Comparison of effects of bacterial strains differing in their ability to synthesize cytokinins on growth and cytokinin content in wheat plants

Архипова, Т. Н.; Veselov, S. Yu.; Мелентьев, А. И.; Martynenko, Elena; Kudoyarova, G. R.

doi:10.1134/s1021443706040121

Cited by 17 publications

(7 citation statements)

References 19 publications

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“…The mechanism of this effect is possibly linked to the additional synthesis by Azospirillum of auxin and cytokinin (Arkhipova et al 2006) as growth-promoting hormones that control the functioning of shoot and root apical meristems (Romanov 1992).…”

Section: Discussionmentioning

confidence: 99%

Effect of Azospirillum brasilense Sp245 lipopolysaccharide on the functional activity of wheat root meristematic cells

et al. 2011

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We studied changes in the physiological and biochemical parameters of wheat (Triticum aestivum L. 'Saratovskaya 29') seedlings treated with lipopolysaccharide isolated from the outer membrane of the associative bacterium Azospirillum brasilense Sp245. The obtained data were compared with (i) the results of plant inoculation with whole Sp245 cells and (ii) the effects exerted by the lipopolysaccharide and whole cells of the enterobacterium Escherichia coli K12 and the specific legume symbiont Rhizobium leguminosarum 249. The functional activity of meristematic cells was judged by their mitotic index and by the results of immunochemical determination of the proliferative antigen of initials, a molecular marker for wheat meristem cells. Treatment of the seedling root system with 10 μg mL −1 of Sp245 lipopolysaccharide increased the mitotic index (1.8-fold) and the antigen content (approximately 1.4-fold). These increases were comparable to the effects produced by whole cell inoculation (2-and 1.4-fold, respectively). Our findings give grounds to consider lipopolysaccharide as an active component of the Azospirillum cell surface that not only determines bacterial contact interactions with wheat roots but also participates in the induction of plant responses to these interactions. We finally discuss the linkage between the proliferative antigen of initials and the transduction of a hormonal signal to the cell, as well as the informational value of this antigen as an indicator of effectiveness of plant-bacterial interactions.

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Section: Discussionmentioning

confidence: 99%

Effect of Azospirillum brasilense Sp245 lipopolysaccharide on the functional activity of wheat root meristematic cells

et al. 2011

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“…The ability of rhizobacteria to produce phytohormones has long been believed as a major factor to facilitate plant growth. There are several reports regarding positive effect of bacterial cytokinins and IAA on plant growth (Arkhipova et al 2004;Khalid et al 2004;Arkhipova et al 2005;Arkhipova et al 2006;Teale et al 2006;Arkhipova et al 2007;Idris et al 2007). However, all these reports focus on phytohormone (either IAA or cytokinin) individually.…”

Section: Introductionmentioning

confidence: 92%

Interactions of bacterial cytokinins and IAA in the rhizosphere may alter phytostimulatory efficiency of rhizobacteria

Hussain

Hasnain

2011

World J Microbiol Biotechnol

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Phytohormones from rhizobacterial origin have been linked to their phytostimulation potential. However, while studying the efficacy of plant growth promoting bacteria, focus has always been on a single hormone. The role of plant hormones often overlay and they mutually modulate their effect. In current study focus was on the role of two hormones (cytokinins and indole acetic acid) in phytostimulation by rhizobacteria. Endogenous rhizosphere bacteria were isolated and screened for the presence of phytohormones. Bacterial strains from three different genera (Pseudomonas, Bacillus and Azospirillum) were screened positive for cytokinins and IAA. Phytohormones were simultaneously determined in SPE purified bacterial extract by ultra performance liquid chromatography (UPLC) coupled to a tandem mass spectrometer through electrospray interface. Cytokinins and IAA were determined in positive and negative mode, respectively with MRM scan. Zeatin, zeatin riboside and dihydrozeatin riboside were detected and quantified in the selected strains. Significant positive correlation between cytokinins and IAA in bacterial culture and plant endogenous hormones (r = 0.933 and r = 0.983; P = 0.01, respectively) was observed. However, strains with high IAA to cytokinins ratio could hardly enhance in-planta cytokinins, indicating antagonistic relation between the two hormones. Significant correlation of cytokinin with shoot length (r = 0.797; P = 0.01), fresh weight (r = 0.685; P = 0.01) and dry weight (r = 0.704; P = 0.01) was reported under axenic conditions. Bacterial IAA was correlated negatively to root length (r = 0.853; P = 0.01) and positively correlated to the number of roots (r = 0.964; P = 0.01). In natural conditions maximum increase in spike length (33%), number of tillers (71%) and weight of seeds (39%) was documented at final harvest in bacterially inoculated plants.

