Comparison of drug resistance genotypes between Beijing and non-Beijing family strains of Mycobacterium tuberculosis in Korea

Park, Young Kil; Shin, S. J.; Ryu, Sungweon; Cho, Sang Nae; Koh, Won‐Jung; Kwon, O Jung; Shim, Young Soo; Lew, Woo Jin; Bai, Gill Han

doi:10.1016/j.mimet.2005.03.002

Cited by 63 publications

(41 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Excluding the Beijing genotype and three Beijing-like strains, the diversity observed was limited to seven clusters (18.8% of the strains): EAI2-Manilla, Haarlem3, LAM9-var, T1, T1-var, T2, and T3-Osaka. The high percentage of Beijing strains in Okinawa (71.3%) is in agreement with the overall value of 60% reported for East Asian countries (9) but remains one of the highest in this region (23,24,26), with percentages varying from 90% in Beijing and the Hebei province of China (11) to 70% in Korea (24), 68% in Hong Kong (16) and Thailand (25), 54% in Vietnam (1), 44% in Taiwan (14), 33% in peninsular Malaysia (6), and 11% in eastern Malaysia (6). On the other hand, the low proportion of LAM and Haarlem in this study shows the nonexposition of this insular population to any migratory flux from Latin American and European countries.…”

Section: Discussionsupporting

confidence: 89%

Assessment of Mycobacterial Interspersed Repetitive Unit-QUB Markers To Further Discriminate the Beijing Genotype in a Population-Based Study of the Genetic Diversity of Mycobacterium tuberculosis Clinical Isolates from Okinawa, Ryukyu Islands, Japan

et al. 2007

View full text Add to dashboard Cite

The present investigation focused on genetic diversity and drug resistance of 101 Mycobacterium tuberculosis strains isolated between July 2003 and February 2005 in the Okinawa prefecture, Ryukyu Islands, Japan. A high rate of clustering (87%, eight clusters, 2 to 69 strains/cluster) was observed upon spoligotyping; most of it was due to the lower discriminatory power of this method for the Beijing lineage (n ‫؍‬ 72; 71.3% of the isolates). The remaining diversity was limited to seven clusters (two to five isolates/cluster), with the following distribution of major lineages: ill-defined T (n ‫؍‬ 13; 12.8%), ancestral East African-Indian (n ‫؍‬ 6; 5.9%), Haarlem (n ‫؍‬ 4; 4%), Latin American-Mediterranean (n ‫؍‬ 2; 2%), X1 (n ‫؍‬ 1; 1%), and a total absence of the central Asian clade. Three remaining strains could not be classified on the basis of their spoligotype pattern and were labeled "unknown." Subtyping with mycobacterial interspersed repetitive units (MIRUs) in association with additional QUB minisatellites was performed to discriminate among the Beijing strains. Based on an "in-house" spoligotyping/MIRU database (n ‫؍‬ 694 Beijing strains), eight highly discriminative MIRU loci for Beijing strains were selected (loci numbered 10, 16, 23, 26, 27, 31, 39, and 40). The highest discriminatory power (h) observed in our sample (n ‫؍‬ 72; M-26, 0.385; M-10, 0.38; M-31, 0.255; M-16, 0.238) was too low, and 73.6% of the Beijing strains from Okinawa remained clustered. Typing of Beijing strains with additional QUB loci (with the exception of "one-copy" QUB-1451) resulted in higher discriminatory powers: QUB-11b, 0.68; QUB-11a, 0.656; QUB-26, 0.644; QUB-18, 0.553; QUB-4156, 0.5; and QUB-1895, 0.453. A definitive algorithm on the use of QUB markers to subtype Beijing isolates in expanded studies would shed light on their hypervariability, which may sometimes blur recognition between epidemiologically linked Beijing isolates. The total absence of multiple drug resistance among Beijing isolates from Okinawa, as well as the relatively older ages of the patients (majority above 60 years), shows that tuberculosis (TB) is a declining disease in Okinawa, and an adequate TB control program has successfully avoided both the emergence and the spread of multidrug-resistant TB in this insular setting.Understanding the population structure and transmission dynamics of circulating Mycobacterium tuberculosis clades provides a unique insight into crucial public health issues, such as the appearance and persistence of variants escaping human immune response or the emergence of resistance to antibiotics. PCR-based methods, such as spoligotyping (17), may be a good indicator of strain identity by providing information about epidemiologically important clones, particularly when used in a new setting (17,30,40). Although spoligotyping may correctly identify "outbreak" episodes as well as reflect the spread of the disease due to human migratory movements, it is not suitable when used alone for purely epidemiological investigations as it ...

show abstract

Section: Discussionsupporting

confidence: 89%

Assessment of Mycobacterial Interspersed Repetitive Unit-QUB Markers To Further Discriminate the Beijing Genotype in a Population-Based Study of the Genetic Diversity of Mycobacterium tuberculosis Clinical Isolates from Okinawa, Ryukyu Islands, Japan

et al. 2007

View full text Add to dashboard Cite

show abstract

“…However, to apply the reverse hybridization method, it is essential to have the information about the mutation pattern of the genes tested in this method. In addition, re- Although the number of strains analyzed was comparatively small, the findings of this study are similar to those of other studies conducted in Korea [15,16]. For example, the results of a study that used the reverse hybridization method showed that, of the INH-resistant strains, 65.1%…”

Section: Sensitivity and Specificity By Specimen Sourcessupporting

confidence: 87%

“…of the RIF-resistant strains had an rpoB mutation [16]. Molecular diagnostic tests that are designed to target these muta- ined in this study [18].…”

Section: Sensitivity and Specificity By Specimen Sourcesmentioning

confidence: 85%

Detection of Isoniazid and Rifampicin Resistance by Sequencing of katG, inhA, and rpoB Genes in Korea

et al. 2009

View full text Add to dashboard Cite

“…Based on the fingerprinting data, no indication of dual infection was found in these isolates. Dual or multiple mutations in the rpoB have been reported Kazakhstan [37], Turkey [39] Belarus [40] and South Korea [41] to mention a few.…”

Section: Discussionmentioning

confidence: 99%

Extremely high prevalence of multidrug resistant tuberculosis in Murmansk, Russia: a population-based study

Mäkinen¹,

Marjamäki²,

Haanperä-Heikkinen³

et al. 2011

Eur J Clin Microbiol Infect Dis

View full text Add to dashboard Cite

show abstract

Comparison of drug resistance genotypes between Beijing and non-Beijing family strains of Mycobacterium tuberculosis in Korea

Cited by 63 publications

References 26 publications

Assessment of Mycobacterial Interspersed Repetitive Unit-QUB Markers To Further Discriminate the Beijing Genotype in a Population-Based Study of the Genetic Diversity of Mycobacterium tuberculosis Clinical Isolates from Okinawa, Ryukyu Islands, Japan

Assessment of Mycobacterial Interspersed Repetitive Unit-QUB Markers To Further Discriminate the Beijing Genotype in a Population-Based Study of the Genetic Diversity of Mycobacterium tuberculosis Clinical Isolates from Okinawa, Ryukyu Islands, Japan

Detection of Isoniazid and Rifampicin Resistance by Sequencing of katG, inhA, and rpoB Genes in Korea

Extremely high prevalence of multidrug resistant tuberculosis in Murmansk, Russia: a population-based study

Contact Info

Product

Resources

About