Comparison of effects of parathyroidectomy and ethane-1-hydroxy-1,1-diphosphonate (EHDP) administration upon bone synthesis of hydroxyproline and urinary excretion of hydroxyproline in rats

Biochemical aspects of biomineralization

“…Collagen catabolism is depressed to a greater extent than collagen synthesis s47). These findings are in accord with other investigations s3s' s42, [545][546][547]. Osteoclast population parameters are increased with both C12MDP and EHDP with CI2MDP having a greater effect than similar doses of EHDP s48).…”

Effects of Dietary NaCl Supplementation on Bone Synthesis of Hydroxyproline, Urinary Hydroxyproline Excretion and Bone45Ca Uptake in the Rat

“…Urinary cyclic AMP was also measured on day 10, as an index of parathyroid function (Goulding 1980). In order to measure the bone synthesis of 3 H-hydroxyproline, rats were given labelled proline (L-proline-2,3-3 H, New England Nuclear Corporation, 30 Ci/mmol) by intraperitoneal injection (150 nCi/kg body wt in 1.0 ml 0.9% NaCI) 24 hours before they were killed (Goulding and McChesney 1977). The left femur was then taken for mineral analysis (Goulding and Campbell 1984) and the right femur for hydroxyproline measurement.…”

“…Hydroxyproline was measured by the method of Bergman and Loxley (1970). Tritiated hydroxyproline in bone was separated from 3 H-proline on Dowex X-100 as described previously (Goulding and McChesney 1977). Radioactivity was measured on an LKB Rackbeta II Model 1214 liquid scintillation counter and samples were corrected for quenching.…”