Comparison of effects of leptin and ghrelin on porcine ovarian granulosa cells

Sirotkin, Alexander V.; Meszarosova, Monika

doi:10.1016/j.domaniend.2009.06.001

Cited by 35 publications

(24 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This study thus supports previous reports showing resistin stimulate proliferation of different cell types (Ort et al, 2005;Park et al, 2008). Other adipokines, such as adiponectin and leptin, has earlier been shown to stimulate GC proliferation in different species including the bat, S. heathi (Spicer et al, 2000;Sirotkin and Grossmann, 2007;Maillard et al, 2010;Sirotkin and Meszarosova, 2010). As shown for adiponectin (Chabrolle et al, 2007) and leptin (Duggal et al, 2002), resistin failed to induced proliferation of GCs in the ovary of rat, but stimulate GCs proliferation in cow ovary (Maillard et al, 2011).…”

Section: Discussionsupporting

confidence: 92%

Effects of resistin on ovarian folliculogenesis and steroidogenesis in the vespertilionid bat, Scotophilus heathi

Singh

Suragani

Krishna

2014

General and Comparative Endocrinology

View full text Add to dashboard Cite

The bat Scotophilus heathi exhibit prolonged anovulatory condition known as delayed ovulation coinciding with the period of extensive fat accumulation. The present study was undertaken to find out whether extensive accumulation of fat in S. heathi is responsible for suppression of ovarian activity by increasing production of adipokine resistin in the bat. This was achieved by (a) investigating variation in serum resistin level in relation to the changes in the body fat mass and (b) evaluating the effect of resistin treatment on ovarian activity with reference to steroid synthesis. An attempt was also made to determine whether resistin mediate its effects on ovary through signal transducer and activator of transcription 3 (STAT3) signaling mechanism. The results showed significant seasonal variation in serum resistin level with the peak level coinciding with the period of maximum fat accumulation, high circulating androgen level and period of anovulation. The treatment with resistin to the bat caused increase in androstenedione due to stimulatory effects on 3β-hydroxysteroid dehydrogenase, but decrease in estradiol level due to inhibitory effect on aromatase. Resistin treatment increased androgen receptor protein together with increased insulin receptor but not through conventional luteinizing hormone receptor and steroidogenic acute regulatory protein mediated pathways. This study further showed that resistin treatment increases androstenedione synthesis and up-regulates insulin receptor in the ovary through STAT3 mediated pathways. These findings suggest that obese women through increased resistin synthesis may causes development of non-ovulatory antral follicles through insulin receptor signaling cascade.

show abstract

Section: Discussionsupporting

confidence: 92%

Effects of resistin on ovarian folliculogenesis and steroidogenesis in the vespertilionid bat, Scotophilus heathi

Singh

Suragani

Krishna

2014

General and Comparative Endocrinology

View full text Add to dashboard Cite

show abstract

“…Then the cells were centrifuged, resuspended and plated out at 1.5 Â 10 5 viable cells per well in a 24-well plate (total volume: 1 ml containing 10% Fetal Bovine Serum (Sigma) and Antibiotic & Antimycotic solution (Penicillin-G 100U/ml, Streptomycin 100 lg/ml, Amphotericin 0.25 lg/ml (SV30079.01; Hyclone, Thermo Scientific) in a humidified CO2 (5%) incubator at 38.5°C. The cells were allowed to attach and grow (75-80% confluent) for 48 h and there after the media was replaced with fresh media containing different concentrations (1, 10, and 100 ng/ml) of ghrelin and were maintained for 48 h. The doses of the ghrelin were selected based on the earlier report (Sirotkin et al, 2009;Sirotkin and Meszarosova, 2010). Control cells were grown in media without ghrelin.…”

Section: Genementioning

confidence: 99%

Expression and localization of ghrelin and its receptor in ovarian follicles during different stages of development and the modulatory effect of ghrelin on granulosa cells function in buffalo

Gupta

Dangi

Singh

et al. 2015

General and Comparative Endocrinology

View full text Add to dashboard Cite

“…Moreover, other adipokines, such as adiponectin and leptin, were able to modulate basal or hormone-induced proliferation of GCs from different species (Spicer et al 2000, Sirotkin & Grossmann 2007, Sirotkin et al 2008, Maillard et al 2010, Sirotkin & Meszarosova 2010. For these reasons, we evaluated the potential effects of several doses of recombinant resistinGIGF1 (76 ng/ml) on the proliferation of primary bovine and rat GCs for 24 h. As already shown for adiponectin (Chabrolle et al 2007b) and leptin (Duggal et al 2002) on rat GC proliferation, no effect of rr resistin (10 and 100 ng/ml) was observed on basal and IGF1-induced mitosis of rat GCs in our study, suggesting that adipokines including resistin do not play a role in rat GC growth.…”

Section: Role Of Resistin In Granulosa Cellsmentioning

confidence: 99%

Expression and effect of resistin on bovine and rat granulosa cell steroidogenesis and proliferation

Maillard¹,

Froment²,

Ramé³

et al. 2011

Reproduction

View full text Add to dashboard Cite

Resistin, initially identified in adipose tissue and macrophages, was implicated in insulin resistance. Recently, its mRNA was found in hypothalamo-pituitary axis and rat testis, leading us to hypothesize that resistin may be expressed in ovary. In this study, we determined in rats and cows 1) the characterization of resistin in ovary by RT-PCR, immunoblotting, and immunohistochemistry and 2) the effects of recombinant resistin (10, 100, 333, and 667 ng/ml)GIGF1 (76 ng/ml) on steroidogenesis, proliferation, and signaling pathways of granulosa cells (GC) measured by enzyme immunoassay, [3 H]thymidine incorporation, and immunoblotting respectively. We observed that resistin mRNA and protein were present in several bovine and rat ovarian cells. Nevertheless, only bovine GC abundantly expressed resistin mRNA and protein. Resistin treatment decreased basal but not IGF1-induced progesterone (P!0.05; whatever the dose) and estradiol (P!0.005; for 10 and 333 ng/ml) production by bovine GC. In rats, resistin (10 ng/ml) increased basal and IGF1-induced progesterone secretion (P!0.0001), without effect on estradiol release. We found no effect of resistin on rat GC proliferation. Conversely, in cows, resistin increased basal proliferation (P!0.0001; for 100-667 ng/ml) and decreased IGF1-induced proliferation of GC (P!0.0001; for 10-333 ng/ml) associated with a decrease in cyclin D2 protein level (P!0.0001). Finally, resistin stimulated AKT and p38-MAPK phosphorylation in both species, ERK1/2-MAPK phosphorylation in rats and had the opposite effect on the AMPK pathway (P!0.05). In conclusion, our results show that resistin is expressed in rat and bovine ovaries. Furthermore, it can modulate GC functions in basal state or in response to IGF1 in vitro.

show abstract

Comparison of effects of leptin and ghrelin on porcine ovarian granulosa cells

Cited by 35 publications

References 26 publications

Effects of resistin on ovarian folliculogenesis and steroidogenesis in the vespertilionid bat, Scotophilus heathi

Effects of resistin on ovarian folliculogenesis and steroidogenesis in the vespertilionid bat, Scotophilus heathi

Expression and localization of ghrelin and its receptor in ovarian follicles during different stages of development and the modulatory effect of ghrelin on granulosa cells function in buffalo

Expression and effect of resistin on bovine and rat granulosa cell steroidogenesis and proliferation

Contact Info

Product

Resources

About