly to nitrate with the addition of acidic potassium permanganate, the determination of nitrite and nitrate was achieved by the following strategy: each sample was injected twice for HPLC analysis, i.e. the first injection was to measure nitrate, and the second injection was to measure total nitrate including initial nitrate and the nitrate from the conversion of nitrite with the addition of acid potassium permanganate in the sample. The amount of nitrite can be calculated as difference between injections 2 and 1. The HPLC separation was performed on a reversed phase C18 column for 15 min. The mobile phase consisted of methanol–water (2:98 by volume); the water in the mobile phase contained 0.60 mM phosphate salt (potassium dihydrogen and disodium hydrogen phosphate) and 2.5 mM tetrabutylammonium perchlorate (TBAP). The UV wavelength was set at 210 nm. Additionally, we systemically investigated the effects of the concentration of phosphate salt and TBAP in the mobile phase, the pH of the mobile phase, and the amount of acidic potassium permanganate added to the sample on the separation efficacy. The results showed that the limits of detection (LOD) and the limit of quantitation (LOQ) were 0.075 and 0.25 μM for nitrate (containing the oxidized nitrite), respectively. The linear range was 1–800 μM. This developed approach was successfully applied to assay nitrite/nitrate levels in cell culture medium, cell lysate, rat plasma and urine.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Determination of Nitric Oxide-Derived Nitrite and Nitrate in Biological Samples by HPLC Coupled to Nitrite Oxidation

Duan

Tang

et al. 2013

Chromatographia

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Electrochemical Nitric Oxide Sensors for Biological Samples – Principle, Selected Examples and Applications

2003

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The discoveries made in the 1980s that NO could be synthesized by mammalian cells and could act as physiological messenger and cytotoxic agent had elevated the importance of its detection. The numerous properties of NO, that enable it to carry out its diverse functions, also present considerable problems when attempting its detection and quantification in biological systems. Indeed, its total free concentration in physiological conditions has been established to be in nanomolar range. Thus, detection of nitric oxide remains a challenge, pointing out the difficult dual requirements for specificity and sensitivity. Exception made for the electrochemical techniques, most of the approaches (namely UV-visible spectroscopy, fluorescence, electron paramagnetic resonance spectroscopy) use indirect methods for estimating endogenous NO, relying on measurements of secondary species such as nitrite and nitrate or NO-adducts. They also suffer from allowing only ex situ measurements. So, the only strategies that allow a direct and in vivo detection of NO are those based on the use of ultramicroelectrodes. The reality is that surface electrode modification is needed to make the ultramicroelectrode material selective for NO. Therefore, the design of modified electrode surfaces using organized layers is very attractive and provides the ideal strategy. This review addresses a global description of the various approaches that have involved chemically modified microelectrodes specially designed for the electrochemical detection of NO in biological media. Selected significant examples of applications in biological tissues are also reported in order to highlight the importance of this approach in having new insights into the modulatory role of NO in physiology and pathophysiology.

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Electrochemical sensor for superoxide anion radical using polymeric iron porphyrin complexes containing axial 1‐methylimidazole ligand as cytochrome c mimics

Yuasa

Oyaizu

Yamaguchi

et al. 2005

Polymers for Advanced Techs

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A needle-type electrochemical sensor for the facile detection of superoxide anion radical (O 2 À.) was devised using an electrodeposited film of a polymeric porphyrin complex attached to a carbon microelectrode which was placed in a stainless steel 18G needle tube as an auxiliary electrode. The sensing element was prepared by means of electropolymerization of bromoiron(III) mesotetra(3-thienyl)porphyrin in the presence of 1-methylimidazole in CH 2 Cl 2 containing 100 mM tetrabutylammonium perchlorate (TBAP) as a supporting electrolyte, which gave a smooth film of the corresponding polymer. The film was electrochemically active to give a redox response near À0.05 V versus Ag/AgCl due to the iron(II/III) couple. The microsensor was applied to detect O 2 À. produced by xanthine oxidation catalyzed by xanthine oxidase. The amperometric response for O 2 À. was monitored at an electrode potential of 0.5 V versus the auxiliary electrode in a 10 mM phosphate buffer. The microsensor displayed a high catalytic activity for the oxidation of O 2 À.and showed a linear relationship between the current and the O 2 À. concentration. Axial coordination of an imidazole ligand to the iron porphyrin center enhanced selectivity for O 2 À. by impeding the undesired coordination of H 2 O 2 that resulted from the dismutation of O 2 À..

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Comparison of electrochemical detection of acetylcholine-induced nitric oxide release (NO) and contractile force measurement of rabbit isolated carotid artery endothelium

Cited by 12 publications

References 31 publications

Determination of Nitric Oxide-Derived Nitrite and Nitrate in Biological Samples by HPLC Coupled to Nitrite Oxidation

Determination of Nitric Oxide-Derived Nitrite and Nitrate in Biological Samples by HPLC Coupled to Nitrite Oxidation

Electrochemical Nitric Oxide Sensors for Biological Samples – Principle, Selected Examples and Applications

Electrochemical sensor for superoxide anion radical using polymeric iron porphyrin complexes containing axial 1‐methylimidazole ligand as cytochrome c mimics

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