Comparison of Dual Beam Dispersive and FTNIR Spectroscopy for Lactate Detection

Abstract

Near Infrared (800–2500 nm) spectroscopy has been extensively used in biomedical applications, as it offers rapid, in vivo, bed-side monitoring of important haemodynamic parameters, which is especially important in critical care settings. However, the choice of NIR spectrometer needs to be investigated for biomedical applications, as both the dual beam dispersive spectrophotomer and the FTNIR spectrometer have their own advantages and disadvantages. In this study, predictive analysis of lactate concentrations in whole blood were undertaken using multivariate techniques on spectra obtained from the two spectrometer types simultaneously and results were compared. Results showed significant improvement in predicting analyte concentration when analysis was performed on full range spectral data. This is in comparison to analysis of limited spectral regions or lactate signature peaks, which yielded poorer prediction models. Furthermore, for the same region, FTNIR showed 10% better predictive capability than the dual beam dispersive NIR spectrometer.

Capillary electrophoresis with capacitively coupled contactless conductivity detection (C4D) for rapid and simple determination of lactate in sweat

“…Lactate has been determined in biological samples mostly using enzymatic-and nonenzymatic-based sensors [12,[14][15][16][17][18]. However, other methods, such as CE [19][20][21][22], HPLC [23], and spectroscopy [24,25], can also be used. The determination of lactate in sweat has recently been extensively explored using colorimetric [26][27][28][29] and wearable electrochemical sensors [6,8,17,18,30 ].…”

In-Silico Investigation towards the Non-Invasive Optical Detection of Blood Lactate

“…As a step towards the development of such a device, researchers have investigated the feasibility of using short-wave infrared (SWIR) light (typically, between the range of 1300nm -2500nm) to detect the optical signature of lactate in blood and estimated the concentration of lactate from the acquired spectra using various regression models [2][3][4][5][6][7][8] . These preliminary in-vitro investigations have shown great promise and have paved the way for the development of a novel optical sensor for the blood-lactate measurements.…”