Comparison of elastic properties of tissue samples in various pathological states using optical coherence elastography

Gubarkova, Ekaterina V.; Sovetsky, Alexander A.; Zaitsev, Vladimir Yu.; Matveev, Lev A.; Matveyev, Alexander L.; Vorontsov, Dmitry А.; Timofeeva, Lidia B.; Kiseleva, Elena B.; Vorontsov, А. Yu.; Kuznetsova, Irina A.; Gladkova, Natalia D.

doi:10.1117/12.2521529

Cited by 3 publications

(5 citation statements)

References 12 publications

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“…However, the structural OCT image does not reflect the true state of the vaginal wall submucosal connective tissue during POP and does not allow for the distinguishing of it from the age norm. The obtained results are consistent with the results of earlier studies suggesting a distinct decrease in the elastic modulus of tissues in POP compared to normal tissue [65,73]. However, in this study we demonstrate for the first time what specific changes in the collagen bundles of vaginal connective tissue are associated with a decrease in its elastic properties in the early stages of POP.…”

Section: Comparative Visualsupporting

confidence: 93%

“…Furthermore, the emerging OCT-based modalities, such as OCE and possibilities of objective quantitative analysis of the OCT data in combination with real-time imaging, additionally extend the variety of clinical applications of OCT methods. In an earlier study, our group tested the application of C-OCE technology to evaluate the vaginal wall state using postoperative samples of patients with stage IV prolapse [65]. In this pilot study, we demonstrated significant differences not only in the values of Young's modulus, but also in the character of elastic responses in age norm vaginal wall and stage IV prolapse.…”

Section: Discussionmentioning

confidence: 87%

“…The data obtained in different measurements could be meaningfully compared due to utilization of the developed procedure of the applied stress standardization using the reference silicone layers as optical stress sensors [58,64]. Without such standardization, the intrinsic elastic nonlinearity of vaginal wall tissues may result in uncontrollable variability of the estimated elastic modulus in different measurements, and even different parts of the same image [65]. For the quantitative estimates of tangent Young's modulus presented below, in all measurements, we used the stress range from 1 kPa to 3 kPa centered at 2 kPa.…”

Section: Compression Oce Imagingmentioning

confidence: 99%

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Compression Optical Coherence Elastography for Assessing Elasticity of the Vaginal Wall under Prolapse after Neodymium Laser Treatment

et al. 2022

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Early stages of pelvic organ prolapses are mainly associated with the pelvic floor disfunction as a result of elasticity changes in the connective tissues including the vaginal wall. In this study, for the first time we used a compression optical coherence elastography (C-OCE) method for assessing elasticity of the vaginal wall under prolapse conditions after intravaginal neodymium (Nd:YAG) laser treatment. C-OCE was used for a comparative ex vivo study of vaginal wall average values of stiffness (elastic Young’s modulus) in patients with age norm (n = 6), stage I–II prolapse (n = 5) without treatment and stage I–II prolapse post 1–2 months Nd:YAG laser treatment (n = 10). To verify the C-OCE data, the structural features of the submucosal connective tissue were identified morphometrically by Van Gieson staining using quantitative textural analysis of the state of collagen bundles. The results of a comparative evaluation of C-OCE and histological images demonstrate a statistically significant tissue stiffness decrease in vaginal wall prolapse compared to the age norm (73.5 ± 18.9 kPa vs. 233.5 ± 48.3 kPa; p < 0.05). This agrees with the histologically revealed increase in the space between the bundles of collagen fibers, which leads to a decrease in the uniformity of their arrangement. After Nd:YAG laser treatment, we observed statistically significant connective tissue stiffness increase compared to vaginal wall prolapse without treatment (152.1 ± 19.2 kPa vs. 73.5 ± 18.9 kPa; p < 0.05), which was associated with an increase in the local thickness of the collagen bundles, a change in their orientation, and an increase in the uniformity of their arrangement. The obtained results indicate that the C-OCE can be a robust method for detecting the early stages of vaginal wall prolapse and assessing the elastic modulus increase in the vaginal wall after laser treatment.

