Comparison of Effects of Stimulation Days on Oocyte Morphology and Day 5 Blastocyst Rate in ICSI Cycle

Abstract

Aim: To compare and identify the effect of normal and prolonged stimulation on oocyte morphology and day 5 blastocyst rate in intracytoplasmic sperm injection (ICSI) cycle in normal reserve patients. Materials and methodology:A comparative study where two groups were considered-patients undergoing controlled ovarian stimulation for ICSI cycle with anti-müllerian hormone (AMH) 2-4 ng/mL with stimulation days of 10 and 11, and patients undergoing controlled ovarian stimulation for ICSI cycle with AMH 2-4 ng/mL with stimulation days of 12, 13, and 14 between March 2023 and May 2023. The mean AMH value was 2.91 ng/mL. The mean age of the patients in normal stimulation was 31.25 years and in prolonged stimulation was 33.11 years. Around 34 patients (403 oocytes) were analyzed in normal stimulation and 32 patients (303 oocytes) were analyzed in prolonged stimulation. Type of trigger, gonadotropin used, and the dosage were not considered. Result: The oocyte maturation rate, fertilization rate, cleavage rate, day 3 good embryo rate, and day 5 blastocyst rate of normal stimulation are 76, 82, 76, 61, and 39%, respectively. The oocytes with single, double, and multiple defects of normal stimulation are 23, 55, and 22%, respectively. The oocyte maturation rate, fertilization rate, cleavage rate, day 3 good embryo rate, and day 5 blastocyst rate of prolonged stimulation are 76, 84, 76, 64, and 30%, respectively. The oocytes with single, double, and multiple defects of normal stimulation are 6, 41, and 77%, respectively. The p-value for the oocyte maturation rate was 0.032535731 and the p-value for day 5 blastocyst rate was 0.003925882. Conclusion: Prolonged stimulation is associated with oocyte maturation rate, oocyte morphology, and day 5 blastocyst rate.