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“…Higher chlorophyll content described in wheat plants treated with cytokinin producing bacteria, comparable to chlorophyll levels reached by use of artificial cytokinin BAP (Arkhipova et al, 2006) and by the fact non-producing growth promoting bacteria evaluated at the afore mentioned study were not capable to increase chlorophyll levels nor increase cytokinin level of leaves of bacterizated plants (Arkhipova et al, 2006). In Methylobacterium spp epiphytic bacteria the turnover of isopentenylated tRNA production by bacterium than de novo synthesis of this phytormone (Koenig et al, 2002).…”

Section: Plant Inoculation With Beneficial Microorganisms For Instancmentioning

confidence: 49%

New insights into Plant Growth Promoting Rhizobacterium >i<Bacillus thuringiensis>/i< RZ2MS9 biology: entomopathogenic activity and molecular interaction with >i<Zea mays>/i< L.

Longatto¹

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pela oportunidade de realizar o curso de Doutorado. À minha orientadora profª Drª Maria Carolina Quecine Verdi pela inspiração, acolhimento, compreensão, pragmatismo, bons conselhos e, sobretudo, por acreditar em nosso trabalho, mesmo em meio às mudanças. Ao Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) processo 140590/2017 e ao Programa de Aperfeiçoamento do Ensino (PAE-USP) pelas bolsas concedidas. O presente trabalho foi realizado com apoio da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil (CAPES)-Código de Financiamento 001. Ao professor Dr. João Lucio de Azevedo pela agradável convivência, sabedoria, entusiasmo e bom humor. Ao professor Dr. Roberto Fristche Neto do Laboratório de Plantas Alógamas da ESALQ pela disponibilização das sementes de milho utilizadas nos experimentos de casa de vegetação e da estufa utilizada para secagem de amostras vegetais. À professora Dra. Claudia Barros Monteiro Vitorello pelo apoio e aconselhamento em momentos importantes e por ter disponibilizado equipamento para realização das análises de expressão gênica. Ao professor Dr. José Roberto Postali Parra e a querida técnica Sra Neide Graciano Zério e demais colegas do Laboratório de Biologia de Insetos da ESALQ por terem nos recebido tão bem e fornecido todo suporte para realização dos testes entomológicos. À professora Dra. Maria Lúcia Carneiro Vieira, do Laboratório de Biologia Celular e Molecular de Plantas da ESALQ, pela disponibilização das estufas utilizadas para experimento de casa de vegetação e para preparo de vidraria para extrações de RNA. Aos professores Dr. Fábio Tebaldi Nogueira, Dr. Marcio Castro Silva-Filho e minha orientadora, e colegas Carolina, Filipe, Gleicy, Paula, Renata e Raphael, pelos ensinamentos, conselhos, apoio e atitudes apreendidos durante os dois Estágios Supervisionados em Docência realizados na ESALQ. Ao professor Dr. Antonio Vargas de Oliveira Figueira e Dra. Flavia Bento pelo aprendizado, convivência, respeito e conselhos que levarei comigo da etapa vivida no CENA.

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Comparison of effects of bacterial strains differing in their ability to synthesize cytokinins on growth and cytokinin content in wheat plants

Cited by 17 publications

References 19 publications

Effect of Azospirillum brasilense Sp245 lipopolysaccharide on the functional activity of wheat root meristematic cells

Effect of Azospirillum brasilense Sp245 lipopolysaccharide on the functional activity of wheat root meristematic cells

Interactions of bacterial cytokinins and IAA in the rhizosphere may alter phytostimulatory efficiency of rhizobacteria

New insights into Plant Growth Promoting Rhizobacterium >i<Bacillus thuringiensis>/i< RZ2MS9 biology: entomopathogenic activity and molecular interaction with >i<Zea mays>/i< L.

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