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Section: Comparative Visualsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 87%

Section: Compression Oce Imagingmentioning

confidence: 99%

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Compression Optical Coherence Elastography for Assessing Elasticity of the Vaginal Wall under Prolapse after Neodymium Laser Treatment

et al. 2022

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“…A recent trend in C‐OCE is its development beyond the conventionally assumed linear‐elasticity paradigm. The ability of C‐OCE to obtain nonlinear stress‐stain curves in a fairly wide strain range 64–70 is a unique advantage of C‐OCE differing it from other OCE variants. This “nonlinear trend” in C‐OCE is still emerging, but it has already demonstrated interesting, inaccessible to linear methods diagnostic prospects 71 .…”

Section: Discussionmentioning

confidence: 99%

“…Figure 18B shows such a nonlinear stress–strain curve for a breast‐cancer sample, for which the slope of the stress–strain curve (ie, the current Young's modulus varies over three times within a rather moderate strain range ~ 2.5%). Numerous other examples for various tissues and various states of the same tissue type can be found in) 65–70 …”

Section: Quantitative Stiffness Mapping In Ocementioning

confidence: 99%

Strain and elasticity imaging in compression optical coherence elastography: The two‐decade perspective and recent advances

Zaitsev

Matveyev

Matveev

et al. 2020

Journal of Biophotonics

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108

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Quantitative mapping of deformation and elasticity in optical coherence tomography has attracted much attention of researchers during the last two decades. However, despite intense effort it took ~15 years to demonstrate optical coherence elastography (OCE) as a practically useful technique. Similarly to medical ultrasound, where elastography was first realized using the quasi‐static compression principle and later shear‐wave‐based systems were developed, in OCE these two approaches also developed in parallel. However, although the compression OCE (C‐OCE) was proposed historically earlier in the seminal paper by J. Schmitt in 1998, breakthroughs in quantitative mapping of genuine local strains and the Young's modulus in C‐OCE have been reported only recently and have not yet obtained sufficient attention in reviews. In this overview, we focus on underlying principles of C‐OCE; discuss various practical challenges in its realization and present examples of biomedical applications of C‐OCE. The figure demonstrates OCE‐visualization of complex transient strains in a corneal sample heated by an infrared laser beam.

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Full-optical method of local stress standardization to exclude nonlinearity-related ambiguity of elasticity estimation in compressional optical coherence elastography

et al. 2020

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Pronounced elastic nonlinearity intrinsic to many biological tissues makes the Young's modulus (stiffness) of the tissue strongly dependent on the current strain and stress. The measured modulus may vary several times for rather moderate tissue straining within a few per cent, such strains being quite typical for compressional optical coherence elastography (C-OCE). This pronounced stiffness variability may strongly affect diagnostic conclusions based on the stiffness measurements, so that obtaining reproducible estimates of stiffness in C-OCE is an important and challenging problem. Here, we report a novel full-optical method allowing one to ensure a preselected stress level in C-OCE stiffness mapping. The proposed improved OCE visualization enables reproducible quantitative elastographic visualization even for strongly elastically-nonlinear biological tissues, which is of key importance for reliability of diagnostic applications of C-OCE. In particular, in oncology such OCE technique can be used to control clear tumor resection boundary, express assessment of tumor subtypes and in vivo monitoring of morphological alterations in animal tumor models in response to anti-tumor therapies.

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Comparison of elastic properties of tissue samples in various pathological states using optical coherence elastography

Cited by 3 publications

References 12 publications

Compression Optical Coherence Elastography for Assessing Elasticity of the Vaginal Wall under Prolapse after Neodymium Laser Treatment

Compression Optical Coherence Elastography for Assessing Elasticity of the Vaginal Wall under Prolapse after Neodymium Laser Treatment

Strain and elasticity imaging in compression optical coherence elastography: The two‐decade perspective and recent advances

Full-optical method of local stress standardization to exclude nonlinearity-related ambiguity of elasticity estimation in compressional optical coherence elastography